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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (05): 798-804.doi: 10.3724/SP.J.1006.2014.00798

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Wheat|Blumeria graminis f. sp. tritici|Resistance gene|Pm2|Molecular marker

SONG Wei1,2,SUN Hui-Gai1,2,SUN Yan-Ling2,ZHAO Zi-Hui1,2,WANG Xiao-Ming2,WU Xiao-Fei2,LI Hong-Jie2,*   

  1. 1 College of Life Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China; 2 National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2013-12-31 Revised:2014-03-04 Online:2014-05-12 Published:2014-03-24

Abstract:

Wennong 14 is a facultative wheat cultivar commercialized in Shandong province and the neighbouring provinces in the northern part of Yellow and Huai River Valleys Facultative Wheat Zone in China. In this study, an array of Blumeria graminis f. sp. tritici (Bgt) isolates were used to test the resistance of Wennong 14 to powdery mildew at both seedling and adult stages. Among the 52 Bgt isolates tested at seedling stage, Wennong 14 was resistant to 43 and susceptible to 9 isolates. The virulence pattern of these Bgt isoaltes on Wennong 14 was similar as that of the known powdery mildew resistance gene Pm2, but the reactions of Wennong 14 to 11 Bgt isolates differed from those of Ulka/8*Cc carrying Pm2. Additionally, Wennong 14 was different from Tabasco carrying Pm46 in the reaction to three isoaltes when tested with 26 Bgt isolates. Wennong 14 was highly resistant to a mixture of Bgt isolates at adult stage. Using segregation populations of F2 and F2:3 developed from the cross of Wennong 14 × Han 4564, genetic analysis demonstrated that the resistance against Bgt isolate E09 was controlled by a single dominant gene, designated PmW14. Based on the results of molecular analysis, PmW14 was linked to markers Xcfd8, Xcfd81, and SCAR203, with genetic distances of 7.5, 1.8, and 7.7 cM, respectively. Because these markers were previously localized on wheat chromosome 5DS in the negion of 5DS-1-0-0.63 and linked to gene Pm2, PmW14 was most likely located on this locus and to be either the same as or an allele of Pm2.

Key words: Wheat, Blumeria graminis f. sp. tritici, Resistance gene, Pm2, Molecular marker

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