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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (08): 1409-1413.doi: 10.3724/SP.J.1006.2010.01409

• RESEARCH ACTIVITIES • Previous Articles     Next Articles

Development of Soybean Germplasm Lacking of 7S Globulin α-Subunit

LIU Shan-Shan,TENG Wei-Li**,ZHANG Bin-Bin,GE Yu-Jun,DIAO Gui-Zhu,ZHENG Tian-Hui,JIANG Zi-Qin,ZENG Rui,WU Shuai,LI Wen-Bin*   

  1. Soybean Research Institute,Northeast Agricultural University/Key Laboratory of Soybean Biology,Ministry of Education,Harbin 150030,China
  • Received:2010-01-04 Revised:2010-04-21 Online:2010-08-12 Published:2010-06-11
  • Contact: LI Wen-Bin, E-mail: wenbinli@yahoo.com

Abstract:

α-subunit of soybean β-conglycinin is one of the major allergens in soybean products. It is possible to reduce or remove the allergens from soybean by development of mutant lines. In this study, a cross was made between 10 major varieties in Heilongjiang province of China and “riB” lacking α'-, α-, and 11S acidic subunits in order to breed soybean variety characterized by α-subunit null. F1 seeds were sown individually and individual plants were harvested to obtain F2-progeny seeds. Subunit compositions of seeds in F1 and F2 were examined for storage protein by SDS-PAGE. The results showed that all the F1 seeds obtained from cross contained α'- and α-subunits. Five band types of 7S globulin in the F2-progeny were observed including α-null, (α+A1aA1bA2)-null, A3-null, (α'+A4)-null, and (α'+α)-null types.

Key words: Soybean, 7S globulin, α-subunit mull type

[1]Fukushina D. Recent progress of soybean protein foods. Food Rev Intl, 1991, (7): 323-325

[2]Yamaauchi F. Molecular understanding of soybean protein. Food Rev Intl, 1991, (7): 283-332

[3]Ladin B F, Doyle J J, Beachy R N. Molecular characterization of a detection mutation affecting the α′ subunit of β-conglycnin of soybean. J Mol Appl Genet, 1984, 2: 372-380

[4]Takahashi K, Banaba H, Kikuchi A, Ito M, Nakamura S. An induced mutant line lacking the α-subunit of β-conglycinin in soybean. Breed Sci, 1994, 46: 251-255

[5]Teraishi M, Takahashi M, Hajika M, Matsunaga R, Uematsu Y, Ishimoto M. Suppression of soybean β-conglycinin genes by a dominant gene, Scg-1[J].Theor Appl Genet

[6]Guan R-X(关荣霞), Chang R-Z(常汝镇), Qiu L-J(邱丽娟), Liu Z-X(刘章雄), Guo S-T(郭顺堂). Analysis of protein subunit 7S/11S constitution and allergen lacking of soybean (Glycine max L. Merrill) cultivars. Acta Agron Sin (作物学报), 2004, 30(11): 1076-1079 (in Chinese with English abstract)

[7]Ogawa T, Samoto M, Takahashi K. Soybean allergens and hypo-allergenic soybean products. Nutr Sci Vitam, 2000, 46: 271-279

[9]Samoto M, Fukuda Y, Takahashi K, Taruchi K, Hiemori M, Tsuji H, Ogawa T, Kawamura Y. Substantially complete removal of three major allergenic soybean proteins (Gly m Bd 30K, Gly m Bd 28, and the α-subunit of β-conglycinin) from soy protein by using a mutant soybean, Tohoku 124 [J].Biosci Biotech Biochem

[10]Ladin B F, Doyle J J, Beachy R N. Molecular characterization of a delection mutation affecting the α′ subunit of β-conglycnin of soybean. J Mol Appl Genet, 1984, 2: 372-380

[11]Hajika M, Takahashi M, Sakai S, Matsunaga R. Dominant inheritance of a trait lacking β-conglycinin detected in a wild soybean line. Breed Sci, 1998, 48: 383-386

[12]Kaizuma N, Kowata H, Odanaka H. Genetic variation on soybean seed proteins induced by irradiation. Rep Tohoku Br Crop Sci Soc Jpn, 1989, 32: 97-99

[13]Liu S-S(刘珊珊), Ohta K, Dong C P, Thanh V C, Ishimoto M, Qin Z W, Hirata Y. Genetic diversity of soybean (Glycine max L. Merrill) 7S globulin protein subunits. Genet Resour Crop Evol, 2006, 53: 1209-1219

