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Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (12): 1754-1763.doi: 10.3724/SP.J.1006.2016.01754

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Phenotypic Characterization and Gene Mapping of a Thermo-sensitive AlbinoLeaf Mutant tsa1 in Rice

LIU Yu-Long1,**,LIU Feng1,**,ZHOU Kun-Neng3,SU Xiao-Mei1,FANG Xian-Wen2,ZHANG Yun-Hui2,*,BAO Yi-Qun1,*   

  1. 1 College of Life Science, Nanjing Agricultural University, Nanjing 210095, China; 2 Institute of Food Crops, Jiangsu Academy of Agricultural Sciences / Jiangsu Agricultural Germplasm Resources Conservation and Utilization Platform, Nanjing 210014, China; 3 Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China
  • Received:2016-03-21 Revised:2016-07-11 Online:2016-12-12 Published:2016-08-24
  • Contact: Bao Yiqun,E-mail:baoyiqun@njau.edu.cn,Tel:025-84396795;Zhang Yunhui,E-mail:zyhrice@163.com,Tel:025-84390321 E-mail:2013116120@njau.edu.cn
  • Supported by:
    This study was supported by the National Natural Science Foundation (31401036), the Natural Science Foundation of Jiangsu Province (BK20130706), the Jiangsu Province Agricultural Science and Technology Independent Innovation Foundation [CX(14)5005], the Jiangsu Academy of Agricultural Sciences Fundamental Research Projects [ZX(15)4015], and the Natural Science Foundation of Anhui Province (1408085MKL62).

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Abstract:

A thermo-sensitive albino leaf mutant tsa1 was obtained from Ningjing36 induced by Ethyl methane sulfonate (EMS). The mutant developed albinotic leaves under low temperature(20-24°C), and the color of mutant leaves was comparable with the wild-type under high temperature (28-32°C). Compared with wild type, the chlorophyll a and carotene contents of mutant albinotic leaves obviously decreased under low temperature condition. With microscopeobservation, the quantity of normal chloroplast significantly decreased, besides, there were a large number of small abnormal chloroplasts in albinotic leaves. The abnormal chloroplast in albinotic tissue of mutant had no obvious thylakoid stack under transmission electron microscopy. These results indicate that tsa1 mutant has defect on chloroplