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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (12): 1735-1742.doi: 10.3724/SP.J.1006.2016.01735

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS •     Next Articles

Fine Mapping of Polycyetic Gene (Bjmc2) in Brassica juncea L.

WANG Gang,ZHANG Xiang-Xiang,XU Ping,LYU Ze-Wen,WEN Jing,YI Bin,MA Chao-Zhi,TU Jin-Xing, FU Ting-Dong,SHEN Jin-Xiong*   

  1. National Key Laboratory of Crop Genetic Improvement / National Research Center of Rapeseed Engineering and Technology, Huazhong Agricultural University, Wuhan 430070, China
  • Received:2016-04-01 Revised:2016-06-20 Online:2016-12-12 Published:2016-07-04
  • Contact: Shen Jinxiong, E-mail: jxshen@mail.hzau.edu.cn, Tel: 13667227126 E-mail:wanggang2014@webmail.hzau.edu.cn
  • Supported by:

    This study was supported by the National Science Foundation of China (31571698).

Abstract:

Multilocular plants have a higher seed yield than bilocular plants in B. juncea, mapping and cloning the multilocular gene(s) might be helpful for rapeseed genetic improvement and explanation of the multilocular trait development mechanism. This study verified backcross populations derived from JD11-2 family different at BjMc2 locus only can be used for BjMc2 mapping. Using AFLP assay and BSA method in BC5 and BC6 generations, one AFLP marker linked to the target gene was obtained and converted to SCAR marker (SC1). On the basic of homologous sequences of the AFLP maker in B. rapa, 11 SSR markers and one SCAR marker were identified. Through the screening in theZBjH BAC library of Brassica juncea, two BACs covered the target area were selected and one SSR marker was developed. All the developed SCAR and SSR markers were then used to detect the BC7 population, and a linkage map for the bilocular gene BjMc2 was built. ZX17 and BACsr96, the closest flanking markers, were mapped at 0.048 cM and 0.340 cM distant from the BjMc2 gene, respectively. Bjmc2 is positioned of 68 kb between 946 kb and 1014 kb in the scaffold000019 physical map of A7 in B. rapa. This result would lay a foundation for cloning polycyetic gene Bjmc2 and selectingpolycyetic lines by marker-assisted selection.

Key words: Brassica juncea, Polycyetic silique, BjMc2, Molecular marker

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