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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (03): 381-389.doi: 10.3724/SP.J.1006.2014.00381

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Cloning and Analysis of 5′ Flanking Regions of Arachisis hypogaea L. Genes Encoding Plastidial Acyl Carrier Protein

SHAN Lei1,2,*,TANG Gui-Ying1,XU Ping-Li1,ZHAO Xue-Bin1,3,LIU Zhan-Ji1   

  1. 1 Hi-Tech Research Centre, Shandong Academy of Agricultural Sciences/Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan 250100, China; 2 College of Agriculture, Shandong University, Jinan 250100, China; 3 College of Life Science, Shandong Normal University, Jinan 250014, China
  • Received:2013-07-03 Revised:2013-10-31 Online:2014-03-12 Published:2014-01-16
  • Contact: 单雷, E-mail: shlei1025@sina.com, Tel: 0531-83179435

Abstract:

We cloned the 5′ flanking fragments of three peanut plastidial acyl carrier protein (ACP) genes AhACP1, AhACP4,and AhACP5 by chromosome walking method, and the fragments sizes were 535, 1400, and 1180 bp, respectively. Using 5′ RACE (5′ Rapid Amplification of cDNA End), the transcription start sites of the three genes were localized on 71, 92, and 71 bp from the translation initiation codon ATG, respectively. The crucial regulation elements in promoters of three peanut ACP genes and four Arabidopsis ACP genes were further analyzed with softwares PLACE and Plant CARE (http://www.dna.affrc.go.jp/PLACE; http://bioinformatics.psb.ugebp.be/webtools/plabpcare/html), showing that although AhACP4 and AhACP5 had similar expression patterns in root, stem, leaf, flower and seed at different development stages, their promoter regions contained their specific cis-elements. For instance, the promoter region of AhACP4 consisted of the regulation element WUS expressingin meristems of root or shoot primordia while that of AhACP5 had several key regulation elements such as E2FB, TELO BOX, and UP1 required in sprouting and expansion of axillary shoots, suggesting that their expression patterns may be various in different tissues and development stages. This study also indicated that the expression profiles of orthologous genes peanut AhACP4 and Arabidopsis AtACP4 diverged in evolution, and the AhACP4 gene expressed in a constitutive pattern while the AtACP4 expressed mainly in leaf, and in comparison with the promoter region of AtACP4, that of AhACP4 contained fewer light regulation elements.

Key words:  Plastidial acyl carrier protein (ACP), Promoter, Regulation element, Peanut (Arachis hypogaea L.)

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