Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (01): 118-125.doi: 10.3724/SP.J.1006.2013.00118

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Enhancing the Heterosis of Spring Rapeseed Varieties (Brassica napus L.) by Using Semi-Winter Rapeseed Varieties as Parents

AO Yan-Mei,LIU Hai-Dong,XU Liang,DU De-Zhi*   

  1. Spring Rapeseed Research Institute, Qinghai Academy of Agriculture and Forestry, Qinghai Sub-center of National Rapeseed Improvement, Qinghai provincial Key Laboratory Breeding Base for Innovation and Utilization of Plateau Crop Germplasm, Xining 810016, China
  • Received:2012-04-27 Revised:2012-10-09 Online:2013-01-12 Published:2012-11-14
  • Contact: 杜德志, E-mail: qhurape@126.com, 273545641@qq.com, Tel: 0971-5366520

Abstract:

Several B. napus varieties (lines) including two semi-winter rapeseed varieties, two spring restorer lines, two spring male-sterile lines and 16 spring restorer lines (derived from the spring restorer lines and semi-winter rapeseed varieties) were analyzed using SSR, SRAP and AFLP. Twenty-six combinations were produced according to the North Carolina mating design (NCII) by hand-pollinating 12 new restorer lines and one parental restorer line (Ag-5) with two spring male-sterile lines. The hybrid performance values were also determined. Among the 16 restorer lines, except for 931 and Zhang 23, the genetic distances were greater between the new restorer lines and two male-sterile lines than between the corresponding parental restorer line (Ag-5) and the two male-sterile lines, showing that introgressing semi-winter varieties into spring restorer lines could increase the genetic distance between spring restorer lines and spring male-sterile lines. The yield per plant for the maintainer lines of 15 combinations, which corresponded to the sterile lines showed high-parent values in 26 combinations, and 13 combinations showed stronger high-parent heterosis of yield per plant compared to combinations produced by the corresponding male-sterile and parental restorer lines (CMSL×Ag-5), suggesting that introgressing semi-winter varieties into spring restorer lines could enhance the heterosis of spring B. napus varieties Eighteen hybrids among the 24 combinations showed higher yield per plant compared to the combinations of CMSL× Ag-5, indicating introgressing semi-winter varieties into spring restorer lines might improve the spring B. napus hybrids yield. The results also showed that introgressing semi-winter varieties into spring restorer lines could improve the resistance to Sclerotinia sclerotiorum of spring B. napus hybrids. This study indicates that semi-winter B. napus rapeseed may be a valuable source of germplasm for spring hybrid breeding.

Key words: Spring rapeseed, CMS lines, Restorer lines, Molecular markers, Heterosis, Sclerotinia sclerotiorum

[1]Ma C-Z(马朝芝), Fu T-D(傅廷栋), Tuevesson S, Gertsson B. Genetic diversity of chinese and Swedish rapeseed (Brassica napus L.) analysed by inter-simple sequence repeats (ISSRs). Sci Agrci Sin (中国农业科学), 2003, 36(11): 1403–1408 (in Chinese with English abstract)



[2]Charter Y M, Robertson A, Wilkinson M J, Ramsay G. PCR analysis of oilseed rape cultivars (Brassica napus L. ssp. oleifera) using 5'-anchored simple sequence repeat (SSR) primers. Theor Appl Genet, 1995, 92: 442–447



[3]Qian W, Meng J, Li M, Frauen M, Sass O, Noack J, Jung C. Introgression of genomic components from Chinese Brassica rapa contributes to widening the genetic diversity in rapeseed (B. napus L.) with emphasis on the evolution of Chinese rapeseed. Theor Appl Genet, 2006, 113: 49–54



[4]Du D-Z(杜德志), Nie P(聂平), Xu L(徐亮), Luo Y-X(罗玉秀), Yao Y-M(姚艳梅), Zhou H-W(周红伟), Zhang X-M(张晓梅). Rapeseed heterosis of different ecotypes in Qinghai province. Chin J Oil Crop Sci (中国油料作物学报), 2010, 32(2): 180–186 (in Chinese with English abstract)



