Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (6): 1139-1145.doi: 10.3724/SP.J.1006.2009.01139

• RESEARCH NOTES • Previous Articles     Next Articles

Comparison of Seed Structure of Progenies from Intergeneric Hybrids between Sinapis alba and Brassica napus

WEI Cun-Xu1,LI Ai-Min12,ZHANG Yong-Tai2,ZHOU Wei-Dong3,WANG You-Ping1*   

  1. 1College of Bioscience and Biotechnology,Yangzhou University,Yangzhou 225009,China;2Jiangsu Institute of Agricultural Science in the Lixiahe district,Yangzhou 225007,China;3Analytical Centre,Yangzhou University,Yangzhou 225009,China
  • Received:2008-11-27 Revised:2009-02-17 Online:2009-06-12 Published:2009-04-16
  • Contact: WANG You-Ping，E-mail:wangyp@yzu.edu.cn

PDF

1670

Cited

2

Abstract:

Sinapis alba L. possesses desirable agronomic characters. The backcross progenies of intergeneric hybrids between S. alba and Brassica napus L. obtained the valuable agronomic traits from S. alba. Seed structure of some yellow and yellow-like seed lines, which were selected from backcross progenies, was observed under light microscopy and electronic microscopy. The morphologically anatomical structure of seed coat of backcross progenies resembled that of B. napus, but was different from that of S. alba. Seed coat of B. napus was composed of flattened epidermis, crushed subepidermis, U-shaped-a palisade layer and aleurone layer, while seed coat of S. alba was composed of intestine-shaped epidermis, ellipse subepidermis, U-shaped-b palisade layer and aleurone layer. The pigments of seed coat were mainly distributed in the palisade layer. There were pigments in seed coats of B. napus and some backcross progenies, but no pigments in those of S. alba and other backcross progenies. The palisade layer was thicker in B. napus, thinner in S. alba, and intermediate in hybrid progenies. Seed coat microsculpturing was observed with scanning electronic microscope. The reticulate-foveate pattern was the characteristics of B. napus and hybrid progenies, while the blister pattern of H2O-immersed seed and the sulcate pattern of dry seed were the characteristics of S. alba. The area of cotyledon cell was larger in B. napus, smaller in S. alba, and intermediate in hybrid progenies. The area index of protein body in cotyledon cell was higher in S. alba, lower in B. napus, and intermediate in progenies. The observations of ultrastructure showed that protein bodies were heterogeneous with crystal globoids in parents and progenies. Oilbodies included large and small oilbodies, and their sizes were different from each other between parents and progenies. The above results showed that the anatomy of the progeny seeds was similar to that of B. napus, but the seed color, the thickness of palisade layer, cotyledon cell size, protein body and oilbody were affected by S. alba.

Key words: Sinapis alba L., Brassica napus L., Progeny of somatic hybrids, Seed structure, Yellow seed

[1] Liu H-L(刘后利). Origin and evolution of some Brassicas. Acta Agron Sin(作物学报), 1984, 10(1): 9-18(in Chinese with English abstract)

[2] Yan M-L(严明理). Studies on the molecular mechanism of formation of yellow seeds in Brassica juncea. PhD Dissertation of Hunan University (湖南农业大学), 2007. p 1 (in Chinese with English abstract)

[3] Downey R K. Rapeseed and Mustard. In: Fehr W R ed. Principles of Cultivar Development. New York: Macmillan Publishing Company, 1987. pp 437-486

[4] Wang Y P, Sonntag K, Rudloff E, Chen J M. Intergeneric somatic hybridization between Brassica napus and Sinapis alba. Integr J Plant Biol, 2005, 47: 84-91

[5] Bodnaryk R P, Lamb R J. Mechanisms of resistance to the flea beetle, Phyllotreta cruciferae (Goeze), in yellow mustard seedlings, Sinapis alba L. Can J Plant Sci, 1991, 71: 13-20

[6] Hansen L N, Earle E D. Somatic hybrids between Brassica oleracea L. and Sinapis alba L. with resistance to Alternaria brassicae (Berk.) Sacc. Theor Appl Genet, 1997, 94: 1078-1085

[7] Lelivelt C L C, Leunissen E H M, Frederiks H J, Helsper J P F C, Krens F A. Transfer of resistance to the beet cyst nematode (Heterodera schachtii Schm.) from Sinapis alba L. (white mustard) to the Brassica napus L. gene pool by sexual and somatic hybridization. Theor Appl Genet, 1993, 85: 688-696

[8] Brown J, Brown A P, Davis J B, Erickson D. Intergeneric hybridization between Sinapis alba and Brassica napus. Euphytica, 1997, 93: 163-168

