Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (10): 1746-1753.doi: 10.3724/SP.J.1006.2013.01746

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Dissection of Elite Line Zhongpin 03-5373 Pedigree and Identification of Candidate Markers Related to Resistance to Soybean Cyst Nematode

ZHANG Shan-Shan1,**,LI Ying-Hui1,**,LI Jin-Ying2,QIU Li-Juan1,*   

  1. 1 National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Germplasm Utilization, Agriculture of Ministry/ Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 Institute of Industrial Crops, Zhengzhou/ National Subcenter for Soybean Improvement, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
  • Received:2013-01-28 Revised:2013-05-11 Online:2013-10-12 Published:2013-08-01
  • Contact: 邱丽娟, E-mail: qiu_lijuan@263.net, Tel: 010-82105843

Abstract:

Zhongpin 03-5373 with resistance to SCN3 is an elite soybean line, which is traced back to 10 parental lines. Three elite resistant sources (Huipizhiheidou, Peking and PI437654), diversely used in the soybean cyst nematode resistant breeding, were included in the pedigree of Zhongpin 03-5373. In this study, 152 molecular markers were selected to detect polymorphism among the 11 materials. A total of 437 alleles were identified with average of 2.9, ranging from two to five alleles on each locus. The average of Nei’s genetic identity among pairwise cultivars were 0.458, the haplotypes formed by four SSR markers (Satt152, Satt179, Barcsoyssr_18_107, and Satt196) could distinguish 11 cultivars in this study. Pedigree tracing elucidated genomic variation in breeding. IBD analysis showed that Huipizhiheidou contributed the most of specific alleles (39) to Zhongpin 03-5373 among three resistant genetic resources (Huipizhiheidou, Peking, and PI437654), followed by PI437654 (6). In addition, 20 markers identified were related to the resistance to SCN 3. These results supplied the information for further cloning resistant gene and resistant breeding by marker assistant selection.

Key words: Soybean, Soybean cyst nematode, Pedigree analysis, Molecular marker

[1]Lu W-G(卢为国), Gai J-Y(盖钧镒), Li W-D(李卫东). Sampling survey and identification of races of soybean cyst nematode (Heterodera glycines Ichinohe) in Huang-Huai Valleys. Sci Agric Sin (中国农业科学), 2006, 39(2): 306–312 (in Chinese with English abstract)



[2]Liu H-Q(刘汉起), Shang S-G(商绍刚), Huo H(霍虹), Wu H-L(吴和礼). Resistance of soybean varieties to race 1, 3 and 4 of soybean cyst nematode. Soybean Sci (大豆科学), 1989, 8(1): 113–114 (in Chinese)



[3]Liu W-Z(刘维志), Liu Y(刘晔), Chen P-S(陈品三). Preliminary report of identify the races of SCN in some city & county of Northeast. J Shenyang Agric Coll (沈阳农学院学报), 1984, (2): 74–78 (in Chinese)



[4]Zhang W-B(张闻博), Jiang H-W(蒋洪蔚), Li C-D(李灿东), Qiu P-C(邱鹏程), Qi Z-M(齐照明), Liu C-Y(刘春燕), Jiang W(姜威), Wang J(王晶), Hu G-H(胡国华), Chen Q-S(陈庆山). Integration of QTLs related to soybean cyst nematode resistance based on meta-analysis. Chin J Oil Crop Sci (中国油料作物学报), 2010, 32(1): 104–106 (in Chinese with English abstract)



[5]Liu G(刘刚), Lu S-X(鲁绍雄). Strategies for fine mapping of QTL with linkage disequilibrium. J Domestic Anim Ecol (家畜生态学报), 2006, 27(6): 187–201 (in Chinese)



[6]Russell J R, Ellis R P, Thomas W T B, Waugh R, Provan J, Booth A, Fuller J, Lawrence P, Yoang G, Powell W. A retrospective analysis of spring barley germplasm development from 'foundation genotypes' to currently successful cultivars. Mol Breed, 2000, 6: 553–568



[7]Guan R-X(关荣霞), Qin J(秦君), Hu J-S(胡静深), Chen W-X(陈为秀), Chang R-Z(常汝镇), Liu Z-X(刘章雄), Qiu L-J(邱丽娟). Genetic composition of elite soybean cultivar Hefeng 25. Acta Agron Sin (作物学报), 2009, 35(9): 1590–1596 (in Chinese with English abstract)



[8]Qin J, Yang R Q, Jiang C X, Li W B, Li Y H, Guan R X, Chang R Z, Qiu L J. Discovery and transmission of functional QTL in the pedigree of an elite soybean cultivar Suinong14. Plant Breed, 2010, 129: 235–242



[9]Liu Z-X(刘章雄), Lu W-G(卢为国), Chang R-Z(常汝镇), Qiu L-J(邱丽娟). Creation of new soybean SCN4-resistant lines. Soybean Sci (大豆科学), 2008, 17(6): 911–914 (in Chinese with English abstract)



[10]Liu P-Y(刘佩印). Advances in study of screening and utilization for antigen to soybean cyst nematode. Heilongjiang Agric Sci (黑龙江农业科学), 2005, (6): 44–47 (in Chinese with English abstract)



[11]Zhang G-D(张国栋). Advances in study of races of soybean cyst nematode and resistance of cultivars of soybean in America. Soybean Sci (大豆科学), 1994, 13(3): 252–260 (in Chinese)



[12]Anand S C. Registration of ‘Hartwig’ soybean. Crop Sci, 1992, 32: 1069–1070



[13]Li Y(李莹), Wang Z(王志), Li Y-P(李原萍). Research on germplasm resources of soybean in Shanxi Province. J Shanxi Agric Sci (山西农业科学), 2012, 40(7): 791–795 (in Chinese with English abstract)



[14]Liu Y-L(刘玉林). Constructing Genetic Linkage Map and Mapping QTLs for Agronomic Traits in Soybean. MS Thesis of Chinese Academy of Agricultural Sciences, 2011. pp 42–43 (in Chinese with English abstract)



[15]Li Y H, Marinus J M S, Chang R Z, Qiu L J. Genetic diversity and association mapping in a collection of selected Chinese soybean accessions based on SSR marker. Conserv Genet, 2011, 12, 1145–1157



[16]Concibido V C, Diers B W, Arelli P R. A decade of QTL mapping for cyst nematode resistance in soybean. Crop Sci, 2004, 44: 1121–1131



[17]Song Q J, Marek L F, Shoemaker R C, Lark K G, Concibido V C, Delannay X, Specht J E, Cregen P B. A new integrated genetic linkage map of the soybean. Theor App Genet, 2004, 109: 122–128



[18]Liu Y L, Li Y H, Zhou G A, Uzokwe N, Chang R Z, Chen S Y, Qiu L J. Development of soybean EST-SSR markers and their use to assess genetic diversity in the Subgenus Soja. Agric Sc: China, 2010, 9(10): 1423–1429



[19]Nei M, Li W H. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA, 1979, 76: 5269–5273



[20]Zhai H-Q(翟虎渠), Wang J-K(王建康). Application of Quantitative Genetics (应用数量遗传学). Beijing: China Agriculture Science and Technology Press, 2007. pp 42–43 (in Chinese)



[21]Fernández M E, Figueiras A M, Benito C. The use of ISSR and RAPD markers for detecting DNA polymorphism genotype identification and genetic diversity among barley cultivars with known origin. Theor Appl Genet, 2002, 104: 845–851



[22]Smith J S C, Chin E C L, Shu H, Smith O S, Wall S J, Senior M L, Mitchell S E, Kresovich S, Ziegle J. An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L) comparisons with data from RFLPs and pedigree. Theor App Genet, 1997, 95: 163–173



[23]Evans K M, Patocchi A, Rezzonico F, Mathis F, Durel C E, Fernández-Fernández F, Boudichevskaia A, Dunemann F, Stankiewicz-Kosyl M, Gianfranceschi L, Komjanc M, Lateur M, Madduri M, Noordijk Y, van de Weg W E. Genotyping of pedigreed apple breeding material with a genome-covering set of SSRs: trueness-to-type of cultivars and their parentages. Mol Breed, 2011, 28: 535–547



[24]William J H, David L H, Wayne W X, Daniel J G, Thomas J A, Todd R, Jeffrey A J, Jia G F, Nathan M S, Carroll P V, Robert M S. The composition and origins of genomic variation among individuals of the soybean reference cultivar Williams 82. Plant Physiol, 2011, 155: 645–655



[25]Yuan C-P(袁翠平), Shen B(沈波), Dong Y-S(董英山). Released soybean varieties resistant to cyst nematode in China and their resistance genetic derivation. Soybean Sci (大豆科学), 2009, 28(6): 1049–1053 (in Chinese with English abstract)



[26]Lu W-G(卢为国), Li W-D(李卫东), Liang H-Z(梁惠珍), Wang S-F(王树峰), Sun S(孙慎). Progress of soybean cyst nematode gene mapping. J Henan Agricl Sci (河南农业科学), 2005, (11): 13–17 (in Chinese)



[27]Cregan P B, Mudge J, E W Fickus. Two simple sequence repeat markers to select for soybean cyst nematode resistance conditioned by the rhg1 locus. Theor App Gene, 1999, 99: 811–818



[28]Cook D E, Lee T G, Guo X L, Melito S, Wang K, Bayless A, Wang J P, Hughes T J, Willis D K, Clemente T, Diers B W, Jiang J M, Hudson M E, Bent A F. Copy number variation of multiple genes at Rhg1 mediates nematode resistance in Soybean. Science, 2012, 338: 1206–1209



