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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (8): 1458-1461.doi: 10.3724/SP.J.1006.2009.01458

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

EST-Aided Conversion of AFLP Marker Linked to Dominant Male Sterility Gene in Brassica napus into SCAR

SONG Lai-Qiang1,2,YI Bin2,YANG Ming-Gui2,CHEN Lun-Lin1,FU Ting-Dong2   

  1. 1Key Laboratory of Oil Crops，jiangxi Academy of Agricultural Sciences,Nanchang 330200,China;2National Key Laboratory of Crop Genetic Improvement,National Subcenter of Rapeseed Improvement in Wuhan,Huazhong Agricultural University,Wuhan 430070,China
  • Received:2008-12-17 Revised:2009-03-08 Online:2009-08-12 Published:2009-05-19
  • About author:songlaiqiang@yahoo.com.cn

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Abstract:

Dominant genic male sterility (Ms) in Brassica napus has been widely utilized in recurrent selection and in heterosis application. Recent genetical studies verified that its restorer gene is an allele locating at the Ms gene locus. According to this genetic pattern, a whole sterile population (Msms) can be acquired by crossing a homozygous male sterile line (MsMs) with a temporary maintainer (msms) and further used as a female parent in hybrid production, but trans-breeding of the sterile line or the temporary maintainer line that has the same nuclear background with the temporary maintainer line or the sterile line is critical to obtain uniform hybrid population and to maintain heterosis. Because of being laborious and time-consuming, an AFLP marker is usually converted to a PCR marker which is more efficient in molecular marker-assisted selection. In present study, we developed a SCAR marker with bioinformatics method from an AFLP marker SA12MG14 tightly linked to the Ms. Homologous sequences for this marker were obtained through Blast search (http://www.ncbi.nlm.nih.gov), and a corresponding accession of EST from Brassica napus was found from the Arabidopsis thaliana Integrated Database (http://atidb.org/cgi-perl/gbrowse/atibrowse). According to the combined sequence information of the AFLP fragment and the EST, a pair of primers was designed and analyzed on a backcross population Popu2. A dominant SCAR marker S6B3 was successfully identified and further detected consistently on the population with the original AFLP marker. The detected band was clear and steady. This marker is 0.3 cM away from the Ms, and its practical application will enhance work efficiency of breeding for homozygous sterile lines homologous to corresponding temporary maintainers.

Key words: Brassica napus, Dominant genic male sterility, EST, AFLP, SCAR

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