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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (11): 2055-2063.doi: 10.3724/SP.J.1006.2009.02055

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Relationship of Activities of Key Enzymes Involved in Nitrogen Metabolism with Nitrogen Utilization in Rice under Water-Nitrogen Interaction

SUN Yong-Jian,SUN Yuan-Yuan,LI Xu-Yi,GUO Xiang,MA Jun*   

  1. Rice Research Institute,Sichuan Agricultural University,Wenjiang 611130,China
  • Received:2009-06-15 Revised:2009-06-27 Online:2009-11-12 Published:2009-09-10
  • Contact: MA Jun, E-mail: majunp2002@163.com

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Abstract:

Hybrid rice Gangyou 527 was used to investigate the effects of three irrigation regimes (submerged irrigation, W1; dry cultivation, W3; and damp irrigation before booting stage plus shallow irrigation at booting stage plus wetting-drying alternation irrigation from heading stage to mature stage, W2) and different amounts ofN application onactivities of N metabolism enzymes and N absorption and utilization in rice, and the correlation of the N metabolism enzymes activities in functional leaves with N absorption and utilization and yield at different growth stages. The results showed that there was an obvious interaction between irrigation regime and amounts ofN application. Compared with other irrigation treatments, the treatment W2 promoted the N uptake from tillering to heading, N dry matter production efficiency (NMPE) and N production efficiency (NPE). W2 and suitable N application amount (180 kg ha-1) enhanced activities of N metabolism enzymes, yield, and nitrogen use efficiency, being the best model in this paper referred as the water-nitrogen coupling management model. Applying nitrogen 270 kg ha-1 resultedin negative effect of water-nitrogen interaction, slowing down the increase of activities of nitrate reductase (NR), glutamine synthetase (GS), and glutamate synthase (GOGAT), decreasing N agronomy efficiency (NAE), N recovery efficiency (NRE), and yield. Correlation analysis indicated that there existed significantly or highly significantly positive correlations of activities of N metabolism enzymes with indices of N uptake and utilization and yield, with different correlation coefficients of different growth stages. According to the conditions above, GS activity in function leaves might be a candidate indicator for N uptake and accumulation at different growth stages, and activities of NR, GS, GOGAT, and endopeptidase (EP) in flag leaves at heading stage for rice yield and NMPE, NPE, NAE, NRE.

Key words: Rice, Water-nitro gen interaction, Nitro gen metabolism, Enzyme Activities, Nitrogen utilization

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