Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (02): 230-237.doi: 10.3724/SP.J.1006.2013.00230

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Isolation and Functional Analysis of a New DREB Transcription Factor (BpDREB1) from Brassica pekinesis

LIU Xiao-Ying,CHEN Li-Yuan,ZHANG Jing-Qiu,LI Jia-Wei,GAO Yue,WANG Zhen-Ying*   

  1. Tianjin Key Laboratory of Cytogenetical and Molecular Regulation / College of Life Sciences, Tianjin Normal University, Tianjin 300387, China
  • Received:2012-05-10 Revised:2012-10-09 Online:2013-02-12 Published:2012-11-14
  • Contact: 王振英, E-mail: wzycell@yahoo.com.cn

Abstract:

DREB1/CBF transcription factors play an important role in plant stress tolerance, and one of important significance to gain stress-tolerant crops by transgenic technologies. In this study, a DREB-like gene, named BpDREB1 (accession No. EF219470), was cloned from Chinese cabbage. The BpDREB1 cDNA was 647 bp in length, and encoded protein of 213 amino acids with an predicted molecular weight of 23 kDand a isoelectric point of5.11, and shared 94% similarity with other DREB transcription factor from Chinese cabbage. On the basis of multiple sequence alignment and phylogenetic analysis, BpDREB1 was classified in A-1 group of the DREB family. The expression patterns analysis indicated that BpDREB1 was strongly up-regulated at low temperature, also responded to dehydrationHowever, the expression of BpDREB1 was not affected by high salinity. The expression pattern of BpDREB1 was the same as that of other DREB transcription factors in A-1 group. Overexpression of BpDREB1 greatlyincreased the contents of total soluable sugar andfree prolinein transgenic Arabidopsis plants, demonstrating that transgenic Arabidopsis induced the expression of soluable sugar and prolin related genes to enhance the tolerance to stress. These results suggest that BpDREB1 from Chinese cabbage has the typical characteristic of DREB transcription factors, and functions under the stress conditions including low temperature and drought.

Key words: Chinese cabbage, BpDREB1, Low temperature, Dehydration

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