玉米苗期根汞胁迫响应中miRNA的鉴定及初步验证

doi:10.3724/SP.J.1006.2022.13074

Abstract

Abstract:

Mercury is one of the most important sources of heavy mental pollution to crop production in the worldwide. MicroRNA (miRNA) is a critical regulator in plant development and abiotic stress responses. However, its function on mercury stress response is still unknown in monocots. To identify the critical miRNA in response to mercury, we analyzed phenotype changes and differentially expressed miRNA in seedlings of two maize inbred lines B73 and Zheng 58 (Z58) under HgCl₂ stress. The results showed that B73 was more sensitive to mercury than Z58 and miRNA166l was down-regulated in both B73 and Z58 under HgCl₂ stress. miRNA165/166 knock-out stable lines of Arabidopsis were created using STTM technology to validate its role in mercury stress response. These lines showed wilted and etiolated leaf and shorten root, which were similar with maize seedlings after mercury treatment. This study verified that miRNA166 was important for mercury stress modulation, which promoted us to explore the molecular mechanism of miRNA166 in heavy metal response in the following experiments.

Key words: maize, mercury stress, microRNA, STTM

QIN Yong-Tian, CHEN Li-Xia, TANG Ji-Hua, CHEN Jian-Hui, MA Shuan-Hong, ZHANG Xue-Hai, DING Dong, FU Zhi-Yuan. Identification and validation of miRNA involved in mercury stress response in maize seedling roots[J].Acta Agronomica Sinica, 2022, 48(12): 3018-3028.

Figures/Tables 6

Fig. 1

Fig. 2

Table 1

Table 2

Differentially expressed miRNA in B73 and Z58 after HgCl2 stress"

