Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (12): 2045-2054.doi: 10.3724/SP.J.1006.2010.02045

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Mapping QTLs for Male Sterile Gene in YM-Type Thermo-Sensitive Male Sterile Line of Wheat

ZHOU Ju-Hong1,LI Ke1,HE Bei-Ru1,HU Yin-Gang1,2,3,*   

  1. 1 College of Agronomy, Northwest A&F University, Yangling 712100, China; 2 Yangling Branch of China National Wheat Improvement Center,  Yangling 712100, China; 3 Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, China
  • Received:2010-04-12 Revised:2010-07-28 Online:2010-12-12 Published:2010-10-14
  • Contact: 胡银岗,E-mail:huyingang@yahoo.com.cn

PDF

1868

Cited

5

Abstract: The sterile gene of YM-type thermo-sensitive male sterile wheat (Triticum aestivum L.) line has been mapped on 1Bs chromosome with genetic distance to adjacent molecular markers more than 10 cM. To further locate this thermo-sensitive male sterile gene, we constructed a mapping population with 200 F2 plants from the cross between ATM3314 and restorer line Chinese Spring. Twenty-two simple sequence repeat (SSR) markers distributing evenly on 1Bs were screened between the parents and the male sterile and fertile bulks, and five markers showed polymorphism. These markers were then tested in the F2 population. A 1Bs partial linkage map with the five SSR markers was obtained and QTLs for the male sterility were detected using composite interval mapping method. One major QTL and one minor QTL were detected, which were designated as rfv1-1 and rfv1-2, respectively. QTL rfv1-1 was located between the SSR markers Xgwm18 and Xwmc406 on chromosome 1Bs with genetic distances of 6.0 cM and 4.6 cM, respectively. The LOD value for this locus was 8.80, and the gene effects were 23.87 for additive effect and 10.44 for dominant effect. This QTL explained 23.91% of the phenotypic variation. QTL rfv1-2 was mapped between markers Xwmc406and Xbarc8 with genetic distances of 4.0 cM and 3.4 cM, respectively. The LOD value of this QTL was 3.10. This locus had additive effect of 17.59 and dominant effect of 5.99, and explained 7.78% of the phenotypic variation. These results are propitious for fine mapping and positional cloning of this male sterile gene.

