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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (05): 779-787.doi: 10.3724/SP.J.1006.2010.00779

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Expression Analysis of TaPhyB3 in Triticum aestivum

LI Zhuang1,2, MA Yan-Bin1,2,**,CAI Ying-Fan3,WU Suo-Wei2,XIAO Yang4,MENG Fan-Hua2,FU Feng-Ling1,HUANG Yu-Bi1,*,YANGJian-Ping2,3,*   

  1. 1 Maize Research Institute, Sichuan Agricultural University, Ya’an 450002, China; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 College of Bio-information, Chongqing University of Posts and Tele-communication, Chongqing 400065, China; 4 Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2009-11-26 Revised:2010-02-08 Online:2010-05-12 Published:2010-03-15
  • Contact: YANG Jian-Ping,E-mail:yangjianping@caas.net.cn,Tel:010-82105859;HUANG Yu-Bi,E-mail:yubihuang@sohu.com,Tel: 0835-2882331 E-mail:李壮, lizhuang2006@sina.com, Tel: 010-82105851

Abstract:

As an important regulator in growth, development, and metabolic activities of plant, light is perceived by a variety of photoreceptors that control developmental processes, such as germination, photomorphogenesis, flowering, and senescence. Phytochromes play a pivotal role in plant adaptability to ambient environment. AtPhyB has been found to be involved in the response to red light. At present, PhyB genes have been cloned in various plants, and the expression patterns and functions of the gene family have been studied in Arabidopsis thaliana, rice (Oryza sativa L.), and maize (Zea mays L.), but not in wheat (Triticum aestivum L.). The objectives of this study were to clone the full length of PhyB and study its structure and expression under different lights. The full-length cDNA sequence of PhyB, encoding the apoproteinof phytochrome B, was cloned from wheat cultivar Chinese Spring. This gene is located on chromosome 4D and designated TaPhyB3. This gene possesses four extrons and three introns and the open reading frame TaPhyB3 is 3 501 bases in length, which encodes predicted protein of 1 166 amino acids. The conserved domains of PhyB gene family, i.e., DAF-DOMAIN, PHYTOCHROME REGION, PAS-A DOMAIN, PAS-B DOMAIN, HISTIDINE RELATED DOMAIN 1, and HISTIDINE RELATED DOMAIN 2, were also observed in the predicted protein sequence. The alignment analysis of amino acid sequence showed that TaPhyB3 shared 93% or 90% identity with the PHYBs of rice or maize, respectively, but only 73% with that of Arabidopsis. After treated with continuous darkness, far-red, red, blue, and white lights for 7 d, young seedlings of Chinese Spring were sampled for TaPhyB3 expression analysis using real-time reverse transcription polymerase chain reaction (RT-PCR). TaPhyB3 expression levels in the wheat seedlings under far-red, red, blue, and white lights were 2.2, 7.7, 7.4, and 37.3 times as high as that in seedlings under darkness. When exposed to white light for 90 d, the TaPhyB3 expression was detected in root, stem, leaf, and spike. However, the gene was mainly expressed in above-ground organs of wheat seedling, and TaPhyB3 expression level in leaf was 11.4 times as high as that in root. The expression level of TaPhyB3 is speculated to positively correlate with the degree of the seedling photomorphogenesis.

Key words: Triticum aestivum, Phytochrome B, Gene cloning, Gene expression

[1] Kendrick R E, Kronenberg G H M. Photomorphogenesis in plants. Dordrecht, the Netherlands: Kluwer Academic Publishers, 1994

[2] Briggs W R, Olney M A. Photoreceptors in plant photomorphogenesis to date: Five phytochromes, two cryptochromes, one phototropin, and one superchrome. Plant Physiol, 2001, 125: 85–88

[3] Rockwell N C, Su Y S, Lagarias J C. Phytochrome structure and signaling mechanisms. Annu Rev Plant Biol, 2006, 57: 837–858

[4] Briggs W R, Christie J M. Phototropins 1 and 2: Versatile plant blue-light receptors. Trends Plant Sci, 2002, 7: 204–210

[5] Lin C, Todo T. The cryptochromes. Genome Biol, 2005, 6: 220

[6] Ballaré C L. Stress under the sun: Spotlight on ultraviolet-B response. Plant Physiol, 2003, 132: 1725–1727

[7] Sage LC. Pigment of the Imagination: A history of Phytochrome Research. San Diego, CA, USA: Academic Press, 1992. p 562

[8] Sharrock R A, Quail P H. Novel phytochrome sequences in Arabidopsis thaliana: Structure, evolution, and differential expression of a plant regulatory photoreceptor family. Genes Dev, 1989, 3: 1745–1757

