Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (12): 2197-2204.doi: 10.3724/SP.J.1006.2009.02197

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Evaluating Uniformity and Stability of Wheat Cultivars Based on Ratio of Homozygous DNA Locus

WANG Li-Xin1,JI Wei1,LI Hong-Bo1,GE Ling-Ling1,Xin Ai-Hua1,WANG Li-Xia2,CHANG LLi-Fang,ZHAO Chang-Ping1*   

  1. 1Beijing Academy of Agricultural and Forestry Sciences/Beijing Engineering and Technique Research Center for Hybrid Wheat，Beijing 100097，China；2Institute of Biology Engineering，Inner Mongolia Agricultural University，Hohhot 010018，China
  • Received:2009-06-22 Revised:2009-07-15 Online:2009-12-10 Published:2009-10-13
  • Contact: ZHAO chang-ping, E-mail: bjhwc2003@yahoo.com.cn; Tel: 010-51503968

PDF

1667

Cited

16

Abstract:

Evaluation of the uniformity and stability is of great importance in the determination of cultivar release. The homozygous DNA locus ratio is one of the main factors affecting the uniformity and stability of wheat (Triticum aestivum L.) cultivars. To figure out the feasibility of molecular markers in testing the uniformity and stability of wheat cultivars based on the homozygous DNA locus ratio, we examined each 50 individuals of every line of 10 F4, 10 F5 lines and 511 cultivars with 172 pairs of SSR, 99 pairs of EST-SSR, and 76 pairs of AFLP-SCAR primers. The homozygous DNA locus ratio was 82.1–94.5% in the F4 lines and 95.7–99.4% in the F5 lines, and the predicted homozygous DNA locus ratio was 98–100% in the F6 generation according to the homozygous DNA locus ratio of 500 individuals from F5 lines. In the 136 released cultivars and 375 cultivars from the National Regional Trials, 10% were observed with the homozygous DNA locus ratio less than 95%, including 11 released cultivars. Thirteen out of 129 cultivars from the 2007–2008 National Regional Trial for Winter Wheat had the homozygous DNA locus ratio less than 95% according to the test result at 84 loci. Compared with the cultivars whose homozygous locus ratio was higher than 95% (control), eight of the 13 cultivars showed obviously segregation in major agronomic traits, such as plant height, plant type, spikelet number per spike, spike length, spike type and grain color, another five with 94% of the homozygous locus ratio had similar trait variations to the control. The test result of 375 cultivars from the National Regional Trials indicated that cultivars with the homozygous DNA locus ratio less than 90% did not have the uniformity and stability; whereas the most of cultivars with the homozygous DNA locus ratio higher than 95% and part of cultivars with the ratio ranging from 90% to 95% possessed the characteristics of uniformity and stability. The results indicated that molecular markers can be a great supplemental tool in the evaluation of uniformity and stability of new cultivars. Thereby, it is feasibly to be evaluated the uniformity and stability of wheat cultivars based on the homozygous DNA locus ratio. Fifty pairs of SSR, EST-SSR and AFLP-SCAR primers were recommended for testing the homozygous DNA locus ratio and at least two kind of molecular markers were advisable to be used in evaluating the uniformity and stability of wheat cultivars. In this study, the method and practical details were explained with the sample size of 20 individual plants and the test locus number of 50.

Key words: Common wheat, Homozygous DNA locus, Uniformity of varieties, Stability of varieties

[1]Tian L(田雷), Cao M-Q(曹鸣庆), Wang H(王辉), Guo J-X(郭晶心), Zhou N-Y(周乃元), Sun J(孙江), Jia X-H(贾希海). The application of AFLP markers in the reliability and seed purity test of corn cultivars. Seed World (种子世界), 2000, (11): 35-36
[2]Yan T-J(颜廷进), Li Q(李群), Li R-Y(李汝玉), Tan Z-X(谭振馨). The application of RAPD markers in purity test of corn cultivar. Shandong Agric Sci (山东农业科学), 2003, (1): 12-15
[3]Li R-Y(李汝玉), Li Q(李群), Tan Z-X(谭振馨), Yan T-J(颜廷进), Zhang W-L(张文兰), Song G-A(宋国安), Hou S-Z(侯淑贞). Purity testing method for maize hybrids using SSR markers. J Maize Sci (玉米科学), 2005, 13(1): 15-18 (in Chinese with English abstract)
[4]Wang F-G(王凤格), Zhao J-R(赵久然), Dai J-R(戴景瑞), Wang L(王璐), Yi H-M(易红梅), Guo J-L(郭景伦), Sun S-X(孙世贤), Liao Q(廖琴), Yang G-H(杨国航). Uniformity analysis of maize varieties by a set of SSR markers. Mol Plant Breed (分子植物育种), 2007, 5(1): 95-104 (in Chinese with English abstract)
[5]Sundaram R M, Naveenkumar B, Biradar S K, Balachandran S M, Mishra B, IlyasAhmed M, Viraktamath B C, Ramesha M. S, Sarma N. P. Identification of informative SSR markers capable of distinguishing hybrid rice parental lines and their utilization in seed purity assessment. Euphytica, 2008, 163: 215-224
[6]Singh R K, Sharma R K, Singh A K, Singh V P, Singh N K, Tiwari S P, Mohapatra T. Suitability of mapped sequence tagged microsatellite site markers for establishing distinctness, uniformity and stability in aromatic rice.Euphytica, 2004,135: 135-143
[7]Shi H-B(石海波), Wang L-X(王立新), Li H-B(李宏博), Zhang F-T(张风廷), Ma Q(马庆), Zhao C-P(赵昌平). Division of seeds motley and SSR loci impurity in wheat cultivar using SSR markers. Mol Plant Breed (分子植物育种), 2006, 4 (4): 513-519 (in Chinese with English abstract)
[8]Wang L-X(王立新), Chang L-F(常利芳),Ji W(季伟), Gao S-Q(高世庆), Zhao C-P(赵昌平). Impact of uniformity of seeds and DNA loci to wheat DNA fingerprinting. Nongchanpin Shichang Zhoukan (农产品市场周刊), 2008, (Crop Genet Breed Res—Triticeae special): 7-10 (in Chinese)
[9] Wang L-X (王立新), Chang L-F (常利芳), Li H-B (李宏博), Ge L-L (葛玲玲), Xin A-H (信爱华), Gao S-Q (高世庆), Ji W(季伟), Sun H (孙辉), Zhao C-P (赵昌平). Method of testing wheat seeds purity by molecular markers. J Triticeae Crops (麦类作物学报), 2009, 29(1): 1-8 (in Chinese with English abstract)
[10]De Riek J, Calsyn E, Everaert I, Van Bockstaele E, De Loose M. AFLP based alternatives for the assessment of distinctness, uniformity and stability of sugar beet varieties. Theor App Genet, 2001, 103: 1254-1265
[11] Cooke R J, Bredemeijer G M M, Ganal M W, Ganal M W, Peeters R, Isaac P, Rendell S, Jackson J, Röder M S, Korzun V, Wendehake K, Areshchenkova T, Dijcks M, Laborie D, Bertrand L, Vosman B. Assessment of the uniformity of wheat and tomato varieties at DNA microsatellite loci. Euphytica, 2003, 132: 331-341
[12] Guan R-X(关荣霞), Liu Y(刘燕), Liu Z-X(刘章雄), Chang R-Z(常汝镇), Qiu L-J(邱丽娟). Purity identification of soybean varieties with SSR technique. Mol Plant Breed (分子植物育种), 2003, 1(3): 357-360 (in Chinese with English abstract)
[13] Lu G-Y(陆光远), Wu X-M(伍晓明), Zhang D-X(张冬晓), Liu F-L(刘凤兰), Chen B-Y(陈碧云), Gao G-Z(高桂珍), Xu K(许鲲). SSR-based evaluation of distinctness and uniformity of rapeseed (Brassica napus L.) varieties under Chinese national official field tests. Sci Agric Sin (中国农业科学), 2008, 41(1): 32-42 (in Chinese with English abstract)
[14] Ji W(季伟), Wang L-X(王立新), Sun H(孙辉), Wang M-Y(王茅雁), Zhao C-P(赵昌平). Predigestion of wheat SSR analysis protocol. J Agric Biotechnol (农业生物技术学报), 2007, 15(5): 907-908
[15] Wang X-W(王晓维), Wang L-X(王立新), Chang L-F(常利芳), Wei J-M(魏建民), Zhao C-P(赵昌平). Exploitation and application of AFLP-SCAR markers in wheat. J Triticeae Crops (麦类作物学报), 2008, 28(5): 738-744 (in Chinese with English abstract)
[16]Wang L-X (王丽霞), Wang L-X(王立新),Ji W(季伟), Li H-B(李宏博), Xin A-H(信爱华), Chang L-F(常利芳), Wei J-M(魏健民), Zhao C-P(赵昌平). The homozygous DNA locus ratio and the variation range of agronomic traits of wheat F4 lines. Mol Plant Breed (分子植物育种), 2009, 74:(4): 703-708 (in Chinese with English abstract)