Key words: Wheat, Thermo-sensitive male sterile gene, SSR maker, QTL mapping

[1]Cao S-H(曹双河), Guo X-L(郭小丽), Liu D-C(刘冬成), Zhang X-Q(张相岐), Zhang A-M(张爱民). Preliminary gene-mapping of photoperiod-temperature sensitive genic male sterility in wheat (Triticum aestivum L.). Acta Genet Sin (遗传学报), 2004, 31(3): 293-298 (in Chinese with English abstract)
[2]Ma L-J(马翎健), He B-R(何蓓如), Song X-Y(宋喜悦), Hu Y-G(胡银岗). Heredity and RAPD markers analysis of wheat photoperiod-sensitive male sterile gene. Acta Agron Sin (作物学报), 2004, 30(9): 912-915 (in Chinese with English abstract)
[3]Jiang H-M(江红梅), Zhang L-P(张立平). Advanced heredity research on photoperiod-temperature male sterility in wheat. Seed (种子), 2009, (5): 56-59 (in Chinese)
[4]Kihara H. Substitution of nucleus and its effects on genome manifestations. Cytologia, 1951, 16: 177-193
[5]Sasakuma T, Ohtsuka L. Cytoplasmic effects of Aegilops species having D genome in wheat: I. Cytoplasmic differentiation among five species regarding pistillody induction. Seiken Ziho, 1979, 27-28, 59-65
[6]He J-M(何觉民), Dai J-T(戴君惕), Zou Y-B(邹应斌), Zhou M-L(周美兰), Zhang H-Q(张海青), Liu X-L(刘雄伦). Studies on two-line hybrid wheat: I. Discovery, development and utilization potential of male sterile wheat based on ecological environments. Hunan Agric Sci (湖南农业科学), 1992, (5): 1-3 (in Chinese)
[7]Tan C-H(谭昌华), Yu G-Q(余国东), Yang P-F(杨沛丰), Zhang Z-H(张宗华), Pan Y(潘鹰), Zheng J(郑坚). Preliminary study on sterility of thermo-photoperiod-sensitive male sterile wheat in Chongqing. Southwest China J Agric Sci (西南农业学报), 1992, 5(1): 1-4 (in Chinese with English abstract)
[8]Fu D-X(傅大雄), Ruan R-W(阮仁武). Discovery of photoperiod and temperature sensitive character of KM-type male sterile wheat lines and breeding of two-line hybrid wheat. Southwest China J Agric Sci (西南农业学报), 1993, 6(1): 117-118 (in Chinese)
[9]Zhao C-P(赵昌平), Wang X(王新), Zhang F-Y(张风延), Ye Z-J(叶志杰), Dai H-J(戴惠君). Progress of hybrid wheat research and developing hybrid wheat using thermo-sensitive male sterile lines. Beijing Agric Sci (北京农业科学), 1999, 17(2): 3-5 (in Chinese)
[10]Rong D-F(荣德福), Cao W-M(曹卫民). The types of photoperiod-thermo-sensitive male sterile wheat and breeding for long- day and high-temperature sensitive male sterile wheat line. Triticeae Crops (麦类作物), 1999, 19(1): 20-24 (in Chinese)
[11]He B-R(何蓓如). A breeding method for breeding thermo-sensi- tive male sterile wheat line adapted to Huang-Huai region of China. Chinese patent: ZL00105488.0. 2004-03-10 (in Chinese)
[12]Ma Z Q, Sorrells M E. Genetic analysis of fertility restoration in wheat using restriction fragment length polymorphisms. Crop Sci, 1995, 35: 1137-1143
[13]Kojima T, Tsujimoto H, Ogihara Y. High resolution RFLP mapping of the fertility restoration (Rf3) gene against Triticum timopheevi cytoplasm located on chromosome 1BS of common wheat. Genes Genet Syt, 1997, 72: 353-359
[14]Guan R-X(关荣霞), Guo X-L(郭小丽), Liu D-C(刘冬成), Cao S-H(曹双河), Zhang A-M(张爱民). Analysis of the fertility restoring gene Rf6 in T. timopheevii cytoplasimic male sterility of wheat with ISSR markers. Sci Agric Sin (中国农业科学), 2002, 35(11): 1297-1301 (in Chinese with English abstract)