[9] Chevre A M, Eber F, Margale E, Kerlan M C, Primard C, Vedel F, Delseny M, Pelletier G. Comparison of somatic and sexual Brassica napus-Sinapis alba hybrids and their progeny by cytogenetic studies and molecular characterization. Genome, 1994, 37: 367-374

[10] Wang Y P, Zhao X X,Sonntag K,Wehling P, Snowdon R J. Behaviour of Sinapis alba addition chromosomes in a Brassica napus background revealed by genomic in situ hybridisation. Chromosome Res, 2005, 13: 819-826

[11] Hu S-Y(胡适宜), Xu L-Y(徐丽云). A cytochemical technique for demonstration of lipids, polysaccharides and protein bodies in thick resin sections. Acta Bot Sin (植物学报), 1990, 32(11): 841-846 (in Chinese with English abstract)

[12] Buth G M, Ara R. Seed coat anatomy of some cultivated Brassica. Phytomorphology, 1981, 31: 69-78

[13] Zeng C L, Wang J B, Liu A H, Wu X M. Seed coat microsculpturing changes during seed development in diploid and amphidiploid Brassica species. Ann Bot, 2004, 93: 555-566

[14] Koul K K, Nagpal R, Raina S N. Seed coat microsculpturing in Brassica and allied genera (Subtribes Brassicinae Raphaninae, Moricandiinae). Ann Bot, 2000, 86: 385-397

[15] Harris W M. Seed coat development in radish (Raphanus sativus L.). Phytomorphology, 1991, 41: 341-349

[16] Windsor J B, Symonds V V, Mendenhall J, Lloyd A M. Arabidopsis seed coat development: morphological differentiation of the outer integument. Plant J, 2000, 22: 483-493

[17] Bouman F. Integument initiation and testa development in some Cruciferae. Bot J Linn Soc, 1975, 70: 213-299

[18] Vaughan J G, Whitehouse J M. Seed structure and the taxonomy of the Cruciferae. Bot J Linn Sci, 1971, 64: 383-409

[19] Wan L L, Xia Q, Qiu X, Selvaraj G. Early stages of seed development in Brassica napus: A seed coat-specific cysteine proteinase associated with programmed cell death of the inner integument. Plant J, 2002, 30: 1-10

[20] Marles M A S, Gruber M Y. Histochemical characterization of unextractable seed coat pigments and quantification of extractable lignin in the Brassicaceae. J Sci Food Agric, 2004, 84: 251-262

[21] Li C-H(李崇辉), Chen W-Y(陈文艺). An investigation on the distribution of rape yellow seeds and its variation in seed coat colour. J Southwest Agric Uni(西南农业大学学报), 1998, 20(3): 256-259 (in Chinese with English abstract)

[22] Lott J N A. Protein bodies in seeds. Nord J Bot, 1981, 1: 421-423

[23] Chen P, Lott J N A. Studies of Capsicum annuum L. seeds: structure, storage reserves and mineral nutrients. Can J Bot, 1992, 70: 518-529

[24] Tzen J T C, Cao Y Z, Laurent P, Ratnayake C, Huang A H C. Lipids, proteins and structure of seed oilbodies from diverse species. Plant Physiol, 1993, 101: 267-276

[25] Rodrigo M P S, Findlay K, Lopez-Villalobos A, Yeung E C, Nykiforuk C L, Moloney M M. The accumulation of oleosins determines the size of seed oilbodies in Arabidopsis. Plant Cell, 2006, 18: 1961-1974