[9] Somers D E, Quail P H. Temporal and spatial expression patterns of PHYA and PHYB genes in Arabidopsis. Plant J, 1995, 7: 413–427

[10] Canton F R, Quail P H. Both phyA and phyB mediate light-imposed repression of PHYA gene expression in Arabidopsis. Plant Physiol, 1999, 121: 1207–1215

[11] Clough R C, Jordan-Beebe E T, Lohman K N, Marita J M, Walker J M, Gatz C, Vierstra R D. Sequences within the N- and C-terminal domains of phytochrome A are required for PFR ubiquitination and degradation. Plant J, 1999 17: 155–167

[12] Hennig L, Büche C, Eichenberg K, Schäfer E. Dynamic properties of endogenous phytochrome A in Arabidopsis seedlings. Plant Physiol, 1999, 121: 571–577

[13] Clack T, Mathews S, Sharrock R A. The phytochrome apoprotein family in Arabidopsis is encoded by five genes-the sequences and expression of PHYD and PHYE. Plant Mol Biol, 1994, 25: 413–427

[14] Hirschfeld M, Tepperman J M, Clack T, Quail P H, Sharrock R A. Coordination of phytochrome levels in PhyB mutants of Arabidopsis as revealed by apoproteinspecific monoclonal antibodies. Genetics, 1998, 149: 523–535

[15] Abdul-Kader J, Konjevic R, Whitelam G, Gordon W, Poff L K. Both phytochrome A and phytochrome B are required for the normal expression of phototropism in Arabidopsis thaliana seedlings. Physiol Plant, 1997, 101: 278–282

[16] Whitelam G C, Devlin P F. Roles of different phytochromes in Arabidopsis photomorphogenesis. Plant Cell Environ, 1997, 20: 752–758

[17] Folta K M, Spalding E P. Opposing roles of phytochrome A and phytochrome B in early cryptochrome-mediated growth inhibition. Plant J, 2001, 28: 333–340

[18] Tepperman J M, Hudson M E, Khanna R, Zhu T, Chang S H, Wang X, Quail P H. Expression profiling of phyB mutant demonstrates substantial contribution of other phytochromes to red-light-regulated gene expression during seedling deetiolation. Plant J, 2004, 38: 725–739

[19] Takano M, Inagaki N, Xie X, Yuzurihara N, Hihara F, Ishizuka T, Yano M, Nishimura M, Miyao A, Hirochika H, Shinomura T. Distinct and cooperative functions of phytochromes A, B, and C in the control of deetiolation and flowering in rice. Plant Cell, 2005, 17: 3311–3325

[20] Sheehan M J, Farmer P R, Brutnell T P. Structure and expression of maize phytochrome family homeologs. Genetics, 2004, 167: 1395–1405

[21] Kern R, Gasch A, Deak M, Kay S A, Chua N H. PhyB of tobacco, a new member of the phytochrome family. Plant Physiol, 1993, 102: 1363–1364

[22] Childs K L, Miller F R, Cordonnier-Pratt M M, Pratt L H, Morgan P W, Mullet J E. The sorghum photoperiod sensitivity gene, Ma3, encodes a phytochrome B. Plant Physiol, 1997, 113: 611–619

[23] Tuskan G A, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, et al. The genome of black cottonwood, Populus trichocarpa (Torr. & Gray). Science, 2006, 313: 1596–1604

[24] Sz?cs P, Karsai I, von Zitzewitz J, Mészárps K, Cooper L L D, Gu Y Q, Chen T H H, Hayes P M, Skinner J S. Positional relationships between photoperiod response QTL and photoreceptor and vernalization genes in barley. Theor Appl Genet, 2006, 112: 1277–1285

[25] Janda J, Bartoš J, Šafá? J, Kubaláková M, Valárik M, ?íhalíková J, Šimková H, Caboche M, Sourdille P, Bernard M, Chalhoub B, Dole?el J. Construction of a subgenomic BAC library specific for chromosomes 1D, 4D, and 6D of hexaploid wheat. Theor Appl Genet, 2004, 109: 1337–1345

[26] Sheehan M J, Kennedy L M, Costich D E, Brutnell T P. Subfunctionalization of PhyB1 and PhyB2 in the control of seedling and mature plant traits in maize. Plant J, 2007, 49: 338–353

[27] Boccalandro H E, Rugnone M L, Moreno J E, Ploschuk E L, Serna L, Yanovsky M J, Casal J J. Phytochrome B enhances photosynthesis at the expense of water-use efficiency in Arabidopsis. Plant Physiol, 2009, 150: 1083–1092
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