Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (04): 522-529.doi: 10.3724/SP.J.1006.2017.00522

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Analysis and Gene Mapping of Yellow leaf and dwarf (yld) mutant in Rice

LI Zi-Zhuang1,2,**,XU Qian-Kun2,**,YU Hai-Ping2,ZHOU Ting-Ting2,XUE Da-Wei1,ZENG Da-Li2,GUO Long-Biao2,QIAN Qian2,*,REN De-Yong2,*   

  1. 1 College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310006, China; 2 State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China
  • Received:2016-07-19 Revised:2016-11-02 Online:2017-04-12 Published:2016-11-11
  • Contact: 任德勇, E-mail: rendeyong616@163.com; 钱前, E-mail: qianqian188@hotmail.com E-mail:15906682710@163.com
  • Supported by:

    The study was supported by the National Natural Science Foundation of China (31401464).

Abstract:

Leaf color mutants of rice are ideal materials in studies on photosynthesis, chlorophyll metabolism and chloroplast development in plants. A yellow leaf and dwarf mutant yldwas obtained from ethyl methane sulfonate (EMS)-treated Shuhui 527 (Oryza sativa L.). Compared with the wild type, the yld mutant showed yellow leaf and dwarfism, and the contents of chlorophyll and carotenoid were obviously decreased. Transmission electron microscope observation revealed that the structure of most chloroplasts seemed to be normal, however, with the fuzzy grana, and fewer and looser stroma lamella in the yld mutant. Meantime, plant height, branch number, grain number per panicle, 1000-grain weight and seed-setting rate were significantly decreased, while the number of effective panicle was obviously increased in the ylda single recessive gene. The 323 mutational individuals from the F2 generation of the cross of Wuyunjing 7 and yld mutant were used for gene mapping. Finally, the YLD locus was mapped on chromosome 11 between two Indel markers L5 and L7, with an approximate 115 kb physical region. This result would facilitate cloning and functional analysis for the YLD gene. mutant compared with those in the wild type. Genetic analysis showed that the yld mutant traits were controlled by

Key words: Rice, Yellow leaf and dwarf mutant, Genetic analysis, Gene mapping

[1] Morita R, Sato Y, Masuda Y, Nishimura M, Kusaba M. Defect innon-yellow coloring 3, an a/b hydrolase-fold family protein, causes a stay-green phenotype during leaf senescence in rice. Plant J, 2009, 59: 940–952
[2] Wu Z, Zhang X, He B, Diao L, Sheng S, Wang J, Guo X, Su N, Wang L, Jiang L, Wang C, Zhai H, Wan J. A chlorophyll-deficient rice mutant with impaired chlorophyllide esterification in chlorophyll biosynthesis. Plant Physiol, 2007, 145: 29–40
[3] Larkin R M, Alonso J M, Ecker J R, Chory J. GUN4, a regulator of chlorophyll synthesis and intracellular signaling. Science, 2003, 299: 902–906
[4] Sugimoto H, Kusumi K, Tozawa Y, Yazaki J, Kishimoto N, Kikuchi S, Iba K. The virescent-2mutation inhibits translation of plastid transcripts for the plastidgenetic system at an early stage of chloroplast differentiation. Plant Cell Physiol, 2004, 45: 985–996
[5] Kusumi K, Sakata C, Nakamura T, Kawasaki S, Yoshimura A, Iba K. A plastid protein NUS1 is essential for build-up of the genetic system for early chloroplast development under cold stress conditions. Plant J, 2011, 68: 1039–1050
[6] Goh C H, Satoh K, Kikuchi S, Kim S C, Ko S M, Kang H G, Jeon J S, Kim C S, Park Y I. Mitochondrial activity in illuminated leaves of chlorophyll-deficient mutant rice (OsCHLH) seedlings. Plant Biotechnol Rep, 2010, 4: 281–291
[7] Awan M A, Konzak C F, Rutger J N, Nilan R A. Mutagenic effects of sodium azide in rice. Crop Sci, 1979, 20: 663–668
[8] Lee S, Kim J H, Yoo E S, Lee C H, Hirochika H, An G. Differential regulation of chlorophyll a oxygenase genes in rice. Plant Mol Biol, 2005, 57: 805–818
[9] 刘文真. 三个水稻叶色突变体的鉴定与基因克隆. 浙江大学博士学位论文, 浙江杭州, 2006
   Liu W Z. Characterization of Three Chlorophyll Deficient Mutants in Rice. PhD Dissertation of Zhejiang University, Hangzhou, China, 2006 (in Chinese with English abstract)
[10] Wu Z M, Zhang X, He B, Diao L P, Sheng S L, Wang J L, Guo X P, Su N, Wang L F, Jiang L, Wang C M, Zhai H Q, Wan J M. A chlorophyll-deficient rice mutant with impaired chlorophyllide esterification in chlorophyll biosynthesis. Plant Physiol, 2007, 145: 29–40
[11] Jung K H, Hur J, Ryu C H, Choi Y, Chung Y Y, Miyao A, Hirochika H, An G. Characterization of a rice chlorophyll-deficient mutant using the T-DNA gene-trap system. Plant Cell Physiol, 2003, 44: 463–472
[12] Zhang H T, Li J J, Yoo J H, Yoo S C, Cho S H, Koh H J, Seo H S, Paek N C. Rice Chlorina-1 and Chlorina-9 encode Chl D and Chl I subunits of Mg-chelatase, a key enzyme for chlorophyll synthesis and chloroplast development. Plant Mol Biol, 2006, 62: 325–337
[13] Kusaba M, Ito H, Morita R, Iida S, Sato Y, Fujimoto M, Kawasaki S, Tanaka R, Hirochika H, Nishimura M, Tanaka A. Rice Non-yellow Coloring 1 is involved in light-harvesting complex II and grana degradation during leaf senescence. Plant Cell, 2007, 19: 1362–1375
[14] Sato Y, Morita R, Katsuma S, Nishimura M, Tanaka A, Kusaba M. Two short-chain dehydrogenase/reductases, Non-yellow Coloring 1 and Nyc1-like, are required for chlorophyll b and light-harvesting complex Ⅱ degradation during senescence in rice. Plant J, 2009, 57: 120–131
[15] Zhang Z M, Tan J J, Shi Z Y, Xie Q J, Xing Y, Liu C H, Chen Q L, Zhu H T, Wang J, Zhang J L, Zhang G Q. Albino leaf 1 that encodes the sole octotricopeptide repeat protein is responsible for chloroplast development in rice. Plant Physiol, 2016, 171: 1182–1191
[16] Li C M, Hu Y, Huang R, Ma X Z, Wang Y, Liao T T, Zhong P, Xiao F L, Sun C H, Xu Z J, Deng X J, Wang P R. Mutation of FdC2 gene encoding a ferredoxin-like protein with C-terminal extension causes yellow-green leaf phenotype in rice. Plant Sci, 2015, 238: 127–134
[17] Yang Y L, Xu J, Huang L C, Leng Y J, Dai L P, Rao Y C, Chen L, Wang Y Q, Tu Z J, Hu J, Ren D Y, Zhang G H, Zhu L, Guo L B, Qian Q, Zeng D L. PGL, encoding chlorophyllide a oxygenase 1, impacts leaf senescence and indirectly affects grain yield and quality in rice. J Exp Bot, 2015, 67: 1297–1310
[18] Sakuraba Y, Rahman M L, Cho S H, Kim Y S, Koh H J, Yoo S C, Paek N C. The rice faded green leaf locus encodes protochlorophyllide oxidoreductase B and is essential for chlorophyll synthesis under high light conditions. Plant J, 2013, 74: 122–133
[19] Fang J, Chai C L, Qian Q, Li C L, Tang J Y, Sun L, Huang Z J, Guo X L, Sun C H, Liu M, Zhang Y, Lu Q T, Wang Y Q, Lu C M, Han B, Chen F, Cheng Z K, Chu C C. Mutations of genes in synthesis of the carotenoid precursors of ABA lead to pre-harvest sprouting and photo-oxidation in rice. Plant J, 2008, 54: 177–189
[20] Lichtenthaler H K. Chlorophylls and carotenoids: Pigments of photosynthetic biomenbranes. Meth Enzymol, 1987, 148: 350–382
[21] 何瑞峰, 丁毅, 余金洪, 祖明生. 水稻温敏叶绿素突变体叶片超微结构的研究. 武汉植物学研究, 2001, 19: 1–5
He R F, Ding Y, Yu J H, Zu M S. Study on leaf ultrastructure of the thermo-sensitive chlorophyll deficient mutant in rice. J Wuhan Bot Res, 2001, 19: 1–5 (in Chinese with English abstract)
[22] Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA, 1991, 88: 9828–9832
[23] Rogers S O, Bendich A J. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol, 1985, 5: 69–76
[24] Kosambi D D. The estimation of map distances from recombination values. Ann Hum Genet, 1944, 12: 172–175
[25] 王亚琴, 施军琼, 张婷, 李燕, 张天泉, 张小龙, 桑贤春, 凌英华, 何光华. 水稻绿叶突变体ygl13的鉴定及候选基因分析. 中国农业科学, 2015, 48: 4197–4208
Wang Y Q, Shi J Q, Zhang T, Li Y, Zhang T Q, Zhang X L, Sang X C, Ling Y H, He G H. Characterization and candidate gene analysis of yellow-green leaf mutant ygl13 in rice. Sci Agric Sin, 2015, 48: 4197–4208 (in Chinese with English abstract)
[26] Zhang F T, Luo X D, Hu B L, Wan Y, Xie J K. YGL138(t), encoding a putative signal recognition particle 54 kDa protein, is involved in chloroplast development of rice. Rice, 2013, 6: 7
[27] Mao D H, Yu H H, Liu T M, Yang G Y, Xing Y Z. Two complementary recessive genes in duplicated segments control etiolation in rice. Theor Appl Genet, 2011, 122: 373–383
[28] Han S H, Sakuraba Y, Koh H J, Paek N C. Leaf variegation in the rice zebra2 mutant is caused by photoperiodic accumulation of tetra-cis-lycopene and singlet oxygen. Mol Cells, 2012, 33: 87–97
[29] Xing C, Wang G X, Huang J L, Wu J Z. Research on chlorophyll mutation of plants and molecular mechanism. Biotechnol Bull, 2008, 5: 10–12

