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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (5): 761-767.doi: 10.3724/SP.J.1006.2009.00761

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Identification and SSR Mapping of Two Powdery Mildew Resistance Genes in Wild Emmer (Triticum dicoccoides) Accessions IW3 and IW10

LI Gen-Qiao,FANG Ti-Lin**,ZHANG Hong-Tao,XIE Chao-Jie,YANG Zuo-Min,SUN Qi-Xin,LIu Zhi-Yong   

  1. Department of Plant Genetics & Breeding,China Agricultural University/State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis Research & Utilization, Ministry of Education, Beijing 100193,China
  • Received:2008-12-04 Revised:2009-02-17 Online:2009-05-12 Published:2009-03-20
  • Contact: LIU Zhi-Yong

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Abstract:

Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a major wheat disease in the world. Deployment of resistant varieties is considered the most economical and effective way for controlling the disease. Wild emmer is one of the important genetic resources for wheat disease resistance genes. Two wild emmer accessions, IW3 and IW10, collected from Mount Hermon, Israel, are highly resistant to prevailing Bgt isolate E09. Genetic analyses of the F2 populations and F3 progenies derived from the crosses between Triticum durum cultivar Langdon and IW3 or IW10 indicated that each accession possessed a single dominant gene, temporarily designated MlIW3 and MlIW10, respectively, conferred resistance to Bgt isolate E09. Bulked segregant analysis (BSA) and SSR mapping revealed that both MlIW3 and MlIW10 were flanked by SSR markers Xbarc84 and Xwmc326 with genetic distances of Xbarc84–4.6 cM–MlIW3–1.6 cM–Xwmc326 and Xbarc84–6.6 cM–MlIW10–0.6 cM–Xwmc326. Both MlIW3 and MlIW10 were physically mapped on the distal bin of chromosome 3BL using Chinese Spring nullisomic–tetrasomic, ditelisomic, and deletion lines. According to the collecting geographic sites of IW3 and IW10 in Israel and the SSR mapping data, MlIW3 and MlIW10 appear to be the same or allelic to wild emmer derived powdery mildew resistance gene Pm41 or in the same cluster with it.

Key words: Wild emmer, Powdery mildew resistance genes, SSR marker

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