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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (06): 887-894.doi: 10.3724/SP.J.1006.2010.00887

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Dense-Panicle-Related Gene Cloning from Rice Mutant A989 and Transgenic Plant Analysis

LI Ling1,3,SHI Zhen-Ying1,CHEN Ge-Zhi2,WANG Xin-Qi2,AN Lin-Sheng1,ZHANG Jing-Liu1*   

  1. 1National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology & Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China; 2The Plant Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; 3 School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
  • Received:2010-01-08 Revised:2010-03-04 Online:2010-06-12 Published:2010-04-20
  • Contact: ZHANG Jing-Liu,E-mail:jlzhang@sippe.ac.cn

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Abstract:

Rice (Oryza sativa L.) is a model monocotyledonous plant for genetic study due to its small genome size. Along with the completion of genome sequencing, gene cloning and function study becomes the most important task. The concomitant methodology of reverse genetics has played fundamental roles in identifying and studying rice genes in recent years. Rice heading time and inflorescence architecture are two inter-relating and agriculturally important characters. In Arabidopsis, TFL1 gene takes part in the configuration process and transition of growth stage, including flowering. In rice, there are four TFL1 gene homologies, RCN1-4, over-expression of any one of which could result in delayed flowering and abnormal inflorescence architecture. In this study, a mutant A989 with the character of dense panicle and late flowering was isolated from our T-DNA insertion population. Genetic and molecular analysis proved that in mutant A989, insertion of T-DNA nearby the RCN2 gene caused its over-expression, and resulted in the phenotype of dense panicle and late flowering. We further made RCN2 gene over-expressed driven by double 35S promoter, and analyzed characters of the transformants. Possible pathway of RCN2 gene function was discussed.

Key words: Rice, T-DNA, Dense panicle, RCN2

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