Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (2): 279-285.doi: 10.3724/SP.J.1006.2009.00279

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Effect and Action Analysis of qSB-9Tq Conferring Resistance to Rice Sheath Blight

YIN Yue-Jun,ZUO Shi-Min**,WANG Hui,ZHANG Ya-Fang,CHEN Zong-Xiang,MA Yu-Yin,GU Shi-Liang,PAN Xue-Biao*   

  1. Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genetics,Ministry of Education,Yangzhou University, Yangzhou 22509,china
  • Received:2008-07-18 Revised:2008-10-10 Online:2009-02-12 Published:2008-12-11
  • Contact: PAN Xue-Biao

PDF

1616

Cited

2

Abstract:

A quantitative trait locus (QTL) contributing partial resistance to rice sheath blight (SB), named as qSB-9, has been confirmed on chromosome 9 of rice. A rice cultivar, Teqing, possesses the relatively resistant allele, qSB-9Tq, and Lemont has the relatively susceptible allele, qSB-9Le. To evaluate the resistance effect and action mode of qSB-9Tq accurately, a BC6F1 plant, from a backcross of Lemont (recurrent parent)/Teqing, with heterozygous genotype of qSB-9TqLe and similar genetic background to Lemont was screened out by using marker-assisted selection. Artificial inoculation with strong pathogenic Rhizoctonia solani line, RH-9, was conducted on the BC6F2 population and the near-isogenic lines (NILs) selected from BC6F3 and BC6F4 generation in a three-year test. Two experimental designs were adopted. One was completely randomized experiment, in which the plants with three genotypes (qSB-9TqTq, qSB-9LeLe, and qSB-9TqLe) on the locus of qSB-9 were distinguished by detecting marker genotypes flanking the qSB-9 in BC6F2 segregation population. The other was a randomized block design with 3 replicates for three NILs in BC6F3 and BC6F4 generations. Results from the two experiments all suggested that the qSB-9Tq was a dominant resistance allele in the interval between the markers of RM242 and Y92.5, and could reduce disease rating about 1.0 score under ‘0–9’ SB-disease rating system. The result of the research laid a strong foundation for the fine mapping and breeding utilization of the qSB-9Tq.

Key words: Rice(Oryza sativa L.), Sheath blight, QTL, Near-isogenic line, Marker-assisted selection, Resistant effect, Gene action

