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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (05): 769-778.doi: 10.3724/SP.J.1006.2014.00769

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Physiological Characterization and Gene Fine Mapping of a Leaf Early Senescence and Salt-Sensitive Mutant osles in Rice

MAO Jie-Jing1,ZHAO Chen-Chen1,HUANG Fu-Deng2,PAN Gang1,*,CHENG Fang-Min1,*   

  1. 1 College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China; 2 Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China?
  • Received:2013-10-14 Revised:2014-01-12 Online:2014-05-12 Published:2014-03-24

Abstract:

A osles (Oryza sativa leaf early-senescence and salt-sensitive) mutant, produced by 60Co γ-radiation treatment of indica cultivar Zixuan 1, was identified. The osles showed yellow at tip and margin of leaf blade with red brown spots during growth at tillering stage. In addition, under salt stress, the leaves were rolled and wilted, and plant height and plant dry weight were significantly decreased. Compared with the control plant, in the mutant plant, the contents of chlorophyll and soluble protein decreased significantly, while the activities of SOD and POD increased significantly; higher soluble protein content appeared in the 1st and 2nd leaves from top, and decreased in the 3rd leaf. Genetic analysis indicated that osles was controlled by a recessive nuclear gene, which was finely mapped in a 210 kb interval between two markers IN6-005769-11/12 and RM20547 on long arm of chromosome 6. These results will facilitate the positional cloning and functional studies of the gene.

Key words: Rice, osles, Gene mapping, Physiological analysis

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