[1] TIAN Tian, CHEN Li-Juan, HE Hua-Qin. Identification of rice blast resistance candidate genes based on integrating Meta-QTL and RNA-seq analysis [J]. Acta Agronomica Sinica, 2022, 48(6): 1372-1388.
[2] ZHENG Chong-Ke, ZHOU Guan-Hua, NIU Shu-Lin, HE Ya-Nan, SUN wei, XIE Xian-Zhi. Phenotypic characterization and gene mapping of an early senescence leaf H5(esl-H5) mutant in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2022, 48(6): 1389-1400.
[3] ZHOU Wen-Qi, QIANG Xiao-Xia, WANG Sen, JIANG Jing-Wen, WEI Wan-Rong. Mechanism of drought and salt tolerance of OsLPL2/PIR gene in rice [J]. Acta Agronomica Sinica, 2022, 48(6): 1401-1415.
[4] ZHENG Xiao-Long, ZHOU Jing-Qing, BAI Yang, SHAO Ya-Fang, ZHANG Lin-Ping, HU Pei-Song, WEI Xiang-Jin. Difference and molecular mechanism of soluble sugar metabolism and quality of different rice panicle in japonica rice [J]. Acta Agronomica Sinica, 2022, 48(6): 1425-1436.
[5] YAN Jia-Qian, GU Yi-Biao, XUE Zhang-Yi, ZHOU Tian-Yang, GE Qian-Qian, ZHANG Hao, LIU Li-Jun, WANG Zhi-Qin, GU Jun-Fei, YANG Jian-Chang, ZHOU Zhen-Ling, XU Da-Yong. Different responses of rice cultivars to salt stress and the underlying mechanisms [J]. Acta Agronomica Sinica, 2022, 48(6): 1463-1475.
[6] YANG Jian-Chang, LI Chao-Qing, JIANG Yi. Contents and compositions of amino acids in rice grains and their regulation: a review [J]. Acta Agronomica Sinica, 2022, 48(5): 1037-1050.
[7] DENG Zhao, JIANG Nan, FU Chen-Jian, YAN Tian-Zhe, FU Xing-Xue, HU Xiao-Chun, QIN Peng, LIU Shan-Shan, WANG Kai, YANG Yuan-Zhu. Analysis of blast resistance genes in Longliangyou and Jingliangyou hybrid rice varieties [J]. Acta Agronomica Sinica, 2022, 48(5): 1071-1080.
[8] YANG De-Wei, WANG Xun, ZHENG Xing-Xing, XIANG Xin-Quan, CUI Hai-Tao, LI Sheng-Ping, TANG Ding-Zhong. Functional studies of rice blast resistance related gene OsSAMS1 [J]. Acta Agronomica Sinica, 2022, 48(5): 1119-1128.
[9] ZHU Zheng, WANG Tian-Xing-Zi, CHEN Yue, LIU Yu-Qing, YAN Gao-Wei, XU Shan, MA Jin-Jiao, DOU Shi-Juan, LI Li-Yun, LIU Guo-Zhen. Rice transcription factor WRKY68 plays a positive role in Xa21-mediated resistance to Xanthomonas oryzae pv. oryzae [J]. Acta Agronomica Sinica, 2022, 48(5): 1129-1140.
[10] WANG Xiao-Lei, LI Wei-Xing, OU-YANG Lin-Juan, XU Jie, CHEN Xiao-Rong, BIAN Jian-Min, HU Li-Fang, PENG Xiao-Song, HE Xiao-Peng, FU Jun-Ru, ZHOU Da-Hu, HE Hao-Hua, SUN Xiao-Tang, ZHU Chang-Lan. QTL mapping for plant architecture in rice based on chromosome segment substitution lines [J]. Acta Agronomica Sinica, 2022, 48(5): 1141-1151.
[11] WANG Ze, ZHOU Qin-Yang, LIU Cong, MU Yue, GUO Wei, DING Yan-Feng, NINOMIYA Seishi. Estimation and evaluation of paddy rice canopy characteristics based on images from UAV and ground camera [J]. Acta Agronomica Sinica, 2022, 48(5): 1248-1261.
[12] KE Jian, CHEN Ting-Ting, WU Zhou, ZHU Tie-Zhong, SUN Jie, HE Hai-Bing, YOU Cui-Cui, ZHU De-Quan, WU Li-Quan. Suitable varieties and high-yielding population characteristics of late season rice in the northern margin area of double-cropping rice along the Yangtze River [J]. Acta Agronomica Sinica, 2022, 48(4): 1005-1016.
[13] CHEN Yue, SUN Ming-Zhe, JIA Bo-Wei, LENG Yue, SUN Xiao-Li. Research progress regarding the function and mechanism of rice AP2/ERF transcription factor in stress response [J]. Acta Agronomica Sinica, 2022, 48(4): 781-790.
[14] WANG Hao-Rang, ZHANG Yong, YU Chun-Miao, DONG Quan-Zhong, LI Wei-Wei, HU Kai-Feng, ZHANG Ming-Ming, XUE Hong, YANG Meng-Ping, SONG Ji-Ling, WANG Lei, YANG Xing-Yong, QIU Li-Juan. Fine mapping of yellow-green leaf gene (ygl2) in soybean (Glycine max L.) [J]. Acta Agronomica Sinica, 2022, 48(4): 791-800.
[15] LIU Lei, ZHAN Wei-Min, DING Wu-Si, LIU Tong, CUI Lian-Hua, JIANG Liang-Liang, ZHANG Yan-Pei, YANG Jian-Ping. Genetic analysis and molecular characterization of dwarf mutant gad39 in maize [J]. Acta Agronomica Sinica, 2022, 48(4): 886-895.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!