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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (07): 1214-1222.doi: 10.3724/SP.J.1006.2013.01214

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Analysis of QTLs for Oil, Protein, Cellulose and Hemicellulose Contents of Seeds in Brassica napus L.

MA Zhen-Zhen1,2,LI Jia-Na1,2,Benjiamin WITTKOP3,Martin FRAUEN4,YAN Xing-Ying1,2,LIU Lie-Zhao1,2,*,XIAO Yang1,2   

  1. 1 College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China; 2 Chongqing Engineering Research Center for Rapeseed, Chongqing 400716, China; 3 Department of Plant Breeding, IFZ Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany; 4 Norddeutsche Pflanzenzucht Hans-Georg Lembke KG, 24363 Hohenlieth, Germany
  • Received:2012-11-16 Revised:2013-03-11 Online:2013-07-12 Published:2013-04-23
  • Contact: 刘列钊, E-mail: liezhao2003@126.com, Tel: 023-68250701

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Abstract:

In order to study oil, protein, cellulose and hemicellulose contents of rapeseed, recombinant inbred lines (RILs) were developed through successive selfing up to eight generations from the F2 of a cross between yellow seeded female parent GH06 and black seeded male parent P174. The SSR markers were used to construct the linkage map, containing 31 linkage groups with the total map length of 1437.1 cM and an average distance of 3.89 cM. We measured the RIL population seeds quality in four different environments, the result showed that the oil content was negatively correlated with other three traits, and there were a negative correlation between protein content and cellulose content and a positive correlation between protein content and hemicellulose content. Using the method of composite interval mapping (CIM) to analysis oil, protein, cellulose and hemicellulose contents, a total of 26 QTLs for affecting the four traits were detected in linkage groups of N2, N3, N8, N9, N11, N13, N16, and N17. Eight QTLs were detected for oil content and the contribution for phenotypic variation ranged from 4.96% to 21.83%, six QTLs were detected for protein content and the contribution for phenotypic variation ranged from 3.12% to 14.28%, four QTLs were detected for cellulose content and the contribution for the phenotypic variation ranged from 4.60% to 17.29%, eight QTLs were detected for hemicellulose content and the contribution for phenotypic variation ranged from 6.66% to 16.68%. Furthermore, overlapping regions were observed in linkage group N8 between QTLs of oil content and QTLs for hemicellulose content, andin linkage group N9 between QTLs of cellulose content and QTLs for hemicellulose content. These results suggested that oil, protein, cellulose and hemicellulose contents are typical quantitative traits. They are controlled by many minor effect genes and influenced by environment. Molecular markers linked with these QTLs will be useful for facilitating molecular marker-assisted breeding.

Key words: Brassica napus L., Oil content, Protein, Cellulose, Hemicellulose, QTL

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