[1] JIN Yi-Rong, LIU Jin-Dong, LIU Cai-Yun, JIA De-Xin, LIU Peng, WANG Ya-Mei. Genome-wide association study of nitrogen use efficiency related traits in common wheat (Triticum aestivum L.) [J]. Acta Agronomica Sinica, 2021, 47(3): 394-404.
[2] ZHANG Ping-Ping,YAO Jin-Bao,WANG Hua-Dun,SONG Gui-Cheng,JIANG Peng,ZHANG Peng,MA Hong-Xiang. Soft wheat quality traits in Jiangsu province and their relationship with cookie making quality [J]. Acta Agronomica Sinica, 2020, 46(4): 491-502.
[3] Di JIN,Dong-Zhi WANG,Huan-Xue WANG,Run-Zhi LI,Shu-Lin CHEN,Wen-Long YANG,Ai-Min ZHANG,Dong-Cheng LIU,Ke-Hui ZHAN. Fine mapping and candidate gene analysis of awn inhibiting gene B2 in common wheat [J]. Acta Agronomica Sinica, 2019, 45(6): 807-817.
[4] Fang-Ping YANG,Jin-Dong LIU,Ying GUO,Ao-Lin JIA,Wei-E WEN,Kai-Xiang CHAO,Ling WU,Wei-Yun YUE,Ya-Chao DONG,Xian-Chun XIA. QTL mapping of adult-plant resistance to stripe rust in wheat variety holdfast [J]. Acta Agronomica Sinica, 2019, 45(12): 1832-1840.
[5] Yong-Jie MIAO, Jun YAN, De-Hui ZHAO, Yu-Bing TIAN, Jun-Liang YAN, Xian-Chun XIA, Yong ZHANG, Zhong-Hu HE. Relationship between Grain Filling Parameters and Grain Weight in Leading Wheat Cultivars in the Yellow and Huai Rivers Valley [J]. Acta Agronomica Sinica, 2018, 44(02): 260-267.
[6] XIAO Yong-Gui,Susanne DREISIGACKER,Claudia NU?EZ-RíOS,HU Wei-Guo,XIA Xian-Chun,HE Zhong-Hu. dsDNA Fluorescent Quantification and Genotyping in Common Wheat by FLUOstar System [J]. Acta Agron Sin, 2017, 43(07): 947-953.
[7] DONG Xue,LIU Meng,ZHAO Xian-Lin,FENG Yu-Mei,YANG Yan. Isolation and Characterization of LMW-GS Glu-A3 in Common Wheat Related Species [J]. Acta Agron Sin, 2017, 43(06): 829-838.
[8] LIU Kai,DENG Zhi-Ying,ZHANG Ying,WANG Fang-Fang,LIU Tong-Tong,LI Qing-Fang,SHAO Wen,ZHAO Bin,TIAN Ji-Chun*,CHEN Jian-Sheng*. Linkage Analysis and Genome-Wide Association Study of QTLs Controlling Stem-Breaking-Strength-Related Traits in Wheat [J]. Acta Agron Sin, 2017, 43(04): 483-495.
[9] GONG Xi,JIANG Yun-Feng,XU Bin-Jie,QIAO Yuan-Yuan,HUA Shi-Yu,WU Wang,MA Jian,ZHOU Xiao-Hong,QI Peng-Fei,LAN Xiu-Jin. Mapping QTLs for Awn Length in Recombinant Inbred Line Population Derived from the Cross between Common Wheat and Tibetan Semi-wild Wheat [J]. Acta Agron Sin, 2017, 43(04): 496-500.
[10] XU Wen,SHEN Hao,GUO Jun,YU Xiao-Cong,LI Xiang,YANG Yan-Hui,MA Xiao,ZHAO Shi-Jie,SONG Jian-Min. Drought Resistance of Wheat NILs with Different Cuticular Wax Contents in Flag Leaf [J]. Acta Agron Sin, 2016, 42(11): 1700-1707.
[11] WANG Xin,MA Ying-Xue,YANG Yang,WANG Dan-Feng,YIN Hui-Juan,WANG Hong-Gang. Identification of Dwarfing Wheat Germplasm SN224 and Analysis of QTLs for Its Agronomic Characters [J]. Acta Agron Sin, 2016, 42(08): 1134-1142.
[12] LIU Kai,DENG Zhi-Ying,LI Qing-Fang,ZHANG Ying,SUN Cai-Ling,TIAN Ji-Chun*,CHEN Jian-Sheng*. Mapping QTLs For Wheat Panicle Traits with High Density SNP Genetic Map [J]. Acta Agron Sin, 2016, 42(06): 820-831.
[13] ZHAO De-Hui,YAN Jun,HUANG Yu-Lian,XIA Xian-Chun,ZHANG Yan,TIAN Yu-Bing,HE Zhong-Hu,ZHANG Yong. Effect of 1BL/1RS Translocation on Gluten Protein Fraction Quantities and Dough Rheological Properties [J]. Acta Agron Sin, 2015, 41(11): 1648-1656.
[14] XIAO Yong-Gui, LI Si-Min, LI Fa-Ji, ZHANG Hong-Yan, CHEN Xin-Min, WANG De-Sen, XIA Xian-Chun, HE Zhong-Hu. Genetic Analysis of Yield and Physiological Traits in Elite Parent Jing 411 and Its Derivatives under Two Fertilization Environments [J]. Acta Agron Sin, 2015, 41(09): 1333-1342.
[15] ANG Li,HUANG Yu-Lian,CHANG Ping,YAN Jun,ZHANG Ye-Lun,XIA Xian-Chun,TIAN Yu-Bing,HE Zhong-Hu,ZHANG Yong. QTL Mapping for Arabinoxylans Content and Its Relationship with Processing Quality in Common Wheat [J]. Acta Agron Sin, 2014, 40(09): 1695-1701.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd