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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (3): 517-525.doi: 10.3724/SP.J.1006.2010.00517

• RESEARCH ACTIVITIES • Previous Articles     Next Articles

Cloning and Comparative Analysis of PURPLE ACID PHOSPHATASE 17 Gene Families in Brassica oleracea and Brassica rapa

LU Kun1,2,ZHANG Kai1,2,**,CHAI You-Rong1,2,LU Jun-Xing1,2,TANG Zhang-Lin1,2,*   

  1. 1 College of Agronomy and Life Sciences, Southwest University, Chongqing 400716, China; 2 Chongqing Rapeseed Technology Research Center, Chongqing 400716, China
  • Received:2009-09-18 Revised:2009-12-08 Online:2010-03-12 Published:2010-01-22
  • Contact: LI Jia-Na, E-mail: ljn1950@swu.edu.cn

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Abstract:

Two PAP17 genes were cloned from parent species of Brassica napus, B. oleracea and B. rapa, respectively. According to sequence similarity, PAP17 genes could be divided into two types, type I (BoPAP17-1 and BrPAP17-1) and type II (BoPAP17-2 and BrPAP17-2). Southern hybridization resulted in two bands both in B. oleracea and B. rapa, this is accordance with former cloning results. Phytogenetic and molecular evolution analysis indicated that PAP17 genes in Brassica species underwent purifying selection, and their deduced proteins are typical low molecular weight PAP proteins. Expression patterns of BoPAP17 and BrPAP17 genes were assayed by fluorescent quantitative PCR. The results revealed that PAP17 genes expressed in all nine tested tissues and organs, with the extremely high expression in flower and bud, and certain expression in seeds at different stages, implying these PAP17 genes most likely mobilize phosphorus reserves in plants, particularly during flower and bud development stages. Under phosphate starvation conditions, expression of BrPAP17-2 in seedling leaf was restrained, while that of BrPAP17 and BoPAP17 in seedling root and leaf was induced, the expression levels declined in the first 24 hours, and then continuously increased with the maximal levels between four days and eight days after treatment. After four days of Pi-resupply, their expression declined below un-induced basal levels. In comparison with seedling leaf, it seems that BrPAP17 and BoPAP17 showed stronger phosphate starvation induced expression in seedling root. These results thus suggested that PAP17 genes in B. oleracea and B. rapa may be involved in external phosphorus assimilation and transferring inorganic phosphate from root to other tissues or organs.

Key words: Brassica rapa, brassica oleracea, Purple acid phosphatase, Gene family, Phosphate starvation


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