[14]Hajika M, Takahashi M, Sakai S, Igita K. A new genotype of 7S globulin (β-conglycinin) detected in wild soybean (Glycine soja Sied. et. Zucc). Breed Sci, 1996, 46: 385-386

[15]Takahashi K, Mizuno Y, Yumoto S, Kitamura K, Nkamura S. Inheritance of the α-subunit deficiency of β-conglycinin in soybean (Glycine max L [J].Merrill) line induced by γ-ray irradiation. Breed Sci

[16]Takahashi K, Banba H, Kikuchi A, Ito M, Nakamura S. An induced mutant line lacking the α-subunit of β-conglycinin in soybean (Glycine max L. Merrill). Breed Sci, 1994, 44: 65-66

[17]Yagasaki K, Takagi T, Sakai M, Kitamura K. Biochemical characterization of soybean protein consisting of different subunits of glycinin [J].J Agric Food Chem

[18]Liu S-S(刘珊珊), Tian F-D(田福东), Gao L-H(高丽辉), Wang Z-K(王志坤), Ge Y-J(葛玉君), Diao G-Z(刁桂珠), Li W-B(李文滨). Effect of subunit composition of 7S globulin on soybean quality characteristics [J].Acta Agron Sin (作物学报.2008, 34(5):909-913

[19]Liu S-S(刘珊珊), Wang Z-K(王志坤), Ge Y-J(葛玉君), Diao G-Z(刁桂珠), Tian F-D(田福东), Gao L-H(高丽辉), Li W-B(李文滨). Correlations between relative content of individual subunit of 7S globulin and quality characteristics in soybean germplasm. Chin J Oil Crop Sci (中国油料作物学报), 2008, 30(3): 284-289 (in Chinese with English abstract)

[20]Wang Z-K(王志坤), Ge Y-J(葛玉君), Diao G-Z(刁桂珠), Li W-B(李文滨). Effect of subunit composition of 7S globulin on soybean quality characteristics. Acta Agron Sin (作物学报), 2008, 34(5): 1-5 (in Chinese with English abstract)

[21]Liu S-S(刘珊珊), Diao G-Z(刁桂珠), Wang Z-K(王志坤), Ge Y-J(葛玉君), Wu X-X(武小霞), Gao L-H(高丽辉), Tian F-D(田福东), Li W-B(李文滨). Characterization and evaluation for subun it composition of storage protein in soybean germplasm. Chin J Oil Crop Sci (中国油料作物学报), 2008, 30(4): 511-513 (in Chinese with English abstract)

[22]Yagasaki K, Kaizuma N, Kitamura K. Inheritance of glycinin subunits and characterization of glycinin molecules lacking the subunits in soybean

[Glycine max (L.) Merr]. Breed Sci, 1996, 46: 11-15

[23]Meng X-X(孟祥勋). Studies on storage protein in soybean seeds. J Northeast Agric Univ (东北农业大学学报), 1997, 28(2): 201-207 (in Chinese with English abstract)

[24]Kitamura K, Davies C S, Nielsen N C. Inheritance of alleles for Cgy1 and Gy4 storage in soybean [J].Theor Appl Genet

[25]Tsukada Y K, Kitamura K, Harada K. Genetia analysis of subunits of two major storage protein (β-conglycinin and glycinin) in soybean seeds. Jpn J Breed, 1986, 36: 390-400

[26]Chen S, Lin X H, Xu C G, Zhang Q F. Improvement of bacterial resistance of ‘Minghui 63’, an elite restorer line of hybrid rice, by molecular marker-assisted selection

[J].Crop Sci

[27]Wang X-W(王新望), Lai J-R(赖菁茹), Liu G-T(刘广田). Constructions of elite ph1b winter wheat lines and the application of the marker-assisted selection. Acta Agron Sin (作物学报), 2000, 26(3): 327-333 (in Chinese with English abstract)

[28]Xia J-H(夏军红), Zheng Y-L(郑用琏). Molecular marker- assisted backcross breeding of maize Rf3 NIL and its efficient analysis. Acta Agron Sin (作物学报), 2002, 28(3): 339-344 (in Chinese with English abstract)

[29]Cregan P B, Mudge J, Fickus E W, Danesh D, Denny R, Young N D. Two simple sequence repeat markers to select for soybean cyst nematode resistance conditioned by rhg1 locus. Theor Appl Genet, 2000, 99: 811-818

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