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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (10): 1748-1755.doi: 10.3724/SP.J.1006.2014.01748

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Expression Analysis of LPAT4 Gene from Brassica napus

XIAO Dan-Wang1,LIU Cong1,HU Xue-Fang1,CHEN She-Yuan2,GUAN Chun-Yun2,XIONG Xing-Hua1,2,*   

  1. 1 Crop Gene Engineering Key Laboratory of Hunan Province, Hunan Agricultural University, Changsha 410128, China; 2 Hunan Branch of National Oilseed Crops Improvement Centre, Hunan Agricultural University, Changsha 410128, China
  • Received:2014-03-10 Revised:2014-07-06 Online:2014-10-12 Published:2014-07-25
  • Contact: 熊兴华, E-mail: ndxiongene@yahoo.com, Tel: 13508487613

Abstract:

Lysophospholipid acid actyltransferase (LPAT) is a pivotal enzyme of triacylglycerol biosynthesis, which plays a key role in lipid synthesis, development of plant seeds and bio-membrane fluidity. Through the technology of homology-based cloning, two copies of LPAT4 full-length CDS sequences (1143 bp and 1140 bp) were cloned in this study, designated as BnLPAT4-1and BnLPAT4-2, respectively. Bioinformatics analysis revealed that they shared the LPLAT_LCLAT1 like domain and belonged to the LPLAT superfamily. Temporal and spatial expression results showed that BnLPAT4-1 and BnLPAT4-2 were constitutive expression genes. Among them, the highest expression of BnLPAT4-1 was in leaf, while that ofBnLPAT4-2 was in embryo. Stresses analysis indicated that BnLPAT4-1and BnLPAT4-2 presented different expression patterns under the treatments of NaCl, PEG4000, waterlogging, 6BA and ABA. Pole difference analysis displayed that ABA had a great effect on the expression of BnLPAT4-1, while BnLPAT4-2 was more sensitive to PEG-4000.The results provided a base for the research of regulation and function of BnLPAT4 in Brassica napus.

Key words: LPAT4, Brassica Napus, Temporal and spatial expression, Abiotic stress, Expression analysis

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