B73			郑58 Zheng 58
基因序列号 Gene ID	log₂ (Fold Change)	P_adj-value	基因序列号 Gene ID	log₂ (Fold Change)	P_adj-value
zma-miRNA156b	2.1	1.65E-09	zma-miRNA156b	2.1	5.24E-05
zma-miRNA156i	2.8	3.09E-11	zma-miRNA156i	-2.3	6.64E-03
zma-miRNA156l	2.4	7.91E-05	zma-miRNA156l	-2.2	3.29E-04
zma-miRNA159a	-3.9	3.09E-33	zma-miRNA159a	1.6	3.23E-16
zma-miRNA159b	-4.0	1.27E-27	zma-miRNA159b	1.6	3.39E-16
zma-miRNA159c	-1.4	8.17E-04	zma-miRNA159c	3.8	1.19E-45
zma-miRNA159d	-1.4	3.35E-03	zma-miRNA159d	3.9	3.94E-44
zma-miRNA159f	-3.6	2.38E-30	zma-miRNA159f	1.8	6.23E-20
zma-miRNA159j	-4.2	8.07E-40	zma-miRNA159j	1.6	2.07E-16
zma-miRNA159k	-4.0	2.70E-31	zma-miRNA159k	1.6	6.04E-17
zma-miRNA166j	-2.5	5.90E-06	zma-miRNA166j	1.1	2.65E-02
zma-miRNA166k	-3.5	3.84E-12	zma-miRNA166k	1.0	2.91E-02
zma-miRNA166l	-2.8	1.07E-05	zma-miRNA166l	-1.4	4.30E-05
zma-miRNA167b	3.1	1.14E-07	zma-miRNA167b	-2.1	3.79E-10
zma-miRNA167c	2.4	2.38E-03	zma-miRNA167c	-1.1	2.00E-02
zma-miRNA167g	1.2	1.68E-03	zma-miRNA167g	-1.6	1.90E-04
zma-miRNA169g	10.8	3.70E-05	zma-miRNA169g	-4.3	2.80E-02
zma-miRNA171d	8.9	4.04E-02	zma-miRNA171d	-7.4	3.19E-02
zma-miRNA171g	1.9	4.27E-02	zma-miRNA171g	2.2	4.80E-02
zma-miRNA171l	8.9	4.04E-02	zma-miRNA171l	-8.9	1.00E-04
zma-miRNA171n	10.3	6.35E-04	zma-miRNA171n	-8.1	3.70E-03
zma-miRNA393b	12.4	4.92E-11	zma-miRNA393b	-4.1	4.03E-02
zma-miRNA396c	2.7	1.73E-12	zma-miRNA396c	-1.9	1.57E-14
zma-miRNA396d	2.2	1.84E-10	zma-miRNA396d	-1.9	1.03E-16
zma-miRNA398a	-1.9	6.31E-04	zma-miRNA398a	1.2	7.49E-04
zma-miRNA398b	-1.3	1.30E-03	zma-miRNA398b	1.1	2.59E-04
zma-miRNA408a	-2.0	1.01E-03	zma-miRNA408a	3.9	1.02E-80
zma-miRNA408b	-2.2	6.31E-04	zma-miRNA408b	3.9	5.01E-74
zma-miRNA2118a	-24.4	1.65E-06	zma-miRNA397b	-4.7	2.33E-94
zma-miRNA159e	-23.9	2.77E-06	zma-miRNA319d	2.8	1.86E-39
zma-miRNA399a	-23.9	2.77E-06	zma-miRNA319b	2.8	1.27E-35
zma-miRNA169a	-4.1	8.97E-06	zma-miRNA319a	2.2	5.98E-20
zma-miRNA166n	-3.3	5.31E-09	zma-miRNA319c	2.2	1.70E-16
zma-miRNA2118b	-2.5	1.72E-02	zma-miRNA390b	-3.9	7.90E-14
zma-miRNA166h	-2.5	1.46E-18	zma-miRNA171f	-10.0	1.12E-07
zma-miRNA166m	-2.4	4.24E-03	zma-miRNA169b	-2.7	1.34E-06
zma-miRNA166b	-2.4	1.35E-23	zma-miRNA390a	-3.3	1.43E-05
zma-miRNA166g	-2.3	3.18E-21	zma-miRNA1432	-3.1	1.68E-05
zma-miRNA166i	-2.2	4.79E-15	zma-miRNA164b	2.5	5.24E-05
zma-miRNA166d	-2.2	7.66E-14	zma-miRNA164c	2.6	5.42E-05
zma-miRNA166f	-2.1	2.65E-13	zma-miRNA164g	2.6	9.17E-04
zma-miRNA166a	-2.1	2.27E-13	zma-miRNA172d	-8.4	1.26E-03
zma-miRNA166c	-2.0	2.95E-15	zma-miRNA164a	2.3	3.02E-03
zma-miRNA166e	-1.9	9.86E-14	zma-miRNA167d	-1.7	3.19E-03
zma-miRNA528b	-1.7	1.40E-02	zma-miRNA397a	-4.9	3.70E-03
zma-miRNA528a	-1.5	1.49E-03	zma-miRNA164h	2.4	2.04E-02
zma-miRNA167i	1.1	2.66E-03	zma-miRNA399h	-7.4	3.13E-02
zma-miRNA167j	1.3	3.96E-02	zma-miRNA164d	2.1	3.55E-02
zma-miRNA167h	1.4	4.27E-02	zma-miRNA171m	-7.4	4.00E-02
zma-miRNA827	1.5	1.63E-03
zma-miRNA171h	1.5	2.78E-02
zma-miRNA167f	1.5	8.83E-03
zma-miRNA171k	1.6	2.07E-02
zma-miRNA156d	1.7	1.36E-03
zma-miRNA168a	1.7	1.07E-05
zma-miRNA156g	1.8	1.51E-12
zma-miRNA168b	1.8	7.65E-05
zma-miRNA396f	2.0	2.41E-02
zma-miRNA162	2.0	3.46E-03
zma-miRNA156e	2.1	1.46E-18
zma-miRNA156f	2.1	3.52E-04
zma-miRNA164e	2.3	1.57E-05
zma-miRNA156a	2.3	1.55E-10
zma-miRNA396g	2.3	1.34E-02
zma-miRNA156k	2.5	4.12E-09
zma-miRNA156h	2.7	9.32E-08
zma-miRNA156h	2.7	9.32E-08
zma-miRNA169r	9.2	2.47E-02
zma-miRNA395b	9.2	2.47E-02
zma-miRNA395p	9.2	2.07E-02
zma-miRNA171b	9.4	1.11E-02
zma-miRNA171e	9.8	3.46E-03
zma-miRNA399j	10.0	1.63E-03
zma-miRNA169k	10.2	8.17E-04
zma-miRNA171i	10.6	1.20E-04
zma-miRNA169m	11.0	8.17E-06
zma-miRNA172b	22.2	1.30E-05
zma-miRNA395o	22.2	1.30E-05

Table 2

Fig. 3

Fig. 4

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组织 Tissue	含量Content (mean±SD)		P值 P-value
组织 Tissue	CK (μg L^-1)	Hg (μg L^-1)	P值 P-value
B73地下部分Part below the ground of B73	26.79±0.21	28.41±0.09	1.07E-08^**
B73地上部分Part above the ground of B73	20.577±0.48	26.64±0.54	1.88E-06^**
郑58地下部分Part below the ground of Z58	20.29±0.82	28.25±0.04	1.86E-06^**
郑58地上部分Part above the ground of Z58	14.45±0.81	24.77±0.87	8.09E-06^**

Identification and validation of miRNA involved in mercury stress response in maize seedling roots

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