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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (03): 459-468.doi: 10.3724/SP.J.1006.2011.00459

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Effects of SNP on AhSAG and AhBI-1 Genes Expression and Amelioration of Aluminum Stress to Peanut (Arachis hypoganea L.)

ZHAN Jie1,2,WANG Tian-Ju1,He Hu-Yi1,LI Chuang-Zhen1,HE Long-Fei1,*   

  1. 1 College of Agronomy, Guangxi University, Nanning 530004, China; 2 College of National Defense Education, Guangxi University, Nanning 530004, China
  • Received:2010-08-24 Revised:2010-12-03 Online:2011-03-12 Published:2011-01-17
  • Contact: 何龙飞, E-mail: lfhe@gxu.edu.cn

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Abstract: Recent researches have found that NO (nitric oxide) plays an important role in mediating some biotic and abiotic stress-induced oxidative stresses in plant kingdom. Meanwhile, Al toxicity is a major constraint for crop production in acidic soils worldwide, especially in southern China. In order to enucleate the control effects of NO (nitric oxide) on aluminum (Al) stress, we researched the effects of exogenous NO donor (sodium nitroprusside, SNP) on rootelongation and relative gene expression when Al-induced PCD in root tips happened with peanut (Arachis hypoganea L.) as material. The results of root elongation, hematoxylin dying and root tips Al3+ content detection showed that suitable exogenous NO could alleviate Al toxicity on root tips of peanut, especially at 1.0 mmol L–1 SNP. Compared with the control, SNP at 1.0 mmol L–1 increased root growth 45% and 52%, decreased Al3+ content of root tips 10% and 23% in 99-1507 and Zhonghua 2, respectively, in the meanwhile, the color of root tips with hematoxylin dying became light. Compared to Al stress only, relative expression of AhSAG gene in 99-1507(Al-resistant) increased firstly, than decreased after adding SNP at 1.0 mmol L–1, it decreased significantly in 0.1 and 0.4 mmol L–1 Al treatments, while that inZhonghua No.2 increased slowly, it increased significantlyin 0.1 and 0.4 mmol L–1 Al3+ treatments. Compared with Al stress only, relative expression of PCD-related AhBI-1 gene in 99-1507 increased firstly, than decreased after adding SNP at 1.0 mmol L–1, but that in Zhonghua 2 had a reverse change. The relative expression of AhBI-1 gene was lower in Al+SNP treatmentsthan in Al treatments only at 0 and 0.02 mmol L–1 in 99-1507, and at 0, 0.02, and 0.1 mmol L–1 in Zhonghua 2. The results showed that NO can alleviate Al toxicity in peanut, and it maybe relate to theregulationof the expression of programmed cell death related genes, while controls the programmed cell death.

Key words: Nitric Oxide, Peanut (Arachis hypoganea L.), Aluminum stress, Ameliorating effect, Gene expression

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