[15]Zhang C(张萃), Wang H-Y(王宏英), Shen Y-Z(沈银柱), Zhao B-C(赵宝存), Zhu Z-G(朱正歌), Huang Z-J(黄占景). Location of the fertility restorer gene for T-type CMS wheat by microsatellite marker. Acta Genet Sin (遗传学报), 2003, 30(5): 295-264 (in Chinese with English abstract)
[16]Liu B-S(刘保申), Sun Q-X(孙其信), Gao Q-R(高庆荣), Sun L-Z(孙兰珍), Xie C-J(解超杰), Li C-Y(李传友), Ni Z-F(倪中福), Dou B-D(窦秉德), Wei Y-L(魏艳玲). Mapping of fertility restoring gene for Aegilops Kotschy cytoplasmic male sterility in wheat using SSR markers. Sci Agric Sin (中国农业科学), 2002 , 35(4) : 354-358 (in Chinese with English abstract)
[17]Liu B S, Sun Q X, Sun L Z, Gao Q R, Xie C J, Dou B-D, Ni Z F, Wei Y L, Zhang Y C. RAPD and ISSR markers of fertility restoring gene for Aegilops Kotschyi cytoplasmic male sterility in wheat. Acta Bot Sin, 2002, 44: 446-450
[18]Shi Y-Q(石运庆), Mou Q-H(牟秋焕), Li P(李鹏), Liu B-S(刘保申). Mapping of fertility restoring gene for Aegilops ventericosa cytoplasmic male sterility in wheat using SSR markers. Shandong Agric Sci (山东农业科学), 2005, (3): 3-5 (in Chinese with English abstract)
[19]Xing Q H, Ru Z G, Zhou C J, Xue X, Liang G Y, Yang D E. Genetic analysis, molecular tagging and mapping of the thermo- sensitive genic male sterile gene (wtmsl) in wheat. Theor Appl Genet, 2003, 107: 1500-1504
[20]He B-R(何蓓如), Hu Y-G(胡银岗), Song X-Y(宋喜悦), Ma L-J(马翎健), Li H-B(李宏斌), Dong P-H(董普辉), Yu L(于玲). Preliminary study on the fertility conversion of thermo-sensitive male sterile wheat line YM3314 with the chromosome segments of T. macha. J Triticeae Crops (麦类作物学报), 2008, 28(2): 206-209 (in Chinese with English abstract)
[21]Dong P-H(董普辉). Study on the Chromosomal Location of the T-type Restorer Gene and Linkage Analysis between It and the K- type Male Sterile Gene in T. macha. PhD Dissertation of Northwest A&F University, 2005 (in Chinese with English abstract)
[22]Dong P-H(董普辉), He B-R(何蓓如), Song X-Y(宋喜悦), Hu Y-G(胡银岗), Ma L-J(马翎健), Yu L(于玲), Li H-B(李宏斌). Chromosomal location of T-type fertility restoring gene derived from T. macha var. subletschchumicum. J Triticeae Crops (麦类作物学报), 2006, 26(1): 13-16 (in Chinese with English abstract)
[23]Dong P-H(董普辉), Hu Y-G(胡银岗), Lin F-Y(林凡云), Song G-Q(宋国琦), Song X-Y(宋喜悦), Ma L-J(马翎健), Li H-B(李宏斌), He B-R(何蓓如). SSR markers analysis of T-type restorer gene Rf3 and K-type male sterile gene rfv1 derived from Triticum macha L. J Triticeae Crops (麦类作物学报), 2009, 29(5): 766-769 (in Chinese with English abstract)
[24]Aldrich C. CTAB DNA extraction from plant tissues. Plant Mol Biol Rep, 1993, 11: 128-141
[25]Somers D J, Isaac P, Edwards K. A high-density wheat micro satellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet, 2004, 109: 1105-1114
[26]Li H-Y(李会勇), Wang T-Y(王天宇), Li Y(黎裕), Shi Y-S(石云素), Song Y-C(宋燕春), Lu P(陆平). Application of the TP-M13 automated fluorescent-labeled system of SSR genotyping in sorghum. J Plant Genet Resour (植物遗传资源学报), 2005, 6(1): 68-70 (in Chinese with English abstract)
[27]Liu Z-Z(刘志斋), Wang T-Y(王天宇), Li Y(黎裕). TP-M13-SSR technique and its applications in the analysis of genetic diversity in maize. J Maize Sci (玉米科学), 2007, 15(6): 10-15 (in Chinese with English abstract)
[28]Lincoln S E, Daly M J, Lander E S. Constructing Genetic Linkage Maps with MAPMAKER/EXP Version 3.0: a tutorial and reference manual. In: A Whitehead Institute for Biomedical Research Technical Report, 3rd edn. Cambridge, USA: Whitehead Institute for Biomedical Research, 1993
