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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (05): 667-674.doi: 10.3724/SP.J.1006.2016.00667


Physiological Characteristics and Gene Mapping of a Precocious Leaf Senescence Mutant ospls3 in Rice

GONG Pan1,**,LI Kun-Yu1,**,HUANG Fu-Deng2,WEI Li-Quan1,YANG Xi1,CHENG Fang-Min1,PAN Gang1,*   

  1. 1 College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China; 2 Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
  • Received:2015-09-12 Revised:2016-01-11 Online:2016-05-12 Published:2016-01-19
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31271691) and the Major Project of China on New Varieties of GMO Cultivation (2013ZX08001-002).


Leaf senescence is the final stage of leaf development. However, premature aging of functional leaves leads to yield reduction and quality decline. Thus, it is very important for developing novel crop germplasms with delayed leaf-senescence characteristics through investigating the molecular mechanism of leaf senescence. In this study, an ospls3 (Oryza sativa precocious leaf senescence 3) mutant, produced by 60Co γ-radiation treatment of indica cultivar N142, was identified. The symptoms of the premature senescence mutant presented firstly at tillering stage showing brown leaf tip and brown spots in top part of leaf blade, then spread rapidly to basal part of leaf blade and led leaf to die. The physiological analysis indicated that, in the ospls3 mutant, the content of chlorophyll was the highest in the flag leaf, the following was in second-top and third-top leaves, but all of them were significantly lower than those in the wild type. The contents of MDA, O2?, and H2O2 and the activities of SOD and POD among these top three leaves in the wild type maintained similar levels, which were significantly lower than those in the mutant type. The soluble protein contents and the activity of CAT had no significant difference among top three leaves in the wild type while significantly decreased in the mutant. Genetic analysis verified that the ospls3 is a recessive mutant and was mapped in a 294 kb interval between RM6953 and RM28753 on the long arm of chromosome 12, which establishes a solid foundation for further cloning and functional studies of this gene.

Key words: Rice, ospls3, Precocious leaf senescence, Physiological analysis, Gene mapping

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