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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (04): 600-608.doi: 10.3724/SP.J.1006.2016.00600

• RESEARCH NOTES • Previous Articles     Next Articles

Photosynthetic Characteristics of Transgenic Wheat Expressing Maize C4-Type NADP-ME Gene

WANG Yong-Xia1,2,DU Xin-Hua1,2,XU Wei-Gang1,2,*,QI Xue-Li2,LI Yan2,WANG Hui-Wei2,HU Lin2   

  1. 1 Nanjing Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; 2 Henan Provincial Laboratory of Wheat Biology / Wheat Research Institute , Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
  • Received:2015-10-12 Revised:2016-01-11 Online:2016-04-12 Published:2016-01-25
  • Contact: 许为钢, E-mail: xuwg1958@163.com, Tel: 0371-65712307 E-mail:wangyongxia005@163.com
  • Supported by:

    This study was supported by the National GMO Program of China (2011ZX08002-003) and the China Agriculture Research System (CARS-03-03B).

Abstract:

To explore the physiological characteristics of the transgenic wheat expressing maize C4-type NADP-ME, we introduced NADP-ME into the C3 crop wheat by using particle bombardment transformation. Two transgenic wheat lines (10T(9)-1-1, 10T(9)-225-4) and parental control (Zhoumai 19)were used to study molecular characteristics and photosynthesis property, to reveal the mechanism. The results showed that the NADP-ME sequence was integrated into wheat genome, and the transcription and translation were exactly same as expect. The enzyme activity of NADP-ME in flag leaf in transgenic plants were increased significantly than untransformed plants, for instance it was increased 1.33 and 1.13 times on the 7th day after flowering. Net photosynthetic rate (Pn) of flag leaf in transgenic plants obviously decreased when compared to the untransformed plants. On the 7th day after anthesis, Pn of transgenic wheat decreased by 17.26% and 10.35%. The yield and 1000-grain weight were decreased than the control. Utilization efficiency on strong light utilizing and ability of CO2 assimilation in transgenic line 10T(9)-225-4 were significantly declined, photosynthesis rate was also decreased. stomatal opening rate and stomatal conductance were significant decrease, malic acid content of transgenic wheat reducing 5.6% while pyruvate level is raised by 17.1%, and Pn of transgenic wheat can be restored by feeding with exogenous malate. Those results indicated that the transgenic wheat expressing maize NADP-ME gene showed lower photosynthetic characteristics than the control, the reason was maybe the decrease of stomatal aperture caused by decline of malic acid content.

Key words: Transgenic wheat, NADP-dependent malic enzyme, Net photosynthetic rate, Stomata conductance

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