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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (2): 279-285.doi: 10.3724/SP.J.1006.2009.00279

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Effect and Action Analysis of qSB-9Tq Conferring Resistance to Rice Sheath Blight

YIN Yue-Jun,ZUO Shi-Min**,WANG Hui,ZHANG Ya-Fang,CHEN Zong-Xiang,MA Yu-Yin,GU Shi-Liang,PAN Xue-Biao*   

  1. Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genetics,Ministry of Education,Yangzhou University, Yangzhou 22509,china
  • Received:2008-07-18 Revised:2008-10-10 Online:2009-02-12 Published:2008-12-11
  • Contact: PAN Xue-Biao

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Abstract:

A quantitative trait locus (QTL) contributing partial resistance to rice sheath blight (SB), named as qSB-9, has been confirmed on chromosome 9 of rice. A rice cultivar, Teqing, possesses the relatively resistant allele, qSB-9Tq, and Lemont has the relatively susceptible allele, qSB-9Le. To evaluate the resistance effect and action mode of qSB-9Tq accurately, a BC6F1 plant, from a backcross of Lemont (recurrent parent)/Teqing, with heterozygous genotype of qSB-9TqLe and similar genetic background to Lemont was screened out by using marker-assisted selection. Artificial inoculation with strong pathogenic Rhizoctonia solani line, RH-9, was conducted on the BC6F2 population and the near-isogenic lines (NILs) selected from BC6F3 and BC6F4 generation in a three-year test. Two experimental designs were adopted. One was completely randomized experiment, in which the plants with three genotypes (qSB-9TqTq, qSB-9LeLe, and qSB-9TqLe) on the locus of qSB-9 were distinguished by detecting marker genotypes flanking the qSB-9 in BC6F2 segregation population. The other was a randomized block design with 3 replicates for three NILs in BC6F3 and BC6F4 generations. Results from the two experiments all suggested that the qSB-9Tq was a dominant resistance allele in the interval between the markers of RM242 and Y92.5, and could reduce disease rating about 1.0 score under ‘0–9’ SB-disease rating system. The result of the research laid a strong foundation for the fine mapping and breeding utilization of the qSB-9Tq.

Key words: Rice(Oryza sativa L.), Sheath blight, QTL, Near-isogenic line, Marker-assisted selection, Resistant effect, Gene action

[1]Lee F N, Rush M C. Rice sheath blight: a major rice disease. Plant Dis, 1983, 67: 829-832
[2]Liao H-N(廖皓年), Xiao L-S(肖陵生), Wang H-S(王华生). The occurring and developing history of sheath blight in rice. Guangxi Plant Prot (广西植保), 1997, (3): 35-38 (in Chinese)
[3]Li Z K, Pinson S R M, Marchetti M A, Stansel J W, Park W D. Characterization of quantitative trait loci (QTLs) in cultivated rice contributing to field resistance to sheath blight (Rhizoctonia solani). Theor Appl Genet, 1995, 91: 382-388
[4]Zou J H, Pan X B, Chen Z X, Xu J Y, Lu J F, Zhai W X, Zhu L H. Mapping quantitative trait loci controlling sheath blight resistance in two rice cultivars (Oryza sativa L.). Theor Appl Genet, 2000, 101: 569-575
[5]Kunihiro Y(国广泰史), Qian Q(钱前), Sato H(佐腾宏之), Teng S(滕胜), Zeng D-L(曾大力), Fujimoto K(藤本宽), Zhu L-H(朱立煌). QTL analysis of sheath blight resistance in rice (Oryza sa-tiva L.). Acta Genet Sin (遗传学报), 2002, 29(1): 50-55 (in Chi-nese with English abstract)
[6]Han Y-P(韩月澎), Xing Y-Z(邢永忠), Chen Z-X(陈宗祥), Gu S-L(顾世梁), Pan X-B(潘学彪), Chen X-L(陈秀兰), Zhang Q-F(张启发). Mapping QTLs for horizontal resistance to sheath blight in an elite rice restorer line, Minghui 63. Acta Genet Sin (遗传学报), 2002, 29(7): 565-570 (in Chinese with English ab-stract)
[7]Sato H, Ideta O, Audo I, Kunihiro Y, Hirabayashi H, Iwano M, Miyasaka A, Nemoto H, Imbe T. Mapping QTLs for sheath blight resistance in the rice line WSS2. Breed Sci, 2004, 54: 265-271
[8]Pinson S R M, Capdevielle F M, Oard J H. Confirming QTLs and finding additional loci conditioning sheath blight resistance in rice using recombinant inbred lines. Crop Sci, 2005, 45: 503-510
[9]Tan C-X(谭彩霞), Zhang Y-F(张亚芳), Chen Z-X(陈宗祥), Yin Y-J(殷跃军), Ji X-M(纪雪梅), Yang Y(杨勇), Pan X-B(潘学彪). Identifying of two major quantitative genes controlling sheath blight resistance. China Biotechnol (中国生物工程杂志), 2004, 24(4): 79-80 (in Chinese with English abstract)
[10]Tan C-X(谭彩霞), Ji X-M(纪雪梅), Yang Y(杨勇), Pan X-Y(潘兴元), Zuo S-M(左示敏), Zhang Y-F(张亚芳), Zou J-H(邹军煌), Chen Z-X(陈宗祥), Zhu L-H(朱立煌), Pan X-B(潘学彪). Identi-fication and marker-assisted selection of two major quantitative genes controlling rice sheath blight resistance in backcross gene- rations. Acta Genet Sin (遗传学报), 2005, 32(4): 399-405 (in Chinese with English abstract)
[11]Ji Q, Lu J F, Chao Q, Gu M H, Xu M L. Delimiting a rice wide-compatibility gene S5n to a 50 kb region. Theor Appl Genet, 2005, 111: 1495-1503
[12]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8: 4321-4326
[13]Pan X-B(潘学彪), Chen Z-X(陈宗祥), Xu J-Y(徐敬友), Tong Y-H(童蕴慧), Wang Z-B(王子斌), Pan X-Y(潘兴元). The effects of different methods of inoculation and investigation on genetic research of resistance to rice sheath blight. J Jiangsu Agric Coll (江苏农学院学报), 1997, 18(3): 27-32 (in Chinese with English abstract)
[14]Chen Z-X(陈宗祥), Zou J-H(邹军煌), Han Y-P(韩月澎), Xu J-Y(徐敬友), Tong Y-H(童蕴慧), Yu H-X(于恒秀), Zhang Y-F(张亚芳), Pan X-B(潘学彪). An innovated method for research on inheritance of resistance to rice sheath blight and its verification. Chin J Rice Sci (中国水稻科学), 2002, 16(1): 74-76 (in Chinese with English abstract)
[15]Eizenga G C, Lee F N, Rutger J N. Screening Oryza species plants for rice sheath blight resistance. Plant Dis, 2002, 86: 808-812
[16]Rush M C, Hoff B J, Mcllrath W O. A uniform disease rating system for rice disease in the United States. In: Proc 16th Rice Tech Working Group. Lake Charles, Louisiana, 1976. p 64
[17]Pan X-B(潘学彪), Zhang Y-F(张亚芳), Zuo S-M(左示敏), Chen Z-X(陈宗祥). Discussion on QTLs identification and application for important quantitative traits in crops. J Yangzhou Univ (Agric Life Sci Edn) (扬州大学学报·农业与生命科学版), 2005, 26(2): 50-55 (in Chinese with English abstract)
[18]Yamamoto T, Lin H X, Sasaki T, Yano M. Identification of head-ing date quantitative trait locus Hd6 and characterization of its epistatic interactions with Hd2 in rice using advanced backcross progeny. Genetics, 2000, 154: 885-891
[19]Lin H X, Yamamoto T, Sasaki T, Yano M. Characterization and detection of epistatic interactions of 3 QTLs, Hd1, Hd2, and Hd3, controlling heading date in rice using nearly isogenic lines. Theor Appl Genet, 2000, 101: 1021-1028
[20]Yin Y-J(殷跃军), Zuo S-M(左示敏), Wang H(王辉), Chen Z-X(陈宗祥), Ma Y-Y(马玉银), Zhang Y-F(张亚芳), Gu S-L(顾世梁), Pan X-B(潘学彪). Pyramiding effects of three quantitative trait loci for resistance to sheath blight using near-isogenic lines of rice. Chin J Rice Sci (中国水稻科学), 2008, 22(4): 340-346 (in Chinese with English abstract)
[21]Zuo S M, Zhang L, Wang H, Yin Y J, Zhang Y F, Chen Z X, Ma Y Y, Pan X B. Prospect of the QTL-qSB-9Tq utilized in molecular breeding program of japonica rice against sheath blight. J Genet Genomics, 2008, 35: 499-505
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