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Acta Agron Sin ›› 2008, Vol. 34 ›› Issue (12): 2112-2120.doi: 10.3724/SP.J.1006.2008.02112

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Analysis of Indica-Japonica Characteristics in Thermo-Sensitive Genic Male Sterile Rice Line Zhu 1S

LIU Ping1**,DAI Xiao-Jun1**,YANG Yuan-Zhu2,LI Wen-Jia1,OU Li-Jun1,LIANG Man-Zhong1,CHEN Liang-Bi1*   

  1. 1 College of Life Science, Hunan Normal University, Changsha 410081, Hunan; 2 Academy of Yahua Seeds Sciences, Changsha 410116, Hunan, China
  • Received:2008-03-31 Revised:2008-06-13 Online:2008-12-12 Published:2008-09-06
  • Contact: CHEN Liang-Bi

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Abstract:

Zhu 1S is an early indica thermo-sensitive genic male sterile (TGMS) rice line widely used in hybrid rice production. In this study, with typical indica and japonica cultivars as control, the DNA polymorphism of Zhu 1S was analyzed by using three molecular markers of ISSR, SRAP, and TRAP. The results showed that the nuclear genome of Zhu 1S was mainly indica-derived, but it contained some japonica-specific DNA fragments and had certain japonica consanguinity. The specific molecular fingerprint of Zhu 1S could be established by using any of the three molecular markers. Moreover, the analysis of ORF100, ORF29–TrnCGCA, TrnTUGU–TrnLUAA, intron sequences of rps16 gene in chloroplast DNA of Zhu 1S showed that the chloroplast genome of Zhu 1S was indica-type, while the chloroplast genomes of Pei’ai 64S and Zhun S were japonica-type. There were two base substitutions in TrnTUGU–TrnLUAA fragment of Zhu 1S. It should be applicable approach for breeding two-line early hybrid rice combination with high and stable yield by using dual-purpose genic male sterile lines with indica-type cytoplasm and appropriate japonica consanguinity in double-cropping rice area around the middle and lower reaches of the Yangtze river. Hence, the results have some instructions for the breeding of high quality dual-purpose genic male sterile lines and the rice combinations.

Key words: Thermo-sensitive genic male sterile, Molecular markers, Nuclear DNA, Chloroplast DNA

