大豆百粒重稳定QTL qSW20-1定位及对产量和品质的影响

doi:10.3724/SP.J.1006.2023.34022

Abstract

Abstract:

Seed weight is one of the key factors of soybean yield. Cloning the key genes controlling seed weight in major quantitative trait loci (QTL) is of great significance to improve soybean yield. In this study, a high-density genetic linkage map was constructed by SLAF-seq using 325 recombinant inbred lines (RILs) constructed by Qihuang 34 × Dongsheng 16 as the experimental materials. The total map distance was 2945.26 cM, and the average map distance was 0.47 cM. Combined with three environmental 100-seed weight phenotypes, 11 QTL related to 100-seed weight were detected. Among them, the QTL with stable environment was qSW20-1, which explaining 9.73%-18.10% of phenotypic variation. The large grain allele of the QTL could significantly increase the number of seed per plant and seed weight per plant, but there was no adverse effect on the two quality traits of protein content and fat content. The interval size was 435.42 kb, containing 36 genes. Five candidate genes were predicted by gene annotation and expression pattern analysis. The results of this study laid a solid foundation for soybean yield-increasing gene mining and molecular design breeding.

Key words: soybean, 100-seed weight, SLAF map, QTL mapping

SUN Jian-Qiang, HONG Hui-Long, ZHANG Yong, GU Yong-Zhe, GAO Hua-Wei, ZHOU Ya, CAO Jie, QI Hang, ZHAO Quan, BAO Li-Gao, CHEN Qing-Shan, QIU Li-Juan. Mapping of stable QTL qSW20-1 for 100-seed weight and its effect on yield and quality in soybean[J].Acta Agronomica Sinica, 2023, 49(10): 2621-2632.

Figures/Tables 11

Table 1

Fig. 1

Fig. 2

Table 2

Fig. 3

Table 3

Table 4

Fig. 4

Fig. 5

Table S1

Gene annotation of genes in the location interval"

基因名称 Gene name	推测功能 Putative function
Glyma.20G114400	无注释 No annatation
Glyma.20G114500	二氢硫辛酰胺转乙酰酶 Dihydrolipoamide acetyltransferase
Glyma.20G114600	糖脂转移蛋白相关 Glycolipid transfer protein related
Glyma.20G114700	紫外线切除修复蛋白RAD23 UV excision repair protein RAD23
Glyma.20G114800	无注释 No annatation
Glyma.20G114900	无注释 No annatation
Glyma.20G114933	酸性N端SPT6 Acidic N-terminal SPT6
Glyma.20G114966	无注释 No annatation
Glyma.20G115000	PPR重复序列(PPR) PPR repeat (PPR)
Glyma.20G115100	Kow结构域转录因子1 Kow domain transcription factor 1
Glyma.20G115200	无注释 No annatation
Glyma.20G115300	DNA结合/转录因子 DNA binding/transcription factor
Glyma.20G115500	3-酮酰基辅酶a合酶5相关 3-ketoacyl-co a synthase 5
Glyma.20G115600	B-box锌指 B-box zinc finger
Glyma.20G115700	LOB结构域-包含蛋白42 LOB domain-containing protein 42
Glyma.20G115800	无注释 No annatation
Glyma.20G115900	无注释 No annatation
Glyma.20G116000	无注释 No annatation
Glyma.20G116100	50S核糖体蛋白L15, 叶绿体 50S ribosomal protein L15, chloroplast
Glyma.20G116200	锌指蛋白JAGGED相关 Zinc finger protein JAGGED related
Glyma.20G116300	蛋白质ISTR-1, 同功型A Protein ISTR-1, Isoform A
Glyma.20G116400	磷脂酶相关的//α/β-水解酶样蛋白 Phospholipase-related//α/β-hydrolase-like protein
Glyma.20G116500	腺苷酸异戊烯转移酶1, 叶绿体相关 Isopentene adenylate transferase 1, chloroplast correlation
Glyma.20G116600	VQ基序(VQ) VQ motif (VQ)
Glyma.20G116700	无注释 No annatation
Glyma.20G116800	单链核酸内切酶Single-stranded-nucleate endonuclease
Glyma.20G116900	酰转移酶 Acyltransferase
Glyma.20G117000	MYB样DNA结合蛋白MYB//MYB转录因子 MYB-like DNA binding protein MYB//MYB transcription factor
Glyma.20G117100	tRNA合成酶I类(E和Q), 催化结构域(tRNA-synt_1c) tRNA synthetases class I (E and Q), catalytic domain (tRNA-synt_1c)
Glyma.20G117150	谷氨酰胺-tRNA合成酶, 非特异性RNA结合区第1部分( tRNA_synt_1c_R1) Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1 (tRNA_synt_1c_R1)
Glyma.20G117200	无注释 No annatation
Glyma.20G117300	BAX抑制剂相关//未命名的亚家族 BAX inhibitor related//unnamed subfamily
Glyma.20G117400	无注释 No annatation
Glyma.20G117500	棉纤维表达蛋白(DUF761) Cotton fibre expressed protein (DUF761)
Glyma.20G117600	半胱氨酸-TRNA合成酶//半胱氨酸-TRNA连接酶 Cysteine-TRNA synthetase//cysteine-TRNA ligase
Glyma.20G117700	无注释 No annatation

