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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (08): 1391-1399.doi: 10.3724/SP.J.1006.2013.01391

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Expression Analysis of GbMFT1 Gene in Gossypium barbadense L.

GU Chao1,LI Chao1,LI Xiao-Bo2,XIAO Xiang-Wen2,CUI Bai-Ming1,HUANG Xian-Zhong1,*   

  1. 1 Key Laboratory of Agrobiotechnology, College of Life Sciences, Shihezi University, Shihezi 832003, China; 2 Key Laboratory of Chemistry of Plant Resources in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China
  • Received:2013-01-29 Revised:2013-04-22 Online:2013-08-12 Published:2013-05-20
  • Contact: 黄先忠, E-mail: xianzhongh106@163.com, Tel: 0993-2057262

Abstract:

One MFT (MOTHER OF FT AND TFL1)-like homolog was isolated from Gossypium barbadense L. cv. Xinhai14 through RT-PCR and RACE method, which was designated as GbMFT1 gene. The open reading frame (ORF) of GbMFT1 is 528 bp in length, encoding a putative protein of 175 amino acids. GbMFT1 gene has a phosphatidylethanolamin-binding protein (PEBP) conserved motif. Liking almost all MFT-like proteins, GbMFT1 protein has a conserved proline residue near the C-terminus. Phylogenetic analysis revealed that GbMFT1 showed closer kinship with VvMFT and SP2G, indicating that they belong to the same evolutionary branch. Expression analysis by qRT-PCR indicated that GbMFT1 displayed a much broader expression range, with a maximum expression in petal. GbMFT1 was also expressed in different developmental stages of cotton fibres, especially in the two DPA ovules and nine DPA fibres. Expression analysis by semi-quantitative method indicated that GbMFT1 had a high expression level at the begining of seed germination. There existed no significant difference of expression in germinating seeds treated with different concentrations of ABA, indicating the expression of GbMFT1 is not regulated by ABA. Our results lay a good foundation in studying the function of cotton GbMFT1 gene further.

Key words: GbMFT1 gene, Early flowering, Gene expression, Florigen

[1]Bradley D, Carpenter R, Copsey L, Vincent C, Rothstein S, Coen E. Control of in?orescence architecture in Antirrhinum. Nature, 1996, 379: 791–797

[2]Bradley D, Ratcliffe O, Vincent C, Carpenter R, Coen E. In?orescence commitment and architecture in Arabidopsis. Science, 1997, 275: 80–83

[3]Kardailsky I, Shukla V, Ahn J, Dagenais N, Christensen S, Nguyen J, Chory J, Harrison M, Weigel D. Activation tagging of the ?oral inducer FT. Science, 1999, 286: 1962–1965

[4]Kobayashi Y, Kaya H, Goto K, Iwabuchi M, Araki T. A pair of related genes with antagonistic roles in mediating ?owering signals. Science, 1999, 286: 1960–1962

[5]Hanzawa Y, Money T, Bradley D. A single amino acid converts a repressor to an activator of flowering. Proc Natl Acad Sci USA, 2005, 102: 7748–7753

[6]Ahn J H, Miller D, Winter V J, Banfield M J, Lee J H, Yoo S Y, Henz S R , Brady R L, Weigel D. A divergent external loop confers antagonistic activity on floral regulators FT and TFL1. EMBO J, 2006, 25: 605–614

[7]Ohshima S, Murata M, Sakamoto W, Ogura Y, Motoyoshi F. Cloning and molecular analysis of the Arabidopsis gene Terminal Flower 1. Mol Gen Genet, 1997, 254: 186–194

[8]Mimida N, Goto K, Kobayashi Y, Araki T, Ahn J H, Weigel D, Murata M, Motoyoshi F, Sakamoto W. Functional divergence of the TFL1-like gene family in Arabidopsis revealed by characterization of a novel homologue. Genes Cells, 2001, 6: 327–336

[9]Yoo S Y, Kardailsky I, Lee J S, Weigel D, Ahn J H. Acceleration of ?owering by overexpression of MFT (MOTHER OF FT AND TFL1). Mol Cells, 2004, 17: 95–101

[10]Yamaguchi A, Kobayashi Y, Goto K, Abe M, Araki T. TWIN SISTER OF FT (TSF) acts as a floral pathway integrator redundantly with FT. Plant Cell Physiol, 2005, 46: 1175–1189

[11]Corbesier L, Vincent C, Jang S, Fornara F, Fan Q, Searle I, Glakountis A, Farrona S, Gissot L, Turnbul C, Coupland G. FT protein movement contributes to long-distance signaling in floral induction of Arabidopsis. Science, 2007, 316: 1030–1033

[12]Jaeger K E, Wigge P A. FT protein acts as a long-range signal in Arabidopsis. Curr Biol, 2007, 17: 1050–1054

[13]Zeevaart J A D. Leaf-produced ?oral signals. Curr Opin Plant Biol, 2008, 11: 541–547

[14]Tamaki S, Matsuo S, Wong H, Yokoi S, Shimamoto K. Hd3a protein is a mobile flowering signal in rice. Science, 2007, 316:1033–1036