[1]Yuan L-P(袁隆平). Current status and developing prospects in two-line hybrid rice research in China. Res Agric Modernization (农业现代化研究), 1997, 18(1): 1–3 (in Chinese with English abstract)
[2]Yang Y-Z(杨远柱), Tang P-L(唐平徕), Yang W-C(杨文才). Breeding and utilization of TGMS line Zhu S in rice. Hybrid Rice (杂交水稻), 2000, 15(2): 6–9 (in Chinese with English abstract)
[3]Li B-H(李必湖), Wu H-X(吴厚雄), Xu M-L(徐孟亮), Liang M-Z(梁满中), Zhang Z-H(张振华), Chen L-B(陈良碧). Com-parative studies on the sensitivity of fertility of TGMS rice lines to low temperature of consecutive time. Acta Agron Sin (作物学报), 2003, 29(6): 930–936 (in Chinese with English abstract)
[4]Gu H-Y(顾红雅), Qu L-J(瞿礼嘉). Plant Molecular Biology—A Laboratory Manual (植物分子生物学——实验手册). Beijing: Higher Education Press, 1998. pp 1–7 (in Chinese)
[5]Li G C, Quiros F. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: Its application to mapping and gene tagging in Brassica. Theor Appl Genet, 2001, 103: 455–461
[6]Hu J, Vick B A. Target region amplification polymorphism: A novel marker technique for plant genotyping. Plant Mol Biol Rep, 2003, 21: 289–294
[7]Chen J J, Vick B A, January C C. Molecular mapping of a nu-clear male-sterility gene in sunflower (Helianthus annuus L.) using TRAP and SSR markers. Theor Appl Genet, 2006, 113: 122–127
[8]Cao Z-M(曹哲明), Zhang Z-W(张志伟), Zhou J-S(周劲松). De-tection of polymorphism in microsatellite sequences by TRAP and SSCP. Biotechnology (生物技术), 2005, 15(6): 22–24(in Chinese)
[9]Kanno A, Watanabe N, Nakamura I, Harai A. Variation in chloro-plast DNA from rice (Oryza sativa): Differences between dele-tions mediated by short direct-repeat sequences within a single species. Theor Appl Genet, 1993, 86: 579–584
[10]Sun C Q, Wang X K, Yoshimura A, Doi K. Genetic differentia-tion or nuclear, mitochondrial and chloroplast genomes in com-mon wild rice (Oryza rufipogon Griff.) and cultivated rice (Oryza sativa L.). Theor Appl Genet, 2002, 104: 1335–345
[11]Tang J B, Xia H A, Cao M L, Zhang X Q, Zeng W Y, Hu S N, Tong W, Wang J, Wang J, Yu J. A comparison of rice chloroplast genomes. Plant Physiol, 2004, 135: 412–420
[12]Shaw J, Lickey E B, Beck J T, Farmer S B, Liu W, Miller J, Siri-pun K C, Winder C T, Schilling E E, Small R L. The tortoise and the hare: II. Relative utility of 21 noncoding chloroplast DNA se- quences for phylogenetic analysis. Am J Bot, 2005, 92: 142–166
[13]Huang G-W(黄光文), Chen J-L(陈觉梁), Ou L-J(欧立军), Lu X-Y(卢向阳), Chen L-B(陈良碧). Comparison of chloroplast DNA between photoperiod-sensitive genic male sterile (PGMS) rice Nongken 58S and its derivative sterile lines. J Plant Physiol Mol Biol (植物生理与分子生物学学报), 2006, 32(3): 287–292 (in Chinese with English abstract)
[14]Yang Y-Z(杨远柱), Fu C-J(符辰建), Hu X-C(胡小淳), Zheng W-H(郑卫华). The major characteristics and cultivation tech-nique of the TGMS lines Zhu 1S and Lu 18S in rice. Crop Res (作物研究), 2005, 19(3): 156–159 (in Chinese with English ab-stract)
[15]Yang Y-Z(杨远柱), Tang P-L(唐平徕), Yang W-C(杨文才), Liu A-M(刘爱民), Chen Y-Q(陈运泉). Breeding and utilization of TGMS line Zhu 1S in rice. Hybrid Rice (杂交水稻), 2000, 15(2): 6–8(in Chinese with English abstract)
[16]Luo X-H(罗孝和), Qiu Z-Z(邱趾忠), Li R-H(李任华). Pei'ai 64S—A dual-purpose sterile line whose sterility is induced by low critical temperature. Hybrid Rice (杂交水稻), 1992, 7(1): 27–28(in Chinese with English abstract)
[17]Lu S-A(卢升安), Chen F-Y(陈芳远), Yi X-P(易小平). A study on the effect of cytoplasm gene of indica rice hybrid on combin-ing ability. J Guangxi Agric & Biol Sci (广西农业大学学报), 1993, 12(1): 1–5 (in Chinese with English abstract)
[18]Tang S-Z(汤述翥), Zhang Y-D(张亚东), Sun H-Q(孙红芹), Ye Q-B(叶全宝), Chen X-H(陈秀花), Gu M-H(顾铭洪). Effects of CMS cytoplasm in isonuclear alloplasmic CMS lines with wide compatibility in rice. Acta Agron Sin (作物学报), 2003, 29(2): 405–408 (in Chinese with English abstract)
[19]Mei Q-M(梅启明). Study on breeding of homokaryotic hetero-plasmic male sterile lines in rice. Hubei Agric Sci (湖北农业科学), 1998, (1): 7–10 (in Chinese with English abstract)
[20]Chen S-H(陈顺辉), Lu H-R(卢浩然), Yang J-B(杨聚宝). Studies on the utilization of intersubspecific heterosis in rice by two-line method—Analysis on cytoplasmic effects. J Fujian Agric Univ (福建农业大学学报), 1995, 24(2): 133–137 (in Chinese with English abstract)
[21]Tao D Y, Hu F Y, Yang J Y. Cytoplasm and cytoplasm-nucleus interactions affect agronomic traits in japonica rice. Euphytica, 2004, 135: 129–134
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