[5]Yao Y-M(姚艳梅). Comparative of several Different traits in different ecotypes of Brassica napus L. J Qinghai Univ (Nat Sci) (青海大学学报•自然科学版), 2011, 29(6): 2–4 (in Chinese with English abstract)



[6]Doyle J J, Doyle J L. Isolation of plant DNA from fresh tissue. Theor Appl Genet, 1990, 12: 13–15



[7]Tautz D. Hypervariability of simple sequence as general source for polymorphic DNA markers. Nucl Acids Res, 1989, 12: 6463–6467



[8]Li G, Quiros C F. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and genetagging in Brassica. Theor Appl Genet, 2001, 103: 455–461



[9]Vos P, Hogers R, Bleeker M, Reijans M, Van de Lee T, Hornes M, Freijters A, Pot J, Peleman J, Kuiper M, Zabeau M. AFLP: a new technique for DNA fingerprinting. Nucl Acids Res, 1995, 23: 4407–4414



[10]Zhao J W, Meng J L. Genetic analysis of loci associated with partial resistance to sclerotinia sclerotiorum in rapeseed (Brassica napus L.). TAG Theor Appl Genet, 2003, 106: 759–764



[11]Luo K(罗宽), Zhou B-W(周必文). Disease and Prevention of Rapeseed (油菜病害及其治理). Beijing: China Business Press, 1994 (in Chinese)



[12]Ran Y(冉毅), Wen C-J(文成敬), Niu Y-Z(牛应泽). Comparison ofmethods for identification of resistance to Scleroti sclerotiorum and screening of resistant materials in rapeseed. J Plant Prot (植物保护学报), 2007, 34(6): 601–606 (in Chinese with English abstract)



[13]Nei M, Li W. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA, 1979, 76: 5256–5273



[14]Sneath P H A, Sokal R R. Numerical Taxonomy. San Francisco: WH Freeman, 1973



[15]Rohlf F J. NTSYS-pc Numerical Taxonomy and Multivariate Analysis System, vertion 1.80. New York: Exeter Publication, 1990.



[16]Zhang T-Z(张天真). Pandect of Crop Breeding (作物育种学总论). Beijing: China Agriculture Press, 2003. pp 146–149 (in Chinese)



[17]Tang Q-Y(唐启义), Feng M-G(冯明光). Utility Statistics Analysis and Data Processing System (实用统计分析及其DPS数据处理系统). Beijing: Science Press, 2002. pp 333–339, 367–373 (in Chinese)



[18]U N. Genomic analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization. Jpn J Bot, 1935, 7: 389–452



[19]Liu H. Genetics and Breeding in Rapeseed. Beijing: China Agricultural University Press, 2000. pp 20–23, 26–45



[20]Sun V G. The evaluation of taxonomic characters of cultivated Brassica with a key to species and variances. 1. The characters. Bull Torrey Bot, 1946, 73: 244–281



[21]Denford K E, Vaughan J G. A comparative study of certain seed isoenzymes in the ten chromosome complex of Brassica campestris and its allies. Ann Bot, 1977, 41: 411–418



[22]Qian W, Liu R, Meng J. Genetic effects on biomass yield in interspecific hybrids between Brassica napus and Brassica rapa. Euphytica, 2003, 134: 9–15



[23]Song K M, Osborn T C, Williams P H. Brassica taxonomy based on nuclear restriction fragment length polymorphism (RFLP) 2. Preliminary analysis of subspecies within B. rapa. Theor Appl Genet, 1988, 76: 593–600



[24]Zhao J-Y(赵坚义), Becker H C. Genetic variation in Chinese and European oilseed rape (B. napus) and turnip rape (B. campestris) analysis with isozymes. Acta Agron Sin (作物学报), 1998, 24(2): 213–220 (in Chinese with English abstract)



