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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (04): 629-634.doi: 10.3724/SP.J.1006.2011.00629

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Gene Mapping of a Novel Mutant ahl in Rice

WANG Zeng**,LI Yun-Feng**,MA Jiao,REN De-Yong,WANG De-Zhong,YOU Xiao-Qing,SANG Xian-Chun,HE Guang-Hua*   

  1. Rice Research Institute, Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Southwest University, Chongqing 400716, China
  • Received:2010-09-17 Revised:2011-01-06 Online:2011-04-12 Published:2011-02-24
  • Contact: 何光华, E-mail: hegh@swu.edu.cn

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Abstract: Theresearch on rice flower development gene is very important in molecular breeding of rice for related traits. A rice flower mutant abnormal hull and lodicule (ahl) was identified from Jinhui 10 (Oryza sativa L. ssp. indica) treated by EMS. In ahl mutant, lemma and palea minished and significantly twisted along with the apparently elongated apical lodicules. This minished lemma and palea led toabnormal kernelsafter grain filling, meanwhile, the 1000-grain weight decreased. The trait wassteadily inherited, and controlled by a pair of recessive gene.Using bulked segregation analysis method AHL was restricted between SSR markers RM14153 and RM14167, with genetic distances of 1.34 cM and 1.19 cM respectively, which covered an approximate 226 kb region on the chromosome 2. The result is useful for further map-based cloning and functional analysis of AHL gene.

Key words: Rice (Oryza sativa L.), abnormal hull and lodicule, Genetic analysis, Gene mapping

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