[26] Ting J T L, Lee K Y, Ratnayake C, Platt K A, Balsamo R A, Huang A H C. Oleosin genes in maize kernels having diverse oil contents are constitutively expressed independent of oil contents: size and shape of intracellular oilbodies are determined by the oleosins oils ratio. Planta, 1996, 199: 158-165
[1] ZHANG Yu-Kun, LU Ying, CUI Kan, XIA Shi-Tou, LIU Zhong-Song. Allelic variation and geographical distribution of TT8 for seed color in Brassica juncea Czern. et Coss. [J]. Acta Agronomica Sinica, 2022, 48(6): 1325-1332.
[2] WANG Wen-Xiang,HU Qiong,MEI De-Sheng,LI Yun-Chang,ZHOU Ri-Jin,WANG Hui,CHENG Hong-Tao,FU Li,LIU Jia*. Genetic Effects of Branch Angle Using Mixture Model of Major Gene Plus Polygene in Brassica napus L. [J]. Acta Agron Sin, 2016, 42(08): 1103-1111.
[3] LU Kun,QU Cun-Min,LI Sha,ZHAO Hui-Yan,WANG Rui,XU Xin-Fu,LIANG Ying,LI Jia-Na. Expression Analysis and eQTL Mapping of BnTT3 Gene in Brassica napus L. [J]. Acta Agron Sin, 2015, 41(11): 1758-1766.
[4] JIAO Cong-Cong,HUANG Ji-Xiang,WANG Yi-Long,ZHANG Xiao-Yu,XIONG Hua-Xin,NI Xi-Yuan,ZHAO Jian-Yi. Genetic Analysis of Yield-Associated Traits by Unconditional and Conditional QTL in Brassica napus [J]. Acta Agron Sin, 2015, 41(10): 1481-1489.
[5] TANG Min-Qiang,CHENG Xiao-Hui,TONG Chao-Bo,LIU Yue-Ying,ZHAO Chuan-Ji,DONG Cai-Hua,YU Jing-Yin,MA Xiao-Gen,HUANG Jun-Yan,LIU Sheng-Yi. Genome-wide Association Analysis of Plant Height in Rapeseed (Brassica napus) [J]. Acta Agron Sin, 2015, 41(07): 1121-1126.
[6] ZHANG Ya-Jie,LI Jing,PENG Hong-Kun,CHEN Xiu-Bin,ZHENG Hong-Yu,CHEN Sheng-Bei,LIU An-Guo,HU Li-Yong. Dynamic Simulation Model for Growth Duration of Rapeseed (Brassica napus) [J]. Acta Agron Sin, 2015, 41(05): 766-777.
[7] Lü Yan-Yan,FU San-Xiong,CHEN Song,ZHANG Wei,QI Cun-Kou*. Cloning of BnADH3 Gene from Brassica napus L. and Submergence Tolerance of BnADH3 Transgenic Arabidopsis [J]. Acta Agron Sin, 2015, 41(04): 565-573.
[8] ZHANG Wei-Xin,CAO Hong-Xin,ZHU Yan,LIU Yan,ZHANG Wen-Yu,CHEN Yu-Li,FU Kun-Ya. Morphological Structure Model of Leaf Space Based on Biomass at Pre-Overwintering Stage in Rapeseed (Brassica napus L.) Plant [J]. Acta Agron Sin, 2015, 41(02): 318-328.
[9] WEN Juan,XU Jian-Feng,LONG Yan,XU Hai-Ming,MENG Jin-Ling,WU Jian-Guo,SHI Chun-Hai. QTL Mapping and Analysis Based on Embryo and Maternal Genetic Systems for Semi-Essential Amino Acid Contents in Rapeseed (Brassica napus L.) [J]. Acta Agron Sin, 2015, 41(01): 57-65.
[10] JIN Yan,Lü Yan-Yan,FU San-Xiong,QI Cun-Kou. Inheritance of Major Gene Plus Polygene of Water-logging Tolerance in Brassica napus L. [J]. Acta Agron Sin, 2014, 40(11): 1964-1972.
[11] QU Cun-Min,LU Kun,LIU Shui-Yan,BU Hai-Dong,FU Fu-You,WANG Rui,XU Xin-Fu,LI Jia-Na. SNP Detection and Analysis of Genes for Flavonoid Pathway in Yellow- and Black-Seeded Brassica napus L. [J]. Acta Agron Sin, 2014, 40(11): 1914-1924.
[12] ZHOU Qing-Yuan,CUI Cui,YIN Tao,CHEN Dong-Liang,ZHANG Zheng-Sheng,LI Jia-Na. Genetic Analysis of Silique Length Using Mixture Model of Major Gene Plus Polygene in Brassica napus L. [J]. Acta Agron Sin, 2014, 40(08): 1493-1500.
[13] ZHAO Hui-Yan,XIAO Lu,ZHAO Zhi,DU De-Zhi*. Development of Molecular Markers and Map Integration for Seed Color Traits in Dahuang Rape (Brassica rapa L.) [J]. Acta Agron Sin, 2014, 40(06): 965-972.
[14] ZUO Qing-Song,YANG Hai-Yan,LENG Suo-Hu,CAO Shi,ZENG Jiang-Xue,WU Jiang-Sheng,ZHOU Guang-Sheng. Effects of Nitrogen Fertilizer on Nitrogen Accumulation, Translocation and Nitrogen Use Efficiency in Rapeseed (Brassica napus L.) [J]. Acta Agron Sin, 2014, 40(03): 511-518.
[15] HU Mao-Long,LONG Wei-Hua,GAO Jian-Qin,FU San-Xiong,CHEN Feng,ZHOU Xiao-Yin,PENG Qi,ZHANG Wei,PU Hui-Ming*,QI Cun-Kou,ZHANG Jie-Fu,CHEN Song. Development and Application of Allele-Specific PCR Markers for Imidazolinone-Resistant Gene BnALS1R in Brassica napus [J]. Acta Agron Sin, 2013, 39(10): 1711-1719.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd