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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (04): 571-580.doi: 10.3724/SP.J.1006.2014.00571

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS •     Next Articles

Transcriptional and Translational Characterization of Rice Chitinase Genes

FAN Wei**,LI Xue-Jiao**,GUAN Ming-Li,MIAO Liu-Yang,SHI Jia-Nan,DOU Shi-Juan,LIU Li-Juan,LI Li-Yun,LIU Guo-Zhen*   

  1. College of Life Sciences, Agricultural University of Hebei, Baoding 071000, China
  • Received:2013-08-08 Revised:2014-01-12 Online:2014-04-12 Published:2014-02-14
  • Contact: 刘国振, E-mail: gzhliu@genomics.org.cn, Tel: 0312-7528250

Abstract:

Plant chitinases play roles in plant development and stress responses. Nineteen chitinase genes, belonging to pathogenesis related (PR) 3 family, were found in rice genome. In this study, constitutive and tissue-specific transcripted chitinase genes were identified, and the domain architecture of chitinase protein was predicted. Clustering analysis and subfamily classification were also carried out. Furthermore, chitinase protein expression profiling was surveyed using western blot (WB), it was found that the expression of CHIT5 was down-regulated in normal growth rice leaves, while the expressions of CHIT6, CHIT14, CHITC1, and CHITC2 were up-regulated. In the incompatible interaction between rice and Xanthomonas oryzae pv. oryzae (Xoo), the expressions of CHIT1, CHIT2, CHIT5, CHIT6, CHIT10, CHIT15, and CHIT16 were enhanced after inoculation and three of CHIT14, CHITC1 and CHITC2 down-regulated. In addition, the comparison of chitinase protein expression in different rice-Xoo interactions showed that these were similar alternation patterns between compatible and incompatible reactions, with a higher extent of alterations in incompatible interactions for most of chitinases. It is interesting to note that the expression of CHIT6 was enhanced in mock-treated samples, suggesting that the expression of CHIT6 may be induced by a mechanic wound. The data revealed in this research will provide useful clues for the understanding of the function of PR3 family chitinase genes.

Key words: Rice, Chitinase, Clustering analysis, Xanthomonas oryzae pv. oryzae (Xoo), Transcription, Western blot

[1]Boller T. Chemoperception of microbial signals in plant cells. Annu Rev Plant Biol, 1995, 46: 189–214

[2]Liu T, Liu Z, Song C, Hu Y, Han Z, She J, Fan F, Wang J, Jin C, Chang J, Zhou J M, Chai J. Chitin-induced dimerization activates a plant immune receptor. Science, 2012, 336: 1160–1164

[3]Kaku H, Nishizawa Y, Ishii-Minami N, Akimoto-Tomiyama C, Dohmae N, Takio K, Minami E, Shibuya N. Plant cells recognize chitin fragments for defense signaling through a plasma membrane receptor. Proc Natl Acad Sci USA, 2006, 103: 11086–11091

[4]Shimizu T, Nakano T, Takamizawa D, Desaki Y, Ishii-Minami N, Nishizawa Y, Minami E, Okada K, Yamane H, Kaku H, Shibuya N. Two LysM receptor molecules, CEBiP and OsCERK1, cooperatively regulate chitin elicitor signaling in rice. Plant J, 2010, 64: 204–214

[5]Kishimoto K, Kouzai Y, Kaku H, Shibuya N, Minami E, Nishizawa Y. Perception of the chitin oligosaccharides contributes to disease resistance to blast fungus Magnaporthe oryzae in rice. Plant J, 2010, 64: 343–354

[6]Kouzai Y, Kaku H, Shibuya N, Minami E, Nishizawa Y. Expression of the chimeric receptor between the chitin elicitor receptor CEBiP and the receptor-like protein kinase Pi-d2 leads to enhanced responses to the chitin elicitor and disease resistance against Magnaporthe oryzae in rice. Plant Mol Biol, 2013, 81: 287–295

[7]Kumar S, Sharma R, Tewari R. Production of N-acetylglucosamine using recombinant chitinolytic enzymes. Indian J Microbiol, 2011, 51: 319–325

[8]Kitajima S, Sato F. Plant pathogenesis-related proteins: molecular mechanisms of gene expression and protein function. J Biochem, 1999, 125: 1–8

[9]Nakazaki T, Tsukiyama T, Okumoto Y, Kageyama D, Naito K, Inouye K, Tanisaka T. Distribution, structure, organ-specific expression, and phylogenic analysis of the pathogenesis-related protein-3 chitinase gene family in rice (Oryza sativa L.). Genome, 2006, 49: 619–630

[10]de A G L B, Sachetto-Martins G, Contarini M G, Sandroni M, de P F R, de Lima V M, Cordeiro M C, de Oliveira D E, Margis-Pinheiro M. Arabidopsis thaliana class IV chitinase is early induced during the interaction with Xanthomonas campestris. FEBS Lett, 1997, 419: 69–75

[11]Samac D A, Shah D M. Developmental and pathogen-induced activation of the Arabidopsis acidic chitinase promoter. Plant Cell, 1991, 3: 1063–1072

[12]Nielsen K K, Bojsen K, Roepstorff P, Mikkelsen J D. A hydroxyproline-containing class IV chitinase of sugar beet is glycosylated with xylose. Plant Mol Biol, 1994, 25: 241–257

[13]Park C H, Kim S, Park J Y, Ahn I P, Jwa N S, Im K H, Lee Y H. Molecular characterization of a pathogenesis-related protein 8 gene encoding a class III chitinase in rice. Mol Cells, 2004, 17: 144–150

[14]Neale A D, Wahleithner J A, Lund M, Bonnett H T, Kelly A, Meeks-Wagner D R, Peacock W J, Dennis E S. Chitinase, beta-1,3-glucanase, osmotin, and extensin are expressed in tobacco explants during flower formation. Plant Cell, 1990, 2: 673–684

[15]Robinson S P, Jacobs A K, Dry I B. A class IV chitinase is highly expressed in grape berries during ripening. Plant Physiol, 1997, 114: 771–778

[16]Passarinho P A, Van Hengel A J, Fransz P F, de Vries S C. Expression pattern of the Arabidopsis thaliana AtEP3/AtchitIV endochitinase gene. Planta, 2001, 212: 556–567

[17]Zhong R, Kays S J, Schroeder B P, Ye Z H. Mutation of a chitinase-like gene causes ectopic deposition of lignin, aberrant cell shapes, and overproduction of ethylene. Plant Cell, 2002, 14: 165–179

[18]Wu B, Zhang B, Dai Y, Zhang L, Shang-Guan K, Peng Y, Zhou Y, Zhu Z. Brittle culm15 encodes a membrane-associated chitinase-like protein required for cellulose biosynthesis in rice. Plant Physiol, 2012, 159: 1440–1452

[19]Datta K, Tu J, Oliva N, Ona I I, Velazhahan R, Mew T W, Muthukrishnan S, Datta S K. Enhanced resistance to sheath blight by constitutive expression of infection-related rice chitinase in transgenic elite indica rice cultivars. Plant Sci, 2001, 160: 405–414

[20]Nishizawa Y, Nishio Z, Nakazono K, Soma M, Nakajima E, Ugaki M, Hibi T. Enhanced resistance to blast (Magnaporthe grisea) in transgenic Japonica rice by constitutive expression of rice chitinase. Theor Appl Genet, 1999, 99: 383–390

[21]Snelling J. Role of Oryza sativa Chitinases in Disease Resistance. MS Thesis of Colorado State University, 2005

[22]Kovacs G, Sagi L, Jacon G, Arinaitwe G, Busogoro J P, Thiry E, Strosse H, Swennen R, Remy S. Expression of a rice chitinase gene in transgenic banana (‘Gros Michel’, AAA genome group) confers resistance to black leaf streak disease. Transgenic Res, 2013, 22: 117–130

[23]Huang X, Wang J, Du Z, Zhang C, Li L, Xu Z. Enhanced resistance to stripe rust disease in transgenic wheat expressing the rice chitinase gene RC24. Transgenic Res, 2013, 22: 939–947

[24]Bai H, Lan J P, Gan Q, Wang X Y, Hou M M, Cao Y H, Li L Y, Liu L J, Hao Y J, Yin C C, Wu L, Zhu L H, Liu G Z. Identification and expression analysis of components involved in rice Xa21-mediated disease resistance signalling. Plant Biol (Stuttg), 2012, 14: 914–922

[25]Wang Y, Pi L, Chen X, Chakrabarty P K, Jiang J, De Leon A L, Liu G-Z, Li L, Benny U, Oard J. Rice XA21 binding protein 3 is a ubiquitin ligase required for full Xa21-mediated disease resistance. Plant Cell Online, 2006, 18: 3635–3646

[26]Park C J, Peng Y, Chen X, Dardick C, Ruan D, Bart R, Canlas P E, Ronald P C. Rice XB15, a protein phosphatase 2C, negatively regulates cell death and XA21-mediated innate immunity. PLoS Biol, 2008, 6(9): e231

[27]Chen X, Chern M, Canlas P E, Ruan D, Jiang C, Ronald P C. An ATPase promotes autophosphorylation of the pattern recognition receptor XA21 and inhibits XA21-mediated immunity. Sci Signal, 2010, 107: 8029

[28]Park C J, Bart R, Chern M, Canlas P E, Bai W, Ronald P C. Overexpression of the endoplasmic reticulum chaperone BiP3 regulates XA21-mediated innate immunity in rice. PLoS ONE, 2010, 5(2): e9262

[29]Wu Q, Hou M, Li L, Liu L, Hou Y, Liu G. Induction of pathogenesis-related proteins in rice bacterial blight resistant gene XA21-mediated interactions with Xanthomonas oryzae pv. oryzae. J Plant Pathol, 2011, 93: 455–459

[30]Hou M, Xu W, Bai H, Liu Y, Li L, Liu L, Liu B, Liu G. Characteristic expression of rice pathogenesis-related proteins in rice leaves during interactions with Xanthomonas oryzae pv. oryzae. Plant Cell Rep, 2012, 31: 895–904

[31]缪刘杨, 李莉云, 刘钊, 刘雨萌, 江光怀, 杨凤环, 何晨阳, 刘国振. 五个WRKY转录因子在水稻叶片生长和抗病反应中的表达研究. 生物化学与生物物理进展, 2013, 40: 356–364

Miao L Y, Li L Y, Liu Z, Liu Y M, Jiang G H, Yang F H, He C Y, Liu G Z, Jiang G H. Characteristic expression analysis of five WRKY transcriptional factors in rice leaf growth and disease resistance reaction. Prog Biochem Biophys, 2013, 40: 356–364 (in Chinese with English abstract)

[32]史佳楠, 李莉云, 徐文静, 关明俐, 李雪姣, 牛东东, 兰金苹, 窦世娟, 刘丽娟, 刘国振. 八个WRKY转录因子在水稻叶片生长和抗病过程中的表达研究. 植物病理学报, 2014(已接受)

Shi J N, Li L Y, Xu W J, Guan M L, Li X J, Niu D D, Lan J P, Dou S J, Liu L J, Liu G Z. Expression analysis of eight WRKY transcription factors in rice leaf growth and disease resistance response. Acta Phytopathol Sin, 2014 (accepted) (in Chinese with English abstract)

[33]窦世娟, 关明俐, 李莉云, 刘国振. 水稻的病程相关基因. 科学通报, 2013, DOI:10.1360/972012-1831

Dou S J, Guan M L, Li L Y, Liu G Z. The pathogenesis-related genes of rice. Chin Sci Bull, 2013, DOI:10.1360/972012-1831 (in Chinese with English abstract)

[34]Li X, Bai H, Wang X, Li L, Cao Y, Wei J, Liu Y, Liu L, Gong X, Wu L. Identification and validation of rice reference proteins for western blotting. J Exp Bot, 2011, 62: 4763–4772

[35]刘国振, 刘斯奇, 吴琳, 徐宁志. 基于抗体的水稻蛋白质组学: 开端与展望. 中国科学: 生命科学, 2011, 41: 173–177

Liu G Z, Liu S Q, Wu L, Xu N Z. Antibody-based rice proteomics: the beginning and perspectives. Sci Sin Vitae, 2011, 41: 173–177 (in Chinese with English abstract)
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