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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (04): 620-628.doi: 10.3724/SP.J.1006.2011.00620

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Expression Characteristic on TaPDC-E1a Gene and Its regulatory Enzymes Gene in Male Sterile Line of Wheat (Triticum aestivum)

ZHANG Long-Yu,YUAN Lei,YANG Shu-Ling,ZHANG Gai-Sheng*,WANG Jun-Sheng,SONG Yu-Long,ZHAO Zhuo-Jun,NIU Na,MA Shou-Cai   

  1. Northwest A&F University/Key Laboratory of Crop Heterosis of Shaanxi Province / Wheat Breeding Engineering Research Center, Ministry of Education, Yangling 712100, China
  • Received:2010-09-25 Revised:2011-01-06 Online:2011-04-12 Published:2011-02-24
  • Contact: 张改生, E-mail: zhanggsh@public.xa.sn.cn, Tel: 029-87092152

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Abstract: To deeply study the mechanism of male sterility induced by gametocide SQ-1 in wheat (Triticum aestivum L.), we isolated TaPDC-E1a gene using silcon cloning technique. The open reading frame of this gene is 1 401 bp in length, putatively encoding 388 amino acids. This gene possesses the conserved TPP domains. Two potential phosphorylation sites of serine residues might be present in the TaPDC-E1a protein of wheat. According to semi-quantitative RT-PCR analysis, the expression levels of TaPDC-E1a in the physiological and genetic male sterile lines were lower than those in fertile lines. Compared with fertile lines, the expression of PDK was obviously down-regulated in the physiological male sterile line induced by SQ-1. However, PDK gene was highly expressed in the genetic male sterile lines. The expression levels of PDP gene were similar in fertile and male-sterile lines. These results suggest that the pathway of energy metabolism of sterile line induced by SQ-1 is more susceptible than that of genetic male-sterile line.The upstream signal mechanism of mediating PDK gene may be inconsistent between male-sterile line induced bySQ-1 and genetic male-sterile line.

Key words: Wheat, gametocide SQ-1, TaPDC-E1a, PDK, PDP, Phosphorylation site

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