Table S1

Fig. 6

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环境 Environment	亲本Parents		重组自交系RIL
环境 Environment	齐黄34 Qihuang 34	东生16 Dongsheng 16	均值 Mean	标准差 SD	最大值 Max.	最小值 Min.	峰度 Skewness	偏度 Kurtosis	变异系数 CV (%)
2021_CP	27.97±0.20	16.59±0.16	23.97	3.69	35.79	10.65	0.00	0.41	15.40
2022_SY	32.67±0.69	27.38±1.68	29.71	3.68	40.40	19.73	0.12	-0.15	12.40
2022_CP	29.48±2.22	22.63±1.29	25.64	2.78	35.30	16.58	0.77	0.09	10.83
BLUP	29.69±1.01	22.55±0.43	26.44	2.22	33.57	20.10	0.16	0.12	8.38

连锁群 Linkage group	标记数 No. of markers	总图距 Map distance (cM)	平均图距 Average map distance (cM)	间距<5 cM Gaps<5 cM (%)	最大间距 Max. gap (cM)
Gm01	348	145.08	0.42	100.00	4.54
Gm02	372	156.70	0.42	99.46	5.56
Gm03	365	167.92	0.46	99.18	7.46
Gm04	309	140.07	0.45	99.03	7.28
Gm05	183	160.41	0.88	98.35	5.38
Gm06	211	145.65	0.69	99.05	8.32
Gm07	371	161.22	0.44	99.46	9.49
Gm08	255	125.88	0.50	100.00	4.00
Gm09	357	133.83	0.38	99.72	5.50
Gm10	455	123.76	0.27	99.56	7.94
Gm11	131	122.39	0.94	96.92	10.95
Gm12	133	128.10	0.97	97.73	14.34
Gm13	243	125.73	0.52	99.17	10.69
Gm14	224	148.46	0.67	99.55	11.70
Gm15	625	167.77	0.27	99.36	7.90
Gm16	390	192.55	0.49	99.23	7.58
Gm17	345	148.67	0.43	98.84	6.76
Gm18	283	142.60	0.51	100.00	4.43
Gm19	484	152.16	0.32	99.79	6.31
Gm20	213	156.31	0.74	99.06	5.82
总计Total	6297	2945.26	0.47	99.17	14.34

位点 QTL	环境 Environment	染色体 Chr.	标记区间 Flanking marker	遗传区间 Genetic interval (cM)	标记物理位置(a4) Physical position of markers (a4)	阈值 LOD	表型贡献率 PVE (%)	加性效应 Add	已报道的区间 Reported QTLs
qSW6-1	2021_CP	6	Marker857319-Marker851862	43.5-44.5	28822155-35028781	4.18	4.01	-0.66 a	[29], [30]
qSW6-2	2022_SY	6	Marker870421-Marker829649	90.5-95.5	10214058-12141974	6.05	5.39	-0.80 a	[31]
qSW6-2	BLUP	6	Marker870421-Marker829649	90.5-95.5	10214058-12141974	3.61	3.23	-0.37 a	[31]
qSW7-1	2022_SY	7	Marker2815425-Marker2794476	91.5-94.5	6672130-7004632	5.61	5.03	0.76 b	[32]
qSW7-2	2022_CP	7	Marker2748563-Marker2730119	87.5-88.5	7479038-7542682	10.81	9.33	0.90 b	[32]
qSW12-1	2022_CP	12	Marker5062944-Marker4965351	49.5-50.5	18776850-21043651	3.71	3.06	0.51 b	新位点New
qSW13-1	2022_SY	13	Marker3590403-Marker3588064	85.5-87.5	36310106-36606816	5.74	5.05	-0.77 a	[33]
qSW15-1	2021_CP	15	Marker4129867-Marker3915380	28.5-29.5	24348758-26925946	5.50	5.62	0.77 b	[34]
qSW15-1	BLUP	15	Marker4129867-Marker3915380	28.5-29.5	24348758-26925946	7.30	6.89	0.53 b	[34]
qSW15-2	2022_SY	15	Marker4103818-Marker3854574	25.5-26.5	43842763-44130443	6.70	5.99	0.83 b	新位点New
qSW17-1	2021_CP	17	Marker5995186-Marker5989688	80.5-86.5	7982531-8957550	3.15	3.14	-0.57 a	[35]
qSW18-1	2022_CP	18	Marker4315158-Marker4223583	65.5-68.5	12642699-14065422	5.09	4.28	-0.61 a	新位点New
qSW20-1	2021_CP	20	Marker2521089-Marker2536819	38.5-39.5	35867412-36014827	13.78	14.26	-1.23 a	[36]
	2022_SY	20	Marker2377057-Marker2521089	36.5-38.5	35579408-36014827	18.06	18.10	-1.45 a	[36]
	2022_CP	20	Marker2377057-Marker2521089	35.5-38.5	35579408-36014827	10.85	9.73	-0.92 a	[36]
	BLUP	20	Marker2416012-Marker2448322	39.5-40.5	35916231-35920512	19.87	19.04	-0.89 a	[36]

Mapping of stable QTL qSW20-1 for 100-seed weight and its effect on yield and quality in soybean

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