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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (2): 279-285.doi: 10.3724/SP.J.1006.2009.00279

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Effect and Action Analysis of qSB-9Tq Conferring Resistance to Rice Sheath Blight

YIN Yue-Jun,ZUO Shi-Min**,WANG Hui,ZHANG Ya-Fang,CHEN Zong-Xiang,MA Yu-Yin,GU Shi-Liang,PAN Xue-Biao*   

  1. Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genetics,Ministry of Education,Yangzhou University, Yangzhou 22509,china
  • Received:2008-07-18 Revised:2008-10-10 Online:2009-02-12 Published:2008-12-11
  • Contact: PAN Xue-Biao

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Abstract:

A quantitative trait locus (QTL) contributing partial resistance to rice sheath blight (SB), named as qSB-9, has been confirmed on chromosome 9 of rice. A rice cultivar, Teqing, possesses the relatively resistant allele, qSB-9Tq, and Lemont has the relatively susceptible allele, qSB-9Le. To evaluate the resistance effect and action mode of qSB-9Tq accurately, a BC6F1 plant, from a backcross of Lemont (recurrent parent)/Teqing, with heterozygous genotype of qSB-9TqLe and similar genetic background to Lemont was screened out by using marker-assisted selection. Artificial inoculation with strong pathogenic Rhizoctonia solani line, RH-9, was conducted on the BC6F2 population and the near-isogenic lines (NILs) selected from BC6F3 and BC6F4 generation in a three-year test. Two experimental designs were adopted. One was completely randomized experiment, in which the plants with three genotypes (qSB-9TqTq, qSB-9LeLe, and qSB-9TqLe) on the locus of qSB-9 were distinguished by detecting marker genotypes flanking the qSB-9 in BC6F2 segregation population. The other was a randomized block design with 3 replicates for three NILs in BC6F3 and BC6F4 generations. Results from the two experiments all suggested that the qSB-9Tq was a dominant resistance allele in the interval between the markers of RM242 and Y92.5, and could reduce disease rating about 1.0 score under ‘0–9’ SB-disease rating system. The result of the research laid a strong foundation for the fine mapping and breeding utilization of the qSB-9Tq.

Key words: Rice(Oryza sativa L.), Sheath blight, QTL, Near-isogenic line, Marker-assisted selection, Resistant effect, Gene action

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