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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (02): 210-214.doi: 10.3724/SP.J.1006.2012.00210


Analysis of Quantitative Trait Loci for Resistance to Brown Planthopper in Dongxiang Wild Rice (Oryza rufipogon Griff.)

HUANG De-Run,CHEN Jie,LAI Feng-Xiang,LIU Guang-Jie#,ZHUANG Jie-Yun*   

  1. State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China
  • Received:2011-07-25 Revised:2011-10-12 Online:2012-02-12 Published:2011-12-01
  • Contact: 庄杰云, E-mail: jz1803@hzcnc.com, Tel: 0571-63370369

Abstract: Wild species of Oryza is an important resource of the genes for resistance to brown planthopper (BPH). In this study, two populations were developed from an interspecific cross between Dongxiang wild rice (Oryza rufipogon Griff.) and cultivated rice Xieqingzao B (Oryza sativa L.) and used to determine quantitative trait loci (QTLs) for BPH resistance. A BC1F5 population derived from Xieqingzao B//Xieqingzao B/Dongxiang wild rice was infested with BPH collected from the paddy field. Two QTLs were detected, of which qBph2 was located in the interval RM29–RG157 on chromosome 2 and qBph7 in the interval RM11–RM234 on chromosome 7, and the Dongxiang wild rice allele decreased seedling mortality by 22.2% and 43.7%, respectively. Validation of the two QTLs was followed by testing a BC3F3 population with BPH biotypes I, II, and III, respectively. QTL analysis showed that qBph2 confers resistance to biotypes I and II, while qBph7 to biotypes I and III. The two QTLs have a great potential in the improvement of BPH resistance of rice varieties.

Key words: Dongxiang wild rice, Brown planthopper, Biotype, Quantitative trait locus

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