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Acta Agron Sin ›› 2007, Vol. 33 ›› Issue (10): 1724-1728.

• RESEARCH NOTES • Previous Articles     Next Articles

Cloning of lyc-b Gene from Sweetpotato (Ipomoea batatas L.) and Transferring the Gene into Tobacco

CHEN Xuan-Yang 1,2 , YUAN Zhao-Nian 2, ZHANG Zhao-Jian 2, ZHENG Jin-Gui 1,*   

  1. 1 Institute of Agricultural Product Quality of Fujian Agricultural and Forestry University, 2 College of Crop Science of Fujian Agricultural and Forestry University, Fuzhou 350002, Fujiang, China)
  • Received:2006-08-30 Revised:1900-01-01 Online:2007-10-12 Published:2007-10-12
  • Contact: ZHENG Jin-Gui

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Abstract:

Lycopene β-cyclase, which catalyzes linear lycopene to cycle and become β-carotene, is one of key enzymes in the β-carotene biosynthesis pathway. Total RNA was purified from sweetpotato tuberous roots and was reverse-transcripted into cDNA, and the consensus sequence of the gene for lycopene β-cyclase (lyc-b) was amplified from the cDNA, then total cDNA sequence of lyc-b was obtained by the ways of Rapid Amplification of cDNA End (RACE). Sequence analysis showed that the complete sequence of lyc-b consists of 1 844 bp, which has an Open Reading Frame of 1 503 bp and encodes a protein of 501 amino acids. The gene was constructed into plant express vector p23-lyc-b，and was transferred into tobacco by Agrobacterium-mediated method. The results of PCR and Southern blot showed that the gene was integrated into tobacco genome.

Key words: Sweetpotato (Ipomoea batatas L.), Lycopeneβ-cyclase, Gene clone, Vector construction, Transformation

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