[29]Chang W(常玮), Hang Y-P(韩英鹏), Hu H-B(胡海波), Li W-B(李文滨). Mining candidate genes for resistance to soybean cyst nematode based on meta-analysis and domains annotations. Sci Agric Sin (中国农业科学), 2010, 43(23): 4787–4795 (in Chinese with English abstract)
[1] Zhai Sheng-Nan, Cao Xin-You, Li Hao-Sheng, Li Ji-Hu, Li Fa-Ji, Liu Jin-Dong, Xia Xian-Chun, Lyu Ying-Ying, Ma Rui-Feng, Wang Ying, Geng Hong-Wei, Liu Jian-Jun. Analysis of the genetic effects of allelic variation at the Pod-A1, Pod-D1, and Pod-2D loci on peroxidase activity in wheat grains [J]. Acta Agronomica Sinica, 2026, 52(6): 1593-1603.
[2] Peng Jia-Luo, Li Ying, Li Dan-Dan, Yang Jun-Ning, Guo Xue-Feng, Zhang Wen-Jiao, Yu Xiao-Xue, Zhou Ya-Rong, Wang Zhen-Yu, Wang Cai-Xiang, Ma Xiong-Feng, Su Jun-Ji. Identification of class I LBD family members in upland cotton and function and haplotype analyses of GhLBD6 in regulating flowering period [J]. Acta Agronomica Sinica, 2026, 52(6): 1682-1697.
[3] Tang Kuan-Qiang, Li Gong-Yun, Song Mei-Yi, Zhao Xue, Chang Chun-Ling. Genome-wide association analysis and prediction model construction for soybean plant height [J]. Acta Agronomica Sinica, 2026, 52(6): 1743-1756.
[4] Zhang Xian-Feng, Guo Li-Jian, Li Kang-Chun, Kong Bin-Xue, Liu Yu-Fang, Che Zhuo, Yang De-Long. Identification of the ABHD6 gene family and development of functional markers for grain weight in wheat [J]. Acta Agronomica Sinica, 2026, 52(6): 1711-1727.
[5] Yao Shu, Guo Kai-Yue, Zhai Hui-Hui, Yao Jia-Hui, Deng Wen-Qi, Yan Ling, Huang Chi, Gao Yang, Yu Yan-Ran, Zhao Zhen-Bang, Li Ying-Hui, Wang Xiao-Bo, Li Jia-Jia. Comprehensive evaluation of low-iron tolerance and screening of elite germplasm at the soybean seedling stage [J]. Acta Agronomica Sinica, 2026, 52(5): 1373-1387.
[6] Wang Chu-Rui, Li Kai-Xiang, Zhao Zhi, Xiao Lu, Tang Guo-Yong, Zhao Zhi-Gang, Xu Liang, Du De-Zhi, Liu Hai-Dong. Development and application of KASP markers for functional sites of the early flowering gene BnCRY2 in Brassica napus L. spring rapeseed [J]. Acta Agronomica Sinica, 2026, 52(3): 708-721.
[7] Zhang Qing, Yang Yu, Guo Qian, Yue Pei-Yao, Yin Cong-Cong, Niu Jing-Ping, Zhao Jin-Zhong, Du Wei-Jun, Yue Ai-Qin. Cloning and functional analysis of the soybean GmARA6a gene in response to salt stress [J]. Acta Agronomica Sinica, 2026, 52(2): 480-493.
[8] Wang Yue-Sheng, Ge Dong-Dong, Cheng Lan-Fei, Chen Chun-Huan, Wang Chang-You, Liu Xin-Lun, Li Ting-Dong, Deng Ping-Chuan, Ji Wan-Quan, Zhao Ji-Xin. Molecular cytogenetic and disease resistance characterization of the wheat- Psathyrostachys huashanica disomic substitution line 16DH25-7 [J]. Acta Agronomica Sinica, 2026, 52(2): 433-445.
[9] WANG Ke-Jing, LI Xiang-Hua. Endangerment assessment of the perennial species G. tabacina and G. tomentella of the genus Glycine Willd. in China [J]. Acta Agronomica Sinica, 2025, 51(8): 2009-2019.
[10] MENG Ran, LI Zhao-Jia, FENG Wei, CHEN Yue, LIU Lu-Ping, YANG Chun-Yan, LU Xue-Lin, WANG Xiu-Ping. Comprehensive evaluation of salt tolerance at different growth stages of soybean and screening of salt-tolerant germplasm [J]. Acta Agronomica Sinica, 2025, 51(8): 1991-2008.
[11] HE Hong-Li, ZHANG Yu-Han, YANG Jing, CHENG Yun-Qing, ZHAO Yang, LI Xing-Nuo, SI Hong-Liang, ZHANG Xing-Zheng, YANG Xiang-Dong. Creation and physiological analysis of an e1-as gene mutant in soybean [J]. Acta Agronomica Sinica, 2025, 51(8): 2228-2239.
[12] HU Meng, SHA Dan, ZHANG Sheng-Rui, GU Yong-Zhe, ZHANG Shi-Bi, LI Jing, SUN Jun-Ming, QIU Li-Juan, LI Bin. QTL mapping and candidate gene screening for branch number in soybean [J]. Acta Agronomica Sinica, 2025, 51(7): 1747-1756.
[13] WANG Qiong, ZOU Dan-Xia, CHEN Xing-Yun, ZHANG Wei, ZHANG Hong-Mei, LIU Xiao-Qing, JIA Qian-Ru, WEI Li-Bin, CUI Xiao-Yan, CHEN Xin, WANG Xue-Jun, CHEN Hua-Tao. Genome-wide association analysis and candidate genes prediction of flowering time and maturity date traits in soybean (Glycine max L.) [J]. Acta Agronomica Sinica, 2025, 51(6): 1558-1568.
[14] YIN Cong-Cong, LI Rui-Qi, YUE Pei-Yao, LI Chen, NIU Jing-Ping, ZHAO Jin-Zhong, DU Wei-Jun, YUE Ai-Qin. Establishment and application of a visual detection method for soybean mosaic virus SC15 based on closed dumbbell mediated isothermal amplification [J]. Acta Agronomica Sinica, 2025, 51(5): 1248-1260.
[15] ZHAN Zong-Bing, JIN Qi-Feng, LIU Di, LYU Ying-Chun, GUO Ying, ZHANG Xue-Ting, HU Meng-Xia, WANG Shang, YANG Fang-Ping. Molecular characterization and evaluation of important traits of landrace wheat Laomangmai in Gansu province, China [J]. Acta Agronomica Sinica, 2025, 51(3): 609-620.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!