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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (11): 2052-2060.doi: 10.3724/SP.J.1006.2012.02052

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Differential Gene Expression Profiles in Developing Seeds of Brassica napus L. under Different Nitrogen Application Levels

YAN Gui-Xin,CHEN Bi-Yun,XU Kun,GAO Gui-Zhen,LÜ Pei-Jun,WU Xiao-Ming*,LI Feng,LI Jun   

  1. Oil Crops Research Institute, Chinese Academy of Agricultural Sciences / Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China
  • Received:2012-03-16 Revised:2012-06-10 Online:2012-11-12 Published:2012-09-10
  • Contact: 伍晓明, E-mail: wuxm@oilcrops.cn, Tel: 027-86812906

Abstract:

Nitrogen is one of the major fertilizers to Brassica napus L., it has concluded that increasing nitrogen would raise seed yield, enhance protein abundance but decrease oil content. Up-to-date, little is known on screening nitrogen-tolerant B. napus and identifying nitrogen-response genes and their networks. In the paper, we designed four nitrogen application levels (0, 90, 180, and 270 kg ha–1) using Brassica napus accessions Zhongshuang 11 and Parter (from Germany) with randomized block design. The results indicated that the seed protein content increased whereas oil content decreased with increasing nitrogen levels. There existed variance in protein and oil contents between Zhongshuang 11 and Parter. The comparison of 25 DAP seeds by Agilent oilseed microarrays at the whole-genome level between 180 kg ha–1 nitrogen fertilizer treatment and CK showed that Zhongshuang 11 contained 827 differential expressed genes, Parter comprised 3 676 differentially expressed genes, and two varieties jointly shared 278 differentially expressed genes, including 151 genes up-regulated and 80 genes down-regulated, and four genes with at least 10-fold difference expression. The functional classification of differentially expressed genes indicated that they mainly had binding, catalytic activities and transcriptional regulation activities. Most of them were involved in cellular, metabolic, and stimulative processes. The genes (about 50%) without function annotation should be studied further. Additionally, the expression of eight differentially expressed genes was validated by Quantitative RT-PCR. The results from e two methods were 94% consistent, which indicated that the microarray results were biologically reproducible. Our results shade light on exploring mechanism of nitrogen response and nitrogen-tolerance genotype identification in B. napus.

Key words: Microarray, Nitrogen fertilizer, Oil and protein content, Differentially expressed genes, Quantitative RT-PCR

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