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Acta Agron Sin ›› 2018, Vol. 44 ›› Issue (01): 95-104.doi: 10.3724/SP.J.1006.2018.00095


Response of Membrane Fatty Acid Composition and ATPase Activity in Brassica rapa L. to Temperature in North China

FANG Yan1,2,SUN Wan-Cang1,2,*,WU Jun-Yan2,LIU Zi-Gang2,DONG Yun2,3,MI Chao2,MA Li2,CHEN Qi2,HE Hui-Li2   

  1. 1 Gansu Provincial Key Laboratory of Arid Land Crop Sciences, Lanzhou 730070, Gansu, China; 2 Rape Engineering and Technology Research Center of Gansu Provincial, Lanzhou 730070, Gansu, China; 3 Crops Institute of Gansu Academy of Agricultural Sciences, Lanzhou 730070, Gansu, China
  • Received:2017-01-20 Revised:2017-09-10 Online:2018-01-12 Published:2017-09-28
  • Contact: Sun Wancang, E-mail: 18293121851@163.com, Tel: 18293121851
  • Supported by:

    This study was supported by the National Modern Agro-industry Technology System (CARS-13), the National Natural Science Foundation of China (31460356, 31560397), the National Key Basic Research Program of China (973 Program) (2015CB150206), and the Sheng Tongsheng Innovation Fund of Gansu Agricultural University (GSAU-STS-1428).


Brassica rapa L. cultivars Longyou 7 (cold tolerant) and Tianyou 2 (cold sensitive) were used to investigate the variations of membrane fatty acid composition and ATPase activity at the temperatures of 25°C, 10°C, 2°C, and –5°C. There was the same membrane fatty acid composition in leaf and root of two cultivars under temperature stresses, with linolenic acid as the main component of unsaturated fatty acid in leaf, but linoleic acid in root. With decrease of treatment temperatures, the content of unsaturated fatty acid in leaf initially decreased at 10°Cand 2°C, then increased at –5°C.The content of unsaturated fatty acid rose up gradually in root of Longyou 7, but reduced in Tianyou 2. At low temperature(2°C, –5°C), the ratio of U/S and IUFA in Longyou 7 were significantly higher than those in Tianyou 2.The activity of ATPase was gradually increased in Longyou 7 than in Tianyou 2. It suggested there are differences in membrane lipids in response to temperature for two winter rapeseed cultivars, the increase of unsaturated fatty acid content and ATPase activity is the main cause sustaining winter rapeseed cultivars to overwinter.

Key words: winter rapeseed, fatty acid, ATPase activity, cold tolerance

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