[1]Lee F N, Rush M C. Rice sheath blight: a major rice disease. Plant Dis, 1983, 67: 829-832
[2]Liao H-N(廖皓年), Xiao L-S(肖陵生), Wang H-S(王华生). The occurring and developing history of sheath blight in rice. Guangxi Plant Prot (广西植保), 1997, (3): 35-38 (in Chinese)
[3]Li Z K, Pinson S R M, Marchetti M A, Stansel J W, Park W D. Characterization of quantitative trait loci (QTLs) in cultivated rice contributing to field resistance to sheath blight (Rhizoctonia solani). Theor Appl Genet, 1995, 91: 382-388
[4]Zou J H, Pan X B, Chen Z X, Xu J Y, Lu J F, Zhai W X, Zhu L H. Mapping quantitative trait loci controlling sheath blight resistance in two rice cultivars (Oryza sativa L.). Theor Appl Genet, 2000, 101: 569-575
[5]Kunihiro Y(国广泰史), Qian Q(钱前), Sato H(佐腾宏之), Teng S(滕胜), Zeng D-L(曾大力), Fujimoto K(藤本宽), Zhu L-H(朱立煌). QTL analysis of sheath blight resistance in rice (Oryza sa-tiva L.). Acta Genet Sin (遗传学报), 2002, 29(1): 50-55 (in Chi-nese with English abstract)
[6]Han Y-P(韩月澎), Xing Y-Z(邢永忠), Chen Z-X(陈宗祥), Gu S-L(顾世梁), Pan X-B(潘学彪), Chen X-L(陈秀兰), Zhang Q-F(张启发). Mapping QTLs for horizontal resistance to sheath blight in an elite rice restorer line, Minghui 63. Acta Genet Sin (遗传学报), 2002, 29(7): 565-570 (in Chinese with English ab-stract)
[7]Sato H, Ideta O, Audo I, Kunihiro Y, Hirabayashi H, Iwano M, Miyasaka A, Nemoto H, Imbe T. Mapping QTLs for sheath blight resistance in the rice line WSS2. Breed Sci, 2004, 54: 265-271
[8]Pinson S R M, Capdevielle F M, Oard J H. Confirming QTLs and finding additional loci conditioning sheath blight resistance in rice using recombinant inbred lines. Crop Sci, 2005, 45: 503-510
[9]Tan C-X(谭彩霞), Zhang Y-F(张亚芳), Chen Z-X(陈宗祥), Yin Y-J(殷跃军), Ji X-M(纪雪梅), Yang Y(杨勇), Pan X-B(潘学彪). Identifying of two major quantitative genes controlling sheath blight resistance. China Biotechnol (中国生物工程杂志), 2004, 24(4): 79-80 (in Chinese with English abstract)
[10]Tan C-X(谭彩霞), Ji X-M(纪雪梅), Yang Y(杨勇), Pan X-Y(潘兴元), Zuo S-M(左示敏), Zhang Y-F(张亚芳), Zou J-H(邹军煌), Chen Z-X(陈宗祥), Zhu L-H(朱立煌), Pan X-B(潘学彪). Identi-fication and marker-assisted selection of two major quantitative genes controlling rice sheath blight resistance in backcross gene- rations. Acta Genet Sin (遗传学报), 2005, 32(4): 399-405 (in Chinese with English abstract)
[11]Ji Q, Lu J F, Chao Q, Gu M H, Xu M L. Delimiting a rice wide-compatibility gene S5n to a 50 kb region. Theor Appl Genet, 2005, 111: 1495-1503
[12]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8: 4321-4326
[13]Pan X-B(潘学彪), Chen Z-X(陈宗祥), Xu J-Y(徐敬友), Tong Y-H(童蕴慧), Wang Z-B(王子斌), Pan X-Y(潘兴元). The effects of different methods of inoculation and investigation on genetic research of resistance to rice sheath blight. J Jiangsu Agric Coll (江苏农学院学报), 1997, 18(3): 27-32 (in Chinese with English abstract)
[14]Chen Z-X(陈宗祥), Zou J-H(邹军煌), Han Y-P(韩月澎), Xu J-Y(徐敬友), Tong Y-H(童蕴慧), Yu H-X(于恒秀), Zhang Y-F(张亚芳), Pan X-B(潘学彪). An innovated method for research on inheritance of resistance to rice sheath blight and its verification. Chin J Rice Sci (中国水稻科学), 2002, 16(1): 74-76 (in Chinese with English abstract)
[15]Eizenga G C, Lee F N, Rutger J N. Screening Oryza species plants for rice sheath blight resistance. Plant Dis, 2002, 86: 808-812
[16]Rush M C, Hoff B J, Mcllrath W O. A uniform disease rating system for rice disease in the United States. In: Proc 16th Rice Tech Working Group. Lake Charles, Louisiana, 1976. p 64
[17]Pan X-B(潘学彪), Zhang Y-F(张亚芳), Zuo S-M(左示敏), Chen Z-X(陈宗祥). Discussion on QTLs identification and application for important quantitative traits in crops. J Yangzhou Univ (Agric Life Sci Edn) (扬州大学学报·农业与生命科学版), 2005, 26(2): 50-55 (in Chinese with English abstract)
[18]Yamamoto T, Lin H X, Sasaki T, Yano M. Identification of head-ing date quantitative trait locus Hd6 and characterization of its epistatic interactions with Hd2 in rice using advanced backcross progeny. Genetics, 2000, 154: 885-891
[19]Lin H X, Yamamoto T, Sasaki T, Yano M. Characterization and detection of epistatic interactions of 3 QTLs, Hd1, Hd2, and Hd3, controlling heading date in rice using nearly isogenic lines. Theor Appl Genet, 2000, 101: 1021-1028
[20]Yin Y-J(殷跃军), Zuo S-M(左示敏), Wang H(王辉), Chen Z-X(陈宗祥), Ma Y-Y(马玉银), Zhang Y-F(张亚芳), Gu S-L(顾世梁), Pan X-B(潘学彪). Pyramiding effects of three quantitative trait loci for resistance to sheath blight using near-isogenic lines of rice. Chin J Rice Sci (中国水稻科学), 2008, 22(4): 340-346 (in Chinese with English abstract)
