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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (09): 1572-1578.doi: 10.3724/SP.J.1006.2014.01572

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning of Zoysiagrass CBF Gene and Validation of Cold Tolerance in Transgenic Arabidopsis

FENG Xun-Wei1,2,CAI Hong-Wei1   

  1. 1 College of Agriculture and Biotechnology, China Agricultural University, Beijing 100190, China; 2 Patent Examination Cooperation Center of the Patent Office, SIPO, Beijing 100190, China
  • Received:2014-03-10 Revised:2014-06-16 Online:2014-09-12 Published:2014-07-09
  • Contact: 才宏伟, E-mail: caihw@cau.edu.cn, Tel: 010-62734224

Abstract:

Zoysiagrass is recognized as an excellent warm-season turfgrass and mainly used in subtropical and tropical regions. Cold stress is a major constraint factor for the cultivation of zoysiagrass. In this study, according to the sequences of cold tolerance gene CBF had been reported in other plant species, we cloned the corresponding homologous of the ZjCBF gene by homology cloning method in Zoysia japonica using a material originated from the most northern area of Japan. Based on the alignment results compared with other reported CBF genes, we found the ZjCBF gene belongs to the CBF1 familiy. By semi-quantitative PCR and Real-time quantitative PCR, we analyzed the expression level of the ZjCBF gene in the cold condition and found that ZjCBF was induced by cold stress, and the ZjCBF expression reached peak after six hours at 4°C treatment. In addition, we also constructed ZjCBF over expression vector and generated transgenic Arabidopsis plants, with better cold tolerance than the wild-type, whether through cold acclimation or not.

Key words: Zoysiagrass, Cold tolerance, CBF transcription factor, Homologous cloning, Transgenic Arabidopsis

[1]Zhu J H, Dong C H, Zhu J K. Interplay between cold-responsive gene regulation, metabolism and RNA processing during plant cold acclimation. Curr Opin Plant Biol, 2007, 10: 290–295



[2]Stocking E J, Gilmour S J, Thomashow M F. Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit. Proc Natl Acad Sci USA, 1997, 94: 1035–1040



[3]Haake V, Cook D, Riechrmnn J L, Pineda O, Thomashow M F, Zhang J Z. Transcription factor CBF4 is a regulator of drought adaptation in Arabidopsis. Plant Physiol, 2002, 130: 639–648



[4]Sakuma Y, Liu Q, Dubouzet J G, Abe H, Shinozaki K, Yamaguchi-Shinozaki K. DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration- and cold- inducible gene expression. Biochem Biophys Res Commun, 2002, 290: 998–1009



[5]Jaglo K R, Kleff S, Amundsen K L, Zhang X, Haake V, Zhang J Z, Deits T, Thmashow M F. Components of the Arabidopsis C-repeat / dehydration-responsive element binding factor cold-response pathway are conserved in Brassica napus and other plant species. Plant Physiol, 2001, 127: 910–917



[6]Hsieh T H, Lee J T, Yang P T, Chiu L H, Chang Y Y, Wang Y C, Chan M T. Heterology expression of the Arabidopsis C-repeat/dehydration response element binding factor 1 gene confers elevated tolerance to chilling and oxidative Stresses in transgenic tomato. Plant Physiol, 2002, 129: 1086–1094



[7]Choi D W, Rodriguez E M, Close T J. Barley cbf3 gene identification, expression pattern, and map location. Plant Physiol, 2002, 129: 1781–1787



[8]Burren M L, Salvi S, Morgante M, Serhani B, Tubelma R. Comparative genomic mapping Between a 745 kb region flanking DREBlA in Arabidopsis thaliana and maize. Plant Mol Biol, 2002, 48: 741–750



[9]Dubouzet J G, Sakuma Y, Ito Y, Kasuga M, Dubonzet E G, Miura S, Seki M, Shinozaki K, Yamaguchi-Shinozaki K. OsDREB genes in rice, Oryza sativa L. encode transcription activators that function in drought-, high-salt and cold-responsive gene expression. Plant J, 2003, 33: 751–763



[10]Rogers O S, Bendich A J. Extraction of DNA from plant tissue. Plant Mol Biol Manual, 1998, A6: 1–10



[11]Yamaguchi-Shinozaki K, Shinozaki K. A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. Plant Cell, 1994, 6: 251–264



[12]Gilmour S J, Zarka D G, Stockinger E J, Salazar M P, Houghton J M, Thomashow M F. Low temperature regulation of the Arabidopsis CBF family of AP2 transcriptional activation as an early step cold-induced COR gene expression. Plant J, 1998, 16: 433–442



[13]Medina J, Bargues M, Terol J, Perez M, Salinas J. The Arabidopsis CBF gene family is composed of three genes encoding AP2 domain-containing proteins whose expression is regulated by low temperature but not by abscisic acid or dehydration. Plant Physiol, 1999, 19: 463–470



[14]Agarwal M, Hao Y, Kapoor A, Dong C H, Hiroaki F, Zheng X, Zhu J K. A R2R3 type MYB transcription factor is involved in the cold regulation of CBF genes and in acquired freezing tolerance. J Biol Chem, 2006, 281: 37636–37645



[15]Liu Q, Kuasga M, Skauma Y, Abe H, Miura S, Yamaguchi-Shinozaki K, Shinozaki K. Two transcription fctors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell, 1998, 10: 1391–1406



[16]Jaglo-Ottoseu K R, Gilmour S J, Zarka D G. Arabidopsis CBF1 overexpression induces COR genes and enhances freezing tolerance. Science, 1998, 280: 104–106



[17]Novillo F, Alonso J M, Ecker J R, Salinas J. CBF2/DREBlC is a negative regulator of CBFl/DREBlB and CBF3/DREBIA expression and plays accentual role in stress tolerance in Arabidopsis. Plant Biol, 2004, 11: 3885–3900

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