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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (2): 286-294.doi: 10.3724/SP.J.1006.2009.00286

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Mapping of QTL for Embryonic Pigment Components in Brassica napus

QU Cun-Min1,FU Fu-You1,2,LIU Lie-Zhao1,WANG Jia-Feng1,MAO Li-Jia2,YUAN Xiao-Yan1,CHEN Li1,LI Jia-Na1,*   

  1. 1College of Agronomy and Biotechnology, Southwest University, Beibei 400716,China;2Institute of Genetics and Developmental Biology, Chinese Academy of Sciences,Beijing 100101,China
  • Received:2008-06-26 Revised:2008-09-02 Online:2009-02-12 Published:2008-12-11
  • Contact: LI Jia-Na

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Abstract:

The quality of rapeseed oil is greatly affected by embryonic pigments, but it has been little research report on the QTL of embryonic pigment components. The objective of this study was to identify QTL controlling embryonic pigment components in Brassica napus using the composite interval mapping (CIM) method.The recombinant inbred lines (RIL) population selfed for 7 successive generations by single seed propagating was derived from a cross between black-seeded male parent cultivar Zhongyou 821 and yellow-seeded female parent line GH06. The population was grown at Beibei and Wanzhou in Chongqing in 2007. Four kinds of embryonic pigment components were identified by constructed genetic map. A total of 31 QTL were detected on 14 different linkage groups. Five QTL for anthocyanidin content werelocated on linkage groups 1, 5, 10, 16, and 20, respectively, explaining 6.08%–11.67% of phenotypic variation; ten QTL for flavonoid content were located on linkage groups 1, 3, 6, 7, 12, 20, and 25, respectively, explaining 4.48%–11.10% of phenotypic variation; eight QTL for total phenol content were located on linkage groups 1, 2, 12, 16 and 20, respectively, accounting for 5.24%–10.37% of phenotypic variation; and eight QTL for melanin content were located on linkage groups 5, 8, 10, 12, 14, and 22, respectively, explaining 5.44%–11.32% of phenotypic variation. Five major QTL explainingover 10% of phenotypic variation were found, including 2, 1, 1, and 1 for flavonoid, anthocyanidin, total phenol and melanin content (qFCB-3-2, qFCW-1-1, qACW-10-1, qTPCW-12-1, and qMCB-22-5), which accounted for 11.10%, 10.20%, 11.67%, 10.37%, and 11.32% of phenotypic variation respectively. These results suggest that the embryonic pigments are controlled by many minor-effect genes, with a pattern of quantitative trait inheritance, and the expression of the QTL is affected by environmental factors. QTL for seed coat pigments showed little overlapping with those for embryonic pigments, implying that seed coat pigments and embryonic pigments might be controlled by different genetic systems. Molecular markers closely linked with these QTLscould be applied in marker-assisted selection of embryonic pigment components in Brassica napus.

Key words: Brassica napus L, Embryo pigment, Quantitative trait locus(QTL), Recombinant inbred lines(RILs)

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