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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (6): 1139-1145.doi: 10.3724/SP.J.1006.2009.01139

• RESEARCH NOTES • Previous Articles     Next Articles

Comparison of Seed Structure of Progenies from Intergeneric Hybrids between Sinapis alba and Brassica napus

WEI Cun-Xu1,LI Ai-Min12,ZHANG Yong-Tai2,ZHOU Wei-Dong3,WANG You-Ping1*   

  1. 1College of Bioscience and Biotechnology,Yangzhou University,Yangzhou 225009,China;2Jiangsu Institute of Agricultural Science in the Lixiahe district,Yangzhou 225007,China;3Analytical Centre,Yangzhou University,Yangzhou 225009,China
  • Received:2008-11-27 Revised:2009-02-17 Online:2009-06-12 Published:2009-04-16
  • Contact: WANG You-Ping，E-mail:wangyp@yzu.edu.cn

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Abstract:

Sinapis alba L. possesses desirable agronomic characters. The backcross progenies of intergeneric hybrids between S. alba and Brassica napus L. obtained the valuable agronomic traits from S. alba. Seed structure of some yellow and yellow-like seed lines, which were selected from backcross progenies, was observed under light microscopy and electronic microscopy. The morphologically anatomical structure of seed coat of backcross progenies resembled that of B. napus, but was different from that of S. alba. Seed coat of B. napus was composed of flattened epidermis, crushed subepidermis, U-shaped-a palisade layer and aleurone layer, while seed coat of S. alba was composed of intestine-shaped epidermis, ellipse subepidermis, U-shaped-b palisade layer and aleurone layer. The pigments of seed coat were mainly distributed in the palisade layer. There were pigments in seed coats of B. napus and some backcross progenies, but no pigments in those of S. alba and other backcross progenies. The palisade layer was thicker in B. napus, thinner in S. alba, and intermediate in hybrid progenies. Seed coat microsculpturing was observed with scanning electronic microscope. The reticulate-foveate pattern was the characteristics of B. napus and hybrid progenies, while the blister pattern of H2O-immersed seed and the sulcate pattern of dry seed were the characteristics of S. alba. The area of cotyledon cell was larger in B. napus, smaller in S. alba, and intermediate in hybrid progenies. The area index of protein body in cotyledon cell was higher in S. alba, lower in B. napus, and intermediate in progenies. The observations of ultrastructure showed that protein bodies were heterogeneous with crystal globoids in parents and progenies. Oilbodies included large and small oilbodies, and their sizes were different from each other between parents and progenies. The above results showed that the anatomy of the progeny seeds was similar to that of B. napus, but the seed color, the thickness of palisade layer, cotyledon cell size, protein body and oilbody were affected by S. alba.

Key words: Sinapis alba L., Brassica napus L., Progeny of somatic hybrids, Seed structure, Yellow seed

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