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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (08): 1409-1413.doi: 10.3724/SP.J.1006.2010.01409

• RESEARCH ACTIVITIES • Previous Articles     Next Articles

Development of Soybean Germplasm Lacking of 7S Globulin α-Subunit

LIU Shan-Shan,TENG Wei-Li**,ZHANG Bin-Bin,GE Yu-Jun,DIAO Gui-Zhu,ZHENG Tian-Hui,JIANG Zi-Qin,ZENG Rui,WU Shuai,LI Wen-Bin*   

  1. Soybean Research Institute,Northeast Agricultural University/Key Laboratory of Soybean Biology,Ministry of Education,Harbin 150030,China
  • Received:2010-01-04 Revised:2010-04-21 Online:2010-08-12 Published:2010-06-11
  • Contact: LI Wen-Bin, E-mail: wenbinli@yahoo.com

Abstract:

α-subunit of soybean β-conglycinin is one of the major allergens in soybean products. It is possible to reduce or remove the allergens from soybean by development of mutant lines. In this study, a cross was made between 10 major varieties in Heilongjiang province of China and “riB” lacking α'-, α-, and 11S acidic subunits in order to breed soybean variety characterized by α-subunit null. F1 seeds were sown individually and individual plants were harvested to obtain F2-progeny seeds. Subunit compositions of seeds in F1 and F2 were examined for storage protein by SDS-PAGE. The results showed that all the F1 seeds obtained from cross contained α'- and α-subunits. Five band types of 7S globulin in the F2-progeny were observed including α-null, (α+A1aA1bA2)-null, A3-null, (α'+A4)-null, and (α'+α)-null types.

Key words: Soybean, 7S globulin, α-subunit mull type

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