[29]Liu R-H(刘仁虎), Meng J-L(孟金陵). Map Draw: a Microsoft Excel macro for drawing genetic linkage maps based on given genetic linkage data. Hereditas (遗传), 2003, 25(3): 317-321 (in Chinese with English abstract)
[30]Wang S, Basten C J, Zeng Z B. Windows QTL. Cartographer 2.5 Department of Statistics. Carolina: North Carolina State University, 2006
[31]Cao G, Zhu J, He C, Gao Y, Yan J, Wu P. Impact of epitasis and QTL × environment interaction on the developmental behavior of plant height in rice (Oryza sativa L.). Theor Appl Genet, 2001, 103: 153-160
[32]Tian Z-M(田再民), Zhang L-P(张立平), Sun Q-L(孙庆林), Shan F-H(单福华), Tian Z-H(田自华). Methodology and advances in QTL of significant agronomic traits in wheat. J Inner Mongolia Agric Univ (Nat Sci Edn)(内蒙古农业大学学报·自然科学版), 2007, 28(4): 243-247 (in Chinese with English abstract)
[33]Song X-Y(宋喜悦), Dong P-H(董普辉), Hu Y-G(胡银岗), Ma L-J(马翎健), Li H-B(李宏斌), He B-R(何蓓如). Linkage relationship between T-type restorer gene Rf-3 and K-type male ste- rile gene rfv-1 in Triticum macha L. Acta Agron Sin (作物学报), 2008, 34(10): 1757-1761 (in Chinese with English abstract)
[34]Gu H(顾慧), Qi C-K(戚存扣). QTL analysis of lodging resistance in Brassica napus L. Jiangsu J Agric Sci (江苏农业学报), 2009, 25(3): 484-489 (in Chinese with English abstract)
[35]Liang F-S(梁凤山), Wang B(王斌). Heredity and gene mapping of male sterility in wheat. Hereditas (遗传), 2003, 25(4): 461- 465 (in Chinese with English abstract)
[36]Hu Y-G(胡银岗), Ma L-J(马翎健), Song X-Y(宋喜悦), Fang P(方鹏), He B-R(何蓓如), Zhang W-J(张文俊). Molecular markers of the fertility genes Rf-3 and rfk-1 in the 1BS chromosome of T. spelta. J Northwest Sci-Tech Univ Agric For (Nat Sci Edn)(西北农林科技大学·自然科学版), 2004, 32(1): 56-62 (in Chinese with English abstract)
[37]Guo R-X(郭瑞星). Genetic Analysis and Molecular Mapping of the Thermo-Photoperiod Sensitive Genic Male Sterile Genes in Wheat Male Sterile Line 337S (Triticum aestivum L.). PhD Dissertation of Huazhong Agricultural University, 2005 (in Chinese with English abstract)
[38]Guo R X, Dong D F, Tan Z B, Rong D F, Li C D. Two recessive genes controlling thermophotoperiod-sensitive male sterility in wheat. Theor Appl Genet, 2006, 112: 1271-1276
[39]Chi H-F(池慧芳). QTL Mapping for Photoperiod-Thermo Sensitive Genic Male Sterility and Red-auricle in Wheat. MS Dissertation of Inner Mongolia Agricultural University, 2008 (in Chinese with English abstract)
[1] HU Wen-Jing, LI Dong-Sheng, YI Xin, ZHANG Chun-Mei, ZHANG Yong. Molecular mapping and validation of quantitative trait loci for spike-related traits and plant height in wheat [J]. Acta Agronomica Sinica, 2022, 48(6): 1346-1356.
[2] GUO Xing-Yu, LIU Peng-Zhao, WANG Rui, WANG Xiao-Li, LI Jun. Response of winter wheat yield, nitrogen use efficiency and soil nitrogen balance to rainfall types and nitrogen application rate in dryland [J]. Acta Agronomica Sinica, 2022, 48(5): 1262-1272.
[3] LEI Xin-Hui, WAN Chen-Xi, TAO Jin-Cai, LENG Jia-Jun, WU Yi-Xin, WANG Jia-Le, WANG Peng-Ke, YANG Qing-Hua, FENG Bai-Li, GAO Jin-Feng. Effects of soaking seeds with MT and EBR on germination and seedling growth in buckwheat under salt stress [J]. Acta Agronomica Sinica, 2022, 48(5): 1210-1221.
[4] FU Mei-Yu, XIONG Hong-Chun, ZHOU Chun-Yun, GUO Hui-Jun, XIE Yong-Dun, ZHAO Lin-Shu, GU Jia-Yu, ZHAO Shi-Rong, DING Yu-Ping, XU Yan-Hao, LIU Lu-Xiang. Genetic analysis of wheat dwarf mutant je0098 and molecular mapping of dwarfing gene [J]. Acta Agronomica Sinica, 2022, 48(3): 580-589.
[5] FENG Jian-Chao, XU Bei-Ming, JIANG Xue-Li, HU Hai-Zhou, MA Ying, WANG Chen-Yang, WANG Yong-Hua, MA Dong-Yun. Distribution of phenolic compounds and antioxidant activities in layered grinding wheat flour and the regulation effect of nitrogen fertilizer application [J]. Acta Agronomica Sinica, 2022, 48(3): 704-715.
[6] LIU Yun-Jing, ZHENG Fei-Na, ZHANG Xiu, CHU Jin-Peng, YU Hai-Tao, DAI Xing-Long, HE Ming-Rong. Effects of wide range sowing on grain yield, quality, and nitrogen use of strong gluten wheat [J]. Acta Agronomica Sinica, 2022, 48(3): 716-725.
[7] XU Long-Long, YIN Wen, HU Fa-Long, FAN Hong, FAN Zhi-Long, ZHAO Cai, YU Ai-Zhong, CHAI Qiang. Effect of water and nitrogen reduction on main photosynthetic physiological parameters of film-mulched maize no-tillage rotation wheat [J]. Acta Agronomica Sinica, 2022, 48(2): 437-447.
[8] YAN Yan, ZHANG Yu-Shi, LIU Chu-Rong, REN Dan-Yang, LIU Hong-Run, LIU Xue-Qing, ZHANG Ming-Cai, LI Zhao-Hu. Variety matching and resource use efficiency of the winter wheat-summer maize “double late” cropping system [J]. Acta Agronomica Sinica, 2022, 48(2): 423-436.
[9] WANG Yang-Yang, HE Li, REN De-Chao, DUAN Jian-Zhao, HU Xin, LIU Wan-Dai, GU Tian-Cai, WANG Yong-Hua, FENG Wei. Evaluations of winter wheat late frost damage under different water based on principal component-cluster analysis [J]. Acta Agronomica Sinica, 2022, 48(2): 448-462.
[10] CHEN Xin-Yi, SONG Yu-Hang, ZHANG Meng-Han, LI Xiao-Yan, LI Hua, WANG Yue-Xia, QI Xue-Li. Effects of water deficit on physiology and biochemistry of seedlings of different wheat varieties and the alleviation effect of exogenous application of 5-aminolevulinic acid [J]. Acta Agronomica Sinica, 2022, 48(2): 478-487.
[11] MA Bo-Wen, LI Qing, CAI Jian, ZHOU Qin, HUANG Mei, DAI Ting-Bo, WANG Xiao, JIANG Dong. Physiological mechanisms of pre-anthesis waterlogging priming on waterlogging stress tolerance under post-anthesis in wheat [J]. Acta Agronomica Sinica, 2022, 48(1): 151-164.
[12] MENG Ying, XING Lei-Lei, CAO Xiao-Hong, GUO Guang-Yan, CHAI Jian-Fang, BEI Cai-Li. Cloning of Ta4CL1 and its function in promoting plant growth and lignin deposition in transgenic Arabidopsis plants [J]. Acta Agronomica Sinica, 2022, 48(1): 63-75.
[13] WEI Yi-Hao, YU Mei-Qin, ZHANG Xiao-Jiao, WANG Lu-Lu, ZHANG Zhi-Yong, MA Xin-Ming, LI Hui-Qing, WANG Xiao-Chun. Alternative splicing analysis of wheat glutamine synthase genes [J]. Acta Agronomica Sinica, 2022, 48(1): 40-47.
[14] LI Ling-Hong, ZHANG Zhe, CHEN Yong-Ming, YOU Ming-Shan, NI Zhong-Fu, XING Jie-Wen. Transcriptome profiling of glossy1 mutant with glossy glume in common wheat (Triticum aestivum L.) [J]. Acta Agronomica Sinica, 2022, 48(1): 48-62.
[15] LUO Jiang-Tao, ZHENG Jian-Min, PU Zong-Jun, FAN Chao-Lan, LIU Deng-Cai, HAO Ming. Chromosome transmission in hybrids between tetraploid and hexaploid wheat [J]. Acta Agronomica Sinica, 2021, 47(8): 1427-1436.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd