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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (09): 1505-1510.doi: 10.3724/SP.J.1006.2011.01505

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Genetic Stability of Rice Aneuploid during Its Asexual Propagation

GONG Zhi-Yun,SHI Guo-Xin,LIU Xiu-Xiu,YI Chuan-Deng,YU Heng-Xiu*   

  1. Key Laboratory of Crop Genetics and Physiology of Jiangsu Province / Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, China
  • Received:2011-01-12 Revised:2011-05-20 Online:2011-09-12 Published:2011-06-28
  • Contact: 于恒秀, E-mail: hxyu@yzu.edu.cn, ?Tel: 0514-87979304, Fax: 0514-87972138

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Abstract: Telotetrasome is a kind of aneuploid with two additional identical telocentric chromosomes. To investigate the genetic stability of riceaneuploid during its asexual propagation,a telotetrasome (2n+·8S+·8S) was selected from the progenies of a rice telotrisome (2n+·8S), and preserved by asexsual reproduction. But one of the extra short arms (·8S) was easy to be lost in the asexual propagation offspring of 2n+·8S+·8S and led to morphological variations. FISH results indicated that one of the two extra ·8S was short of detectable rice centromeric satellite repeat (CentO) and centromere-specific retrotransposon (CRR) and could not be transmitted stably. The other extra ·8S contained CRR, but not detectable CentO and could be transmitted steadily. However, the extra ·8S contained the CentO and CRR sequences simultaneously in the initial telotrisomic line (2n+·8S). These results showed that CentO and CRR of the extra ·8S may be randomly lost and led inheritance instability in the aneuploid harbouring extra ·8S during asexual propagation.

Key words: Rice, Aneuploid, Telocentric chromosome, CentO, CRR

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