[15]Taoka K, Ohki I, Tsuji H, Furuita K, Hayashi K, Yanase T, Yamaguchi M, Nakashima C, Purwestri Y A, Tamaki S, Ogaki Y, Shimada C, Nakagawa A, Kojima C, Shimamoto K. 14-3-3 proteins act as intracellular receptors for rice Hd3a florigen. Nature, 2011, 476: 332–335

[16]Navarro C, Abelenda J A, Cruz-Oró E, Cuéllar C A, Tamaki S, Silva J, Shimamoto K, Prat S. Control of flowering and storage organ formation in potato by FLOWERING LOCUS T. Nature, 2011, 478: 119–121

[17]Ruonala R, Rinne P L H, Kangasjärvi J, van der Schoot C. CENL1 expression in the rib meristem affects stem elongation and the transition to dormancy in Populus. Plant Cell, 2008, 20: 59–74

[18]Böhlenius H, Huang T, Charbonnel-Campaa L, Brunner A M, Jansson S, Strauss S H, Nilsson O. CO/FT regulatory module controls timing of ?owering and seasonal growth cessation in trees. Science, 2006, 312: 1040–1043

[19]Lifschitz E, Eviatar T, Rozman A, Shalit A, Goldshmidt A, Amsellem Z, Alvarez J P, Eshed Y. The tomato FT ortholog triggers systemic signals that regulate growth and ?owering and substitute for diverse environmental stimuli. Proc Natl Acad Sci USA, 2006, 103: 6398–6403

[20]Hedman H, Källman T, Lagercrantz U. Early evolution of the MFT-like gene family in plants. Plant Mol Biol, 2009, 70: 359–369

[21]Gubler F, Millar A A, Jacobsen J V. Dormancy release, ABA and pre-harvest sprouting. Curr Opin Plant Biol, 2005, 8: 183–187

[22]Xi W Y, Liu C, Hou X L, Yu H. MOTHER OF FT AND TFL1 regulates seed germination through a negative feedback loop modulating ABA signaling in Arabidopsis. Plant Cell, 2010, 22: 1733–1748

[23]Nakamura S, Abe F, Kawahigashi H, Nakazono K, Tagiri A, Matsumoto T, Utsugi S, Ogawa T, Handa H, Ishida H, Mori M, Kawaura K, Ogihara Y, Miura H. A wheat homolog of MOTHER OF FT AND TFL1 acts in the regulation of germination. Plant Cell, 2010, 23: 3215–3229

[24]Argiriou A, Michailidis G, Tsaftaris A S. Characterization and expression analysis of TERMINAL FLOWER1 homologs from cultivated alloteraploid cotton (Gossypium hirsutum) and its diploid progenitors. J Plant Physiol, 2008, 165: 1636–1646

[25]Dong R(东锐), Yuan H-Y(院海英), Gu C(顾超), Zheng Y-Y(郑银英), Huang X-Z(黄先忠), Cui B-M(崔百明). Clone and primary analysis of the function of GhFTL1 gene in cotton (Gossypium hirsutum). Cotton Sci (棉花学报), 2011, 23(6): 515–521 (in Chinese with English abstract)

[26]Hu G-H(胡根海), Yu S-X(喻树迅). Extraction of high-quality total RNA in cotton leaf with improved CTAB method. Cotton Sci (棉花学报), 2007, 19(1): 69–70 (in Chinese with English abstract)

[27]Kumar S, Nei M, Dudley. MEGA: a Biologist-centric software for evolutionary of DNA and protein sequences. Briefings Bioninform, 2008, 9: 299–306

[28]Reiersen H, Rees A R. The hunchback and its neighbours: proline as an environmental modulator. Trends Biochem Sci, 2001, 26: 679–684

[29]Chardon F, Damerval C. Phylogenomic analysis of the PEBP gene family in cereals. J Mol Evol, 2005, 61: 579–590

[30]Carmel-Goren L, Liu Y S, Lifschitz E, Zamir D. The SELF-PRUNING gene family in tomato. Plant Mol Biol, 2003, 52: 1215–1222

[31]Carmona M J, Calonje M, Martínez-Zapater J M. The FT/TFL1 gene family in grapevine. Plant Mol Biol, 2007, 63: 637–650

[32]Igasaki T, Watanabe Y, Nishiguchi M, Kotoda N. The FLOWER LOCUS T/TERMINAL FLOWER 1 family in Lombardy poplar. Plant Cell Physiol, 2008, 49: 291–300

[33]Danilevskaya O N, Meng X, Hou Z, Ananiev E V, Simmons C R. A genomic and expression compendium of the expanded PEBP gene family from maize. Plant Physiol, 2008, 146: 250–264

[34]Sun H-B(孙洪波), Jia Z(贾贞), Han T-F(韩天富). Roles of PEBP family genes in the development of plants. Plant Physiol J (植物生理学通讯), 2009, 45(8): 739–747 (in Chinese with English abstract)

[35]Karlgren A, Gyllenstrand N, Kallman T, Sundstrom J F, Moore D, Lascoux M, Lagercrantz U. Evolution of the PEBP gene family in plants: functional diversi?cation in seed plant evolution. Plant Physiol, 2011, 156: 1967–1977

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