[25]Zhao J, Wang X, Deng B, Lou P, Wu J, Sun R, Xu Z, Vromans J, Koornneef M, Bonnema G. Genetic relationship within Brassica rapa as inferred from AFLP fingerprints. Theor Appl Genet, 2005, 110: 1301–1314



[26]Udall JA, Quijada PA, Polewicz H, Vogelzang R D, Osborn T C. Phenotypic effects of introducing unadapted germplasm into a spring canola hybrid. Crop Sci, 2004, 44: 1990–1996



[27]Quijada P A, Udall JA, Polewicz H, Vogelzang R D, Osborn T C. Phenotypic effects of introgressing French winter germplasm into hybrid spring canola (Brassica napus L.). Crop Sci, 2004, 44: 1982–1989



[28]Butruille D V, Guries R P, Osborn T C. Increasing yield of spring oilseed rape hybrids (Brassica napus L.) through introgression of winter germplasm. Crop Sci, 1999, 39: 1491–1496



[29]Fei W-X(费维新), Li Q-S(李强生), Chen F-X(陈凤祥), Zhang Y(张跃), Wu X-J(吴新杰), Hou S-M(侯树敏), Jiang Y-F(江莹芬), Hu B-C(胡宝成). Preliminary report on the resistance to Sclerotinia sclerotiorum of 14 varieties of Brassica napus L. Chin Sci Agric Bull (中国农学通报), 2007, 23(1): 254–257 (in Chinese with English abstract)



[30]Liu C-Q(刘澄清), Du D-Z(杜德志), Huang Y-J(黄有菊), Wang C-H(王春华). Study on the resistant to disease and genetic effects of varieties of Brassica napus L. Sci Agrci Sin (中国农业科学), 1991, 24(3): 43–49 (in Chinese with English abstract)

[1] Wang Yue-Sheng, Ge Dong-Dong, Cheng Lan-Fei, Chen Chun-Huan, Wang Chang-You, Liu Xin-Lun, Li Ting-Dong, Deng Ping-Chuan, Ji Wan-Quan, Zhao Ji-Xin. Molecular cytogenetic and disease resistance characterization of the wheat- Psathyrostachys huashanica disomic substitution line 16DH25-7 [J]. Acta Agronomica Sinica, 2026, 52(2): 433-445.
[2] ZHANG Jin-Ze, ZHOU Qing-Guo, YANG Xu, WANG Qian, XIAO Li-Jing, JIN Hai-Run, OU-YANG Qing-Jing, YU Kun-Jiang, TIAN En-Tang. Analysis of genes associated with expression characteristics and high resistance in response to Sclerotinia sclerotiorum infection in Brassica juncea [J]. Acta Agronomica Sinica, 2025, 51(3): 621-631.
[3] TIAN Han-Zhao, FENG Long-Ting, YING Kai, MENG Tian-Qi, WU Jun, LIU Yu-Xiu. Composition and evaluation of wheat glutenin subunits in exotic wheat germplasm [J]. Acta Agronomica Sinica, 2025, 51(10): 2663-2680.
[4] SONG Meng-Yuan, GUO Zhong-Xiao, SU Yu-Fei, DENG Kun-Peng, LAN Tian-Jiao, CHENG Yu-Xin, BAO Shu-Ying, WANG Gui-Fang, DOU Jin-Guang, JIANG Ze-Kai, WANG Ming-Hai, XU Ning. Transcriptome analysis of a stigma exsertion mutant in mungbean [J]. Acta Agronomica Sinica, 2024, 50(4): 957-968.
[5] SONG Zhao-Jian, FENG Zi-Yi, QU Tian-Ge, LYU Pin-Cang, YANG Xiao-Lu, ZHAN Ming-Yue, ZHANG Xian-Hua, HE Yu-Chi, LIU Yu-Hua, CAI De-Tian. Indica-japonica attribute identification and heterosis utilization of diploid rice lines reverted from tetraploid rice [J]. Acta Agronomica Sinica, 2023, 49(8): 2039-2050.
[6] SU Zai-Xing, HUANG Zhong-Qin, GAO Run-Fei, ZHU Xue-Cheng, WANG Bo, CHANG Yong, LI Xiao-Shan, DING Zhen-Qian, YI Yuan. Identification of wheat dwarf mutant Xu1801 and analysis of its dwarfing effect [J]. Acta Agronomica Sinica, 2023, 49(8): 2133-2143.
[7] YANG Yi-Dan, HE Du, LIU Jing, ZHANG Yan, CHEN Fei-Zhi, WU Yan-Fei, DU Xue-Zhu. Application of host-induced gene silencing interfering with Sclerotinia sclerotiorum pathogenic gene OAH in Brassica napus resistance to Sclerotinia sclerotiorum [J]. Acta Agronomica Sinica, 2023, 49(6): 1542-1550.
[8] YANG Xiao-Ming, CHENG Xu-Zhen, ZHU Zhen-Dong, LIU Chang-Yan, CHEN Xin. Advances in germplasm innovation and genetic improvement of food legumes resistant to bruchid [J]. Acta Agronomica Sinica, 2023, 49(5): 1153-1169.
[9] ZHU Zhi, LI Long, LI Chao-Nan, MAO Xin-Guo, HAO Chen-Yang, ZHU Ting, WANG Jing-Yi, CHANG Jian-Zhong, JING Rui-Lian. Transcription factor TaMYB5-3B is associated with plant height and 1000- grain weight in wheat [J]. Acta Agronomica Sinica, 2023, 49(4): 906-916.
[10] CHEN Bing-Ru, YU Miao, GE Zhan-Yu, LI Hong-Kui, HUANG Yan, LI Hai-Qing, SHI Gui-Shan, XIE Li, XU Ning, YAN Feng, GAO Shi-Jie, ZHOU Zi-Yang, WANG Nai. Analysis of heterotic groups and heterosis patterns of sorghum in early- maturing area [J]. Acta Agronomica Sinica, 2023, 49(2): 343-353.
[11] HUI Zhi-Ming, XU Jian-Fei, JIAN Yin-Qiao, BIAN Chun-Song, DUAN Shao-Guang, HU Jun, LI Guang-Cun, JIN Li-Ping. 2b-RAD based maturity associated molecular marker identification in tetraploid potato (Solanum tuberosum L.) [J]. Acta Agronomica Sinica, 2022, 48(9): 2274-2284.
[12] CHEN Song-Yu, DING Yi-Juan, SUN Jun-Ming, HUANG Deng-Wen, YANG Nan, DAI Yu-Han, WAN Hua-Fang, QIAN Wei. Genome-wide identification of BnCNGC and the gene expression analysis in Brassica napus challenged with Sclerotinia sclerotiorum and PEG-simulated drought [J]. Acta Agronomica Sinica, 2022, 48(6): 1357-1371.
[13] SHI Yu-Qin, SUN Meng-Dan, CHEN Fan, CHENG Hong-Tao, HU Xue-Zhi, FU Li, HU Qiong, MEI De-Sheng, LI Chao. Genome editing of BnMLO6 gene by CRISPR/Cas9 for the improvement of disease resistance in Brassica napus L [J]. Acta Agronomica Sinica, 2022, 48(4): 801-811.
[14] WANG Heng-Bo, CHEN Shu-Qi, GUO Jin-Long, QUE You-Xiong. Molecular detection of G1 marker for orange rust resistance and analysis of candidate resistance WAK gene in sugarcane [J]. Acta Agronomica Sinica, 2021, 47(4): 577-586.
[15] GUO Yan-Chun, ZHANG Li-Lan, CHEN Si-Yuan, QI Jian-Min, FANG Ping-Ping, TAO Ai-Fen, ZHANG Lie-Mei, ZHANG Li-Wu. Establishment of DNA molecular fingerprint of applied core germplasm in jute (Corchorus spp.) [J]. Acta Agronomica Sinica, 2021, 47(1): 80-93.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!