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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (10): 1557-1563.doi: 10.3724/SP.J.1006.2015.01564

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effect of Brassinolide on Leaf Photosynthetic Function and Yield in Spring Maize Filling Stage

WANG Qing-Yan1, GUAN Da-Hai1,2,PAN Hai-Bo1,LI Jian-Min1,DUAN Liu-Sheng1,ZHANG Ming-Cai1,*,LI Zhao-Hu1   

  1. 1 Engineering Research Center of Plant Growth Regulator, Ministry of Education / College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China; 2 Rural Energy and Environment Agency, Ministry of Agriculture, Beijing 100125, China
  • Received:2015-02-15 Revised:2015-06-01 Online:2015-10-12 Published:2015-06-05
  • Contact: 张明才, E-mail: zmc1214@163.com, Tel: 010-62733049 E-mail:wangqyan@aliyun.com

Abstract:

A field experiment was conducted to investigate the effect of plant growth regulator brassinolide (BR) on the regulation of physiological activities of ear leaf, the grain filling and yield in spring maize (Zea mays L., cv. Zhengdan 958). Husks and ear leaves were treated with BR at 15 days after anthesis by foliar spraying. The results showed that the chlorophyll content, photosynthetic rate and activities of PEP carboxylaes, RuBP carboxylase, sucrose synthase and sucrose phosphate synthase in ear leaf as well as source content in kernel were significantly reduced, while starch accumulation and dry weight of kernel were increased during grain filling. BR significantly increased the content of chlorophyll and photosynthetic rate, enhanced the activities of PEP carboxylaes and RuBP carboxylase, definitely resulting in enhanced CO2 assimilation. BR markedly increased the activities of sucrose synthase and sucrose phosphate synthase in ear leaf. And in kernel, the accumulation of sucrose and starch as well as the grain filling rate were accelerated by BR. BR treatment significantly reduced the length of ear barren tip, and increased the number of kernels per ear, which led to higher yield. These results suggest that BR treatment at filling stage may be a good strategy to get a high yield in maize, by enhancing activity of resource, delaying sencence and stimulating the ?ow of assimilate from the source to the sink.

Key words: Brassinolide, Maize, Grain filling, Photosynthetic function, Yield

[1]Yang H, Zehnder A. China’s regional water scarcity and implications for grain supply and trade. Environ Plan A, 2001, 33: 79–95



[2]陈显奇. 玉米早衰原因的探讨. 杂粮作物, 2002, 22(1): 27–28



Chen X Q. Study of the causes of maize premature senescence. Rain Fed Crops, 2002, 22(1): 27–28 (in Chinese)



[3]Reid J B. Phytohormone mutants in plant research. J Plant Growth Regul,1990, 9: 97–111



[4]Tanner W. On the possible role of ABA on phloem unloading. Ber Dtsch Bot Ges, 1980, 93: 349–351



[5]Gudesblat G E, Schneider-Pizo J, Betti C, Mayerhofer J, Vanhoutte I, van Dongen W, Boeren S, Zhiponova M, de Vries S, Jonak C. SPEECHLESS integrates brassinosteroid and stomata signalling pathways. Nat Cell Biol, 2012, 14: 548–554



[6]Wang Z Y, Bai M Y, Oh E, Zhu J Y. Brassinosteroid signaling network and regulation of photomorphogenesis. Annu Rev Genet, 2012, 46: 701–724



[7]Ye Q, Zhu W, Li L, Zhang S, Yin Y, Ma H, Wang X. Brassinosteroids control male fertility by regulating the expression of key genes involved in Arabidopsis anther and pollen development. Proc Natl Acad Sci USA, 2010, 107: 6100–6105



[8]Hartwig T, Chuck G S, Fujioka S, Klempien A, Weizbauer R, Potluri D P V, Choee S, Johal G S, Schulz B. Brassinosteroid control of sex determination in maize. Proc Natl Acad Sci USA, 2011, 108: 19814–19819



[9]Jiang W B, Huang H Y, Hu Y W, Zhu S W, Wang Z Y, Lin W H. Brassinosteroid regulates seed size and shape in Arabidopsis. Plant Physiol, 2013, 162: 1965–1977



[10]Morinaka Y, Sakamoto T, Inukai Y, Agetsuma M, Kitano H, Ashikari M, Matsuoka M. Morphological alteration caused by brassinosteroid insensitivity increases the biomass and grain production of rice. Plant Physiol, 2006, 141: 924–931



[11]李立人, 王维光, 韩祺. 苜蓿二磷酸核酮糖(RuBP)羧化酶体内活化作用的调节. 植物生理与分子生物学学报, 1986, 1: 35–41



Li L R, Wang W G, Han Q. The regulation of ribulose-1,5-biosphospate carboxylase activation in alfalfa leaves. J Plant Physiol Mol Biol, 1986, 1: 35–41 (in Chinese)



[12]Racker E. Ribulose diphosphate carboxylase from spinach leaves. In: Colowick S P, Kaplan N O, eds. Methods in Enzymology. New York: Academic Press, l962. pp 266–278



[13]施教耐, 吴敏贤, 查静娟. 植物磷酸烯醇式丙酮酸羧化酶的研究: I. PEP羧化酶同功酶的分离和变构特性的比较. 植物生理与分子生物学学报, 1979, 5: 225–236



Shi J N, Wu M X, Zha J J. Studies on plant phosphoenolpyruvate carboxylase: I. Separation and properties of PEP carboxylase isoenzymes. J Plant Physiol Mol Biol, 1979, 5: 225–236 (in Chinese) 



[14]Douglas C, Doehelter T, Tsung M K, Frederick C F. Enzymes of sucrose and hexose metabolism in developing hernels of two inbreds of maize. Plant Physiol, 1988, 86: 1013–1019



[15]苏丽英, 吴勇, 於新建, 夏叔芳. 水稻叶片蔗糖磷酸合成酶的一些特性. 植物生理学报, 1989, 15: 117–123



Su L Y, Wu Y, Yu J X, Xia S F. Some properties of rice leaf sucrose phosphate synthetase. Acta Physiol Sin, 1989, 15: 117–123 (in Chinese with English abstract)



[16]Wardlaw I F. The early stages of grain development in wheat: response to water stress in a single variety. Aust J Biol Sci, 1994, 44: 1047–1055



[17]何照范. 粮油籽粒品质及其分析技术. 北京: 农业出版社, 1985. pp 290–294



He Z F. Analysis Technique for Grain Quality of Cereals and Oils. Beijing: Agriculture Press, 1985. pp 290–294 (in Chinese)



[18]张志良, 瞿伟菁. 植物生理学实验指导. 北京: 高等教育出版社, 2004. pp 128–129



Zhang Z L, Qu W J. Guidance of Plant Physiology Experiment. Beijing: Higher Education Press, 2004. pp 128–129 (in Chinese)



[19]Emes M J, Bowsher C G, Hedley C, Burrell M M, Scrase-Field E S F, Tetlow I J. Starch synthesis and carbon partitioning in developing endosperm. J Exp Bot, 2003, 54: 569–575



[20]肖琳, 庞瑞华, 蔡荣先, 于萍, 黄新华, 王磊. 水稻初花期喷施油菜素内酯的生理效应及增产作用. 安徽农业科学, 2007, 35(11): 3317–3330



Xiao L, Pang R H, Cai R X, Yu P, Huang X H, Wang L. Physiological effect and yield increase action after spraying BR in rice early blooming stage. J Anhui Agric Sci, 2007, 35: 3317–3330 (in Chinese with English abstract)



[21]Wu C Y, Trieu A, Radhakrishnan P, Kwok S F, Harris S, Zhang K, Wang J, Wan J, Zhai H, Takatsuto S, Matsumoto S, Fujioka S, Feldmann K A, Roger I P. Brassinosteroids regulate grain ?lling in rice. Plant Cell, 2008, 20: 2130–2145



[22]王士银, 陈冉冉, 石庆华, 张桦, 赵明, 徐江. 开花期外施表油菜素内酯(epi-BR)对水稻的影响. 作物杂志, 2012, (4): 83–85



Wang S Y, Chen R R, Shi Q H, Zhang H, Zhao M, Xu J. The effects of epi-BR treated in primary flowering stage on rice. Crops, 2012, (4): 83–85 (in Chinese with English abstract)



[23]Hong Z, Ueguchi-Tanaka M, Fujioka F, Takatsuto S, Yoshida S, Hasegawa Y, Ashikari M, Kitano H, Matsuoka M. The rice brassinosteroid-deficient dwarf2 mutant, defective in the rice homolog of Arabidopsis DIMINUTO/DWARF1, is rescued by the endogenously accumulated alternative bioactive brassinosteroide, dolichosterone. Plant Cell, 2005, 17: 2243–2254



[24]Morinaka Y, Sakamoto T, Inukai Y, Agetsuma M, Kitano H, Ashikari M, Matsuoka M. Morphological alteration caused by brassinosteroid insensitivity increases the biomass and grain production of rice. Plant Physiol, 2006, 141: 924–931



[25]刘海英, 郭天财, 朱云集, 王晨阳, 康国章. 开花期外施表油菜素内酯(epi-BR)对小麦籽粒淀粉积累及其关键酶活性的影响. 作物学报, 2006, 32: 924–930



Liu H Y, Guo T C, Zhu Y J, Wang C Y, Kang G Z. Effects of epi-brassinolide (epi-BR) application at anthesis on starch accumulation and activities of key enzymes in wheat grains. Acta Agron Sin, 2006, 32: 924–930 (in Chinese with English abstract)



[26]Huang H Y, Jiang W B, Hu Y W, Wu P, Zhu J Y, Liang W Q, Wang Z Y, Lin W H. BR signal in?uences Arabidopsis ovule and seed number through regulating related genes expression by BZR1. Mol Plant, 2012, 6: 456–469



[27]Zhou Y, Zhang X, Kang X, Zhao X, Zhang X, Ni M. SHORT HYPO-COTYL UNDER BLUE1 associates with MINISEED3 and HAIKU2 promoters in vivo to regulate Arabidopsis seed development. Plant Cell, 2009, 21: 106–117

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