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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (08): 1391-1399.doi: 10.3724/SP.J.1006.2013.01391

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Expression Analysis of GbMFT1 Gene in Gossypium barbadense L.

GU Chao1,LI Chao1,LI Xiao-Bo2,XIAO Xiang-Wen2,CUI Bai-Ming1,HUANG Xian-Zhong1,*   

  1. 1 Key Laboratory of Agrobiotechnology, College of Life Sciences, Shihezi University, Shihezi 832003, China; 2 Key Laboratory of Chemistry of Plant Resources in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China
  • Received:2013-01-29 Revised:2013-04-22 Online:2013-08-12 Published:2013-05-20
  • Contact: 黄先忠, E-mail: xianzhongh106@163.com, Tel: 0993-2057262

Abstract:

One MFT (MOTHER OF FT AND TFL1)-like homolog was isolated from Gossypium barbadense L. cv. Xinhai14 through RT-PCR and RACE method, which was designated as GbMFT1 gene. The open reading frame (ORF) of GbMFT1 is 528 bp in length, encoding a putative protein of 175 amino acids. GbMFT1 gene has a phosphatidylethanolamin-binding protein (PEBP) conserved motif. Liking almost all MFT-like proteins, GbMFT1 protein has a conserved proline residue near the C-terminus. Phylogenetic analysis revealed that GbMFT1 showed closer kinship with VvMFT and SP2G, indicating that they belong to the same evolutionary branch. Expression analysis by qRT-PCR indicated that GbMFT1 displayed a much broader expression range, with a maximum expression in petal. GbMFT1 was also expressed in different developmental stages of cotton fibres, especially in the two DPA ovules and nine DPA fibres. Expression analysis by semi-quantitative method indicated that GbMFT1 had a high expression level at the begining of seed germination. There existed no significant difference of expression in germinating seeds treated with different concentrations of ABA, indicating the expression of GbMFT1 is not regulated by ABA. Our results lay a good foundation in studying the function of cotton GbMFT1 gene further.

Key words: GbMFT1 gene, Early flowering, Gene expression, Florigen

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