[21]Zuo S M, Zhang L, Wang H, Yin Y J, Zhang Y F, Chen Z X, Ma Y Y, Pan X B. Prospect of the QTL-qSB-9Tq utilized in molecular breeding program of japonica rice against sheath blight. J Genet Genomics, 2008, 35: 499-505
[1] Zheng Yu-Zhen, Qi Fei-Yan, Sun Zi-Qi, Liu Hua, Qin Li, Shi Lei, Wang Juan, Wang Meng-Meng, Han Suo-Yi, Xu Jing, Miao Li-Juan, Huang Bing-Yan, Dong Wen-Zhao, Zheng Zheng, Zhang Xin-You. QTL mapping of total very long-chain fatty acids and seven fatty acid components in peanut seeds [J]. Acta Agronomica Sinica, 2026, 52(6): 1646-1657.
[2] Liu Chang-You, Wang Shen, Shi Hui-Ying, Shen Ying-Chao, Sun Lei, Wang Yan, Zhang Zhi-Xiao, Su Qiu-Zhu, Tian Jing, Fan Bao-Jie. QTL mapping for bruchid resistance in an adzuki bean distant hybridization population using rice bean genetic resources [J]. Acta Agronomica Sinica, 2026, 52(3): 936-944.
[3] ZHANG Fei-Fei, HE Wan-Long, JIAO Wen-Juan, BAI Bin, GENG Hong-Wei, CHENG Yu-Kun. Meta-analysis of stripe rust resistance-associated traits and candidate gene identification in wheat [J]. Acta Agronomica Sinica, 2025, 51(8): 2111-2127.
[4] HU Meng, SHA Dan, ZHANG Sheng-Rui, GU Yong-Zhe, ZHANG Shi-Bi, LI Jing, SUN Jun-Ming, QIU Li-Juan, LI Bin. QTL mapping and candidate gene screening for branch number in soybean [J]. Acta Agronomica Sinica, 2025, 51(7): 1747-1756.
[5] SHAO Shun-Wei, CHEN Zhuo, LAN Zhen-Dong, CAI Xing-Kui, ZOU Hua-Fen, LI Chen-Xi, TANG Jing-Hua, ZHU Xi, ZHANG Yu, DONG Jian-Ke, JIN Hui, SONG Bo-Tao. QTL mapping of tuber eye depth based on BSA-seq technique [J]. Acta Agronomica Sinica, 2025, 51(7): 1725-1735.
[6] YANG Hai-Yang, WU Lin-Xuan, LI Bo-Wen, SHI Han-Feng, YUAN Xi-Long, LIU Jin-Zhao, CAI Hai-Rong, CHEN Shi-Yi, GUO Tao, WANG Hui. OsWRI3, identified based on QTL mapping, regulates seed shattering in rice [J]. Acta Agronomica Sinica, 2025, 51(7): 1712-1724.
[7] YANG Xiao-Hui, YAN Xuan-Jun, YANG Wen-Yan, FU Jun-Jie, YANG Qin, XIE Yu-Xin. Effect evaluation and investigation on molecular mechanism of the ZmKL1 favorable allele in regulating maize kernel size [J]. Acta Agronomica Sinica, 2025, 51(6): 1501-1513.
[8] GUO Dong-Cai, LYU Tao, CAI Yong-Sheng, MAI WU-LU-DA·AI He-Mai-Ti, CHEN Quan-Jia, QU Yan-Ying, ZHENG Kai. Meta-analysis of QTL and identification of candidate genes for fiber quality in cotton [J]. Acta Agronomica Sinica, 2025, 51(6): 1445-1466.
[9] ZHANG Jin-Ze, ZHOU Qing-Guo, XIAO Li-Jing, JIN Hai-Run, OU-YANG Qing-Jing, LONG Xu, YAN Zhong-Bin, TIAN En-Tang. QTL mapping and candidate gene analysis of glucosinolate content in various tissues of Brassica juncea [J]. Acta Agronomica Sinica, 2025, 51(5): 1166-1177.
[10] LIN Wei-Jin, GUO Ze-Jia, LIU Hao, LI Hai-Fen, WANG Run-Feng, HUANG Lu, YU Qian-Xia, CHEN Xiao-Ping, HONG Yan-Bin, LI Shao-Xiong, LU Qing. QTL mapping and candidate gene analysis of peanut pod yield-related traits [J]. Acta Agronomica Sinica, 2025, 51(4): 969-981.
[11] WANG Xiao-Lin, LIU Zhong-Song, KANG Lei, YANG Liu. Mapping of silique length and seeds per silique and transcriptome profiling of pod walls in Brassica napus L. [J]. Acta Agronomica Sinica, 2025, 51(4): 888-899.
[12] YONG Rui, HU Wen-Jing, WU Di, WANG Zun-Jie, LI Dong-Sheng, ZHAO Die, YOU Jun-Chao, XIAO Yong-Gui, WANG Chun-Ping. Identification and validation of quantitative trait loci for grain number per spike showing pleiotropic effect on thousand grain weight in bread wheat (Triticum aestivum L.) [J]. Acta Agronomica Sinica, 2025, 51(2): 312-323.
[13] HU Peng-Ju, GUO Song, SONG Ya-Hui, JIN Xin-Xin, SU Qiao, YANG Yong-Qing, WANG Jin. Genetic and QTL mapping analysis of oil content in peanut across multiple environments [J]. Acta Agronomica Sinica, 2025, 51(2): 324-333.
[14] GUO Shu-Hui, PAN Zhuan-Xia, ZHAO Zhan-Sheng, YANG Liu-Liu, HUANG-FU Zhang-Long, GUO Bao-Sheng, HU Xiao-Li, LU Ya-Dan, DING Xiao, WU Cui-Cui, LAN Gang, LYU Bei-Bei, TAN Feng-Ping, LI Peng-Bo. Genetic analysis of a major fiber length locus on chromosome D11 of upland cotton [J]. Acta Agronomica Sinica, 2025, 51(2): 383-394.
[15] WANG Zhe, HU Yan-Ling, GONG Fang-Yi, YI Rui, ZHAO Shu-Hong, LIU Rui-Qin, LIU Yu-Hang, ZHANG Tian, ZHANG Ya-Zhou, ZHENG You-Liang, LIU Deng-Cai, HUANG Lin, WU Bi-Hua. QTL mapping of grain protein content in the introgression line BAd7-209 derived from wild emmer [J]. Acta Agronomica Sinica, 2025, 51(12): 3238-3250.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd