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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (04): 522-529.doi: 10.3724/SP.J.1006.2017.00522


Genetic Analysis and Gene Mapping of Yellow leaf and dwarf (yld) mutant in Rice

LI Zi-Zhuang1,2,**,XU Qian-Kun2,**,YU Hai-Ping2,ZHOU Ting-Ting2,XUE Da-Wei1,ZENG Da-Li2,GUO Long-Biao2,QIAN Qian2,*,REN De-Yong2,*   

  1. 1 College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310006, China; 2 State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China
  • Received:2016-07-19 Revised:2016-11-02 Online:2017-04-12 Published:2016-11-11
  • Contact: 任德勇, E-mail: rendeyong616@163.com; 钱前, E-mail: qianqian188@hotmail.com E-mail:15906682710@163.com
  • Supported by:

    The study was supported by the National Natural Science Foundation of China (31401464).


Leaf color mutants of rice are ideal materials in studies on photosynthesis, chlorophyll metabolism and chloroplast development in plants. A yellow leaf and dwarf mutant yldwas obtained from ethyl methane sulfonate (EMS)-treated Shuhui 527 (Oryza sativa L.). Compared with the wild type, the yld mutant showed yellow leaf and dwarfism, and the contents of chlorophyll and carotenoid were obviously decreased. Transmission electron microscope observation revealed that the structure of most chloroplasts seemed to be normal, however, with the fuzzy grana, and fewer and looser stroma lamella in the yld mutant. Meantime, plant height, branch number, grain number per panicle, 1000-grain weight and seed-setting rate were significantly decreased, while the number of effective panicle was obviously increased in the ylda single recessive gene. The 323 mutational individuals from the F2 generation of the cross of Wuyunjing 7 and yld mutant were used for gene mapping. Finally, the YLD locus was mapped on chromosome 11 between two Indel markers L5 and L7, with an approximate 115 kb physical region. This result would facilitate cloning and functional analysis for the YLD gene. mutant compared with those in the wild type. Genetic analysis showed that the yld mutant traits were controlled by

Key words: Rice, Yellow leaf and dwarf mutant, Genetic analysis, Gene mapping

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