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Acta Agronomica Sinica ›› 2023, Vol. 49 ›› Issue (4): 917-925.doi: 10.3724/SP.J.1006.2023.24064

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Identification of SUMO protein family members and functional study of Bna.SUMO1.C08 gene in Brassica napus

CHEN Hui(), XIAO Qin, WANG Hua-Dong, WEN Jing, MA Chao-Zhi, TU Jin-Xing, SHEN Jin-Xiong, FU Ting-Dong, YI Bin*()   

  1. National Key Laboratory of Crop Genetic Improvement/National Engineering Research Center for Rapeseed/Huazhong Agricultural University, Wuhan 430070, Hubei, China
  • Received:2022-03-22 Accepted:2022-07-21 Online:2023-04-12 Published:2022-08-12
  • Contact: *E-mail: yibin@mail.hzau.edu.cn
  • Supported by:
    National Key Research and Development Program of China(2016YFD01008)

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Abstract:

Post-translational modifications are very important for protein function. SUMOylation is a very important post-translational modification, which has great influence on the key processes of plant growth and development. As an important oil and economic crops, there is few studies on SUMO modification in Brassica napus. To remedy this gap, SUMOylation in Brassica napus was investigated in this study. Firstly, 31 SUMO proteins were identified in Brassica napus by bioinformatics methods, which were divided into three groups (“canonical” SUMO, “non-canonical” SUMO, and SUMO-V). Secondly, the relative expression pattern indicated that, Bna.SUMO1.C08, a homologous gene of AtSUMO1, was highly expressed in roots, leaves, and silique. Subcellular localization revealed that Bna.SUMO1.C08 was localized in the nucleus and endoplasmic reticulum. Finally, Bna.SUMO1.C08 was overexpressed in Brassica napus and Bna.SUMO1.C08 could enhance plant resistance to PEG stress. This study laid a foundation for the subsequent research on SUMO modification in Brassica napus.

Key words: Brassica napus, SUMOylation, Bna.SUMO1.C08

Table S1

Basic information of SUMO protein family members in Brassica napus"

基因名
Gene name		 序列号
Sequence number		 基因长度
Gene length (bp)		 蛋白长度
Protein length (aa)		 分子量
Molecular weight (Da)		 等电点
Isoelectric point
Bna.SUMO1.C08 BnaC08T0170600ZS 304 101 11,083.39 4.91
Bna.SUMO1.A08 BnaA08T0169400ZS 304 101 11,124.43 4.91
Bna.SUMO1.C01 BnaC01T0209100ZS 316 105 11,589.98 4.93
Bna.SUMO1.A03 BnaA03T0494800ZS 304 101 11,123.50 4.91
Bna.SUMO1.A01 BnaA01T0162300ZS 303 100 11,120.40 4.93
Bna.SUMO1.C07 BnaC07T0472900ZS 295 98 10,889.23 4.91
Bna.SUMO1.A05 BnaA05T0335100ZS 259 86 10,309.86 10.61
Bna.SUMO1.C04 BnaC04T0237000ZS 256 85 9964.39 9.18
Bna.SUMO1.C06 BnaC06T0107300ZS 211 70 8117.07 9.25
Bna.SUMO1.C03 BnaC03T0634700ZS 211 70 8158.22 9.73
Bna.SUMO2.C09 BnaC09T0378000ZS 313 104 11,668.17 4.93
Bna.SUMO2.A10 BnaA10T0112600ZS 313 104 11,716.22 4.93
Bna.SUMO2.A02 BnaA02T0129200ZS 295 98 11,017.38 4.93
Bna.SUMO2.C02 BnaC02T0161400ZS 481 160 18,387.54 5.26
Bna.SUMO3.C03 BnaC03T0307000ZS 328 109 12,774.63 8.69
Bna.SUMO4.C07 BnaC07T0331100ZS 1213 404 46,871.13 9.15
Bna.SUMO4.A09 BnaA09T0050000ZS 244 81 9306.37 5.26
Bna.SUMO5.A04 BnaA04T0211000ZS 304 101 11,358.08 9.55
Bna.SUMO5.C03 BnaC03T0183400ZS 364 121 13,485.51 9.57
Bna.SUMO5.C04b BnaC04T0521300ZS 304 101 11,358.08 9.55
Bna.SUMO5.A05 BnaA05T0114400ZS 307 102 11,257.98 9.33
Bna.SUMO5.A03 BnaA03T0157500ZS 166 55 6198.18 9.52
Bna.SUMO5.A06 BnaA06T0124100ZS 325 108 12,451.37 8.66
Bna.SUMO5.C04c BnaC04T0143500ZS 295 98 10,885.65 9.61
Bna.SUMO5.C05 BnaC05T0152200ZS 313 104 11,998.78 8.69
Bna.SUMO5.C08 BnaC08T0244100ZS 292 97 11,140.70 5.04
Bna.SUMO5.C04a BnaC04T0143300ZS 307 102 11,279.03 9.73
Bna.SUMO6.A06 BnaA06T0362200ZS 1192 397 45,931.67 6.45
Bna.SUMO6.C09 BnaC09T0036100ZS 271 90 10,505.94 5.12
Bna.SUM-V.C06 BnaC06T0314000ZS 1042 347 38,854.96 5.23
Bna.SUM-V.A07 BnaA07T0276200ZS 724 241 27,254.87 5.32

Fig. 1

Sequence analysis of SUMO protein members in Brassica napus A: phylogenetic tree analysis. Group 1 in red is a “canonica” group. Group 2 in blue is a “non-canonica” group. Group 3 in purple is a sumo-related protein. B: the analyses of motif, domain, and gene structure. The first column is Motif; the second column is Domain; the third section is genetic structure. C: protein sequence alignment. The red cuboid is labeled as β-grasp domain range; dots are action sites."

Fig. 2

Chromosomal localization and collinearity analysis of SUMO protein gene in Brassica napus A: the chromosome location visualization of SUMO protein genes. B: the intra-species and interspecies collinearity analysis of Arabidopsis thaliana, Brassica oleracea, Brassica rapa, and Brassica napus."

Fig. 3

Relative expression pattern and subcellular localization of Bna.SUMO1.C08 genes A: the relative expression pattern. Bna.SUMO1.C08 is mainly expressed in roots, leaves, and scones. B: the subcellular localization. Bna.SUMO1.C08 locates in the nucleus and endoplasmic reticulum. Bar: 1 μm."

Fig. 4

Identification of Bna.SUMO1.C08 over expression transgenic lines at mRNA level A: the vector structure diagram of Bna.SUMO1.C08 overexpressed genes. B: the relative expression levels of Bna.SUMO1.C08 in overexpressed plants."

Fig. 5

Phenotypes of Bna.SUMO1.C08 over expression transgenic lines and wide type lines under PEG treatment A, B: the phenotype and statistics of root length. C, D: DAB staining and NBT staining. E, F: the enzyme activity detection of POD and SOD. *: P < 0.05; **: P < 0.01; ***: P < 0.001."

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