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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (10): 1711-1716.doi: 10.3724/SP.J.1006.2014.01711

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS •     Next Articles

Genetic Analysis and Gene Fine Mapping of Rice Leafy Head Mutant s2-21

WANG Hai-Feng1,GAO Jie1,SUN Wei1,ZHANG Shi-Yong1,ZHAO Qing-Lei1,YIN Liang1,ZHAO Jin-Feng2,LI Xue-Yong2,*,YUAN Shou-Jiang1,*   

  1. 1 Shandong Rice Research Institute, Jinan 250100, China; 2 National Key Facility for Crop Gene Resource and Geneic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2014-03-14 Revised:2014-07-06 Online:2014-10-12 Published:2014-07-25
  • Contact: 李学勇, E-mail: lixueyong@caas.cn, Tel: 010-82107409; 袁守江, E-mail: ysj868@sina.com, Tel: 15615973691 E-mail:13256706321@163.com

Abstract:

Phyllotaxy and plastochron are basic aspects of leaf development and the important agronomic traits of rice .The study of phyllotaxy or plastochron mutant will help us to understand the mechanism of leaf formation. In this study, a leafy head mutant s2-21 was isolated from an EMS (ethyl methane sulfonate) mutagenized japonica cultivar Nipponbare. It was characterized by shorter intervals between leaves, shortened internodes, dwarf plants, decreased tiller number, and increased leaf number. It was also unable to transit from vegetative to reproductive growth. Genetic analysis with a F2 population of the mutant and an indica cultivar Dular showed that this trait is controlled by a single recessive nucleus gene, which was primarily mapped between two insertion-deletion (InDel) markers C1-15 and S1-17 on chromosome 1. For the fine mapping, new InDel markers were designed by utilizing information of genomic sequences from Nipponbare (http://www.ncbi.nlm.nih.gov/) and Dular (our unpublished data), and eventually the gene was localized within the region of 88 kb between W25 and W26. Sequence analysis indicated the mutant was caused by mutation of the fifth base from G to A in the fourth intron of the pla2 gene.

Key words: Rice, Leafy head mutant, Plastochron, Gene mapping, PLA2

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