Supplementary table 1

Origins of 229 wheat cultivars (lines) and their His and PFT genotypes"

Supplementary table 2

Detection of Fhb1 in 73 Yangmai and Ningmai cultivars (lines)"

Table 1

Specific primers used to amplify the open reading frames (ORF) of PFT, His, and HC"

Table 2

Analysis of variance (ANOVA) of Fusarium head blight index in 229 wheat cultivars"

Fig. 1

Structure, primer binding sites and three alleles of PFT gene The first nucleotide of the initiation codon reads as +1"

Fig. 2

Amplification profiles of marker PFT-CAPS in partial Chinese cultivars M: DL5000 DNA marker; 1-4: PFT-I cultivars, namely Sumai 3, Ningmai 9, Xinong 9871, and Xiaoyan 22; 5-8: PFT-II cultivars, namely Zhengmai 366, Lumai 21, Hengguan 35, and Ningchun 4. PCR products were digested with Dra I restriction endonuclease."

Table 3

Alleles of His and HC genes for the cultivars with PFT-I and their FHB indexes"

Fig. 3

Structure, primer binding sites, and alleles of HC and His genes for the cultivars with PFT-I"

Fig. 4

Amplification profiles of marker His-InDel in partial Chinese cultivars M: DL5000 DNA marker; 1-4: His-I cultivars, namely Sumai 3, Ning 7840, Ningmai 9, and Ningmai 13; 5-11: non His-I cultivars, namely Xinong 9871, Xiaoyan 22, Zhengmai 9023, Yan 2415, Yangmai 158, Jimai 22, and Aikang 58."

Fig. 5

Average FHB indexes of cultivars or lines with different PFT/His haplotypes"

Fig. 6

Gene flow of Fhb1 in 73 cultivars (lines) of Yangmai and Ningmai through founder parent Ningmai 9 Cultivars or lines in solid boxes were characterized carrying Fhb1 using PFT-CAPS and His-InDel markers, while those in dotted boxes were uncharacterized."

Supplementary table 3

Supplementary table 3 Pedigrees of Chinese wheat cultivars (lines) carrying Fhb1"