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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (04): 571-580.doi: 10.3724/SP.J.1006.2014.00571


Transcriptional and Translational Characterization of Rice Chitinase Genes

FAN Wei**,LI Xue-Jiao**,GUAN Ming-Li,MIAO Liu-Yang,SHI Jia-Nan,DOU Shi-Juan,LIU Li-Juan,LI Li-Yun,LIU Guo-Zhen*   

  1. College of Life Sciences, Agricultural University of Hebei, Baoding 071000, China
  • Received:2013-08-08 Revised:2014-01-12 Online:2014-04-12 Published:2014-02-14
  • Contact: 刘国振, E-mail: gzhliu@genomics.org.cn, Tel: 0312-7528250


Plant chitinases play roles in plant development and stress responses. Nineteen chitinase genes, belonging to pathogenesis related (PR) 3 family, were found in rice genome. In this study, constitutive and tissue-specific transcripted chitinase genes were identified, and the domain architecture of chitinase protein was predicted. Clustering analysis and subfamily classification were also carried out. Furthermore, chitinase protein expression profiling was surveyed using western blot (WB), it was found that the expression of CHIT5 was down-regulated in normal growth rice leaves, while the expressions of CHIT6, CHIT14, CHITC1, and CHITC2 were up-regulated. In the incompatible interaction between rice and Xanthomonas oryzae pv. oryzae (Xoo), the expressions of CHIT1, CHIT2, CHIT5, CHIT6, CHIT10, CHIT15, and CHIT16 were enhanced after inoculation and three of CHIT14, CHITC1 and CHITC2 down-regulated. In addition, the comparison of chitinase protein expression in different rice-Xoo interactions showed that these were similar alternation patterns between compatible and incompatible reactions, with a higher extent of alterations in incompatible interactions for most of chitinases. It is interesting to note that the expression of CHIT6 was enhanced in mock-treated samples, suggesting that the expression of CHIT6 may be induced by a mechanic wound. The data revealed in this research will provide useful clues for the understanding of the function of PR3 family chitinase genes.

Key words: Rice, Chitinase, Clustering analysis, Xanthomonas oryzae pv. oryzae (Xoo), Transcription, Western blot

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