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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (05): 806-812.doi: 10.3724/SP.J.1006.2015.00806

• RESEARCH NOTES • Previous Articles     Next Articles

Identification of Resistance to Brown Rust and Molecular Detection of Bru1 Gene in 31 Wild Core Sugarcane Germplasms

LI Wen-Feng,WANG Xiao-Yan,HUANG Ying-Kun*,ZHANG Rong-Yue,SHAN Hong-Li,YIN Jiong,dLUO Zhi-Ming   

  1. Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences / Yunnan Province Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan 616699, China
  • Received:2014-10-31 Revised:2015-02-06 Online:2015-05-12 Published:2015-03-03
  • Contact: 黄应昆,E-mail: huangyk64@163.com,Tel: 0873-7227017

Abstract:

The objectives of this study was to clarify the level of resistance to Puccinia melanocephala and the distribution of Bru1 in sugarcane wild germplasm collections. Resistance of 31 wild core germplasm from National Nursery of Sugarcane Germplasm Resources (NNSGR, Kaiyuan, China) was identified at seedling stage, and the resistance gene Bru1 was detected in 2013. The results showed that 28 out of 31 materials (90.3%) were highly resistant (Grade 1) or moderately resistant (Grade 3). Of them, nineteen (61.3%) were highly resistant (Grade 1), three (9.7%) were resistant (Grade 2) and six (19.4%) were moderately resistant. Bru1 was detected in eight (25.8%) resistant materials (Guizhou 78-2-12, Yunnan 97-4, E. rockii 95-19, E. rockii 95-20, Yunnan 83-224, Guangxi 79-8, Yunnan 95-35, and Guangxi 89-13). The absence of Bru1 in other 20 resistant and three susceptible entries suggests they may contain brown rust resistance-associated genes other than Bru1. Therefore the elite brown rust resistance gene should be contained in numerous sugarcane wild core germplasm collections of NNSGR, and be valuable for future brown rust resistant breeding programs in sugarcane.

Key words: Sugarcane, Wild core germplasm, Brown rust resistance gene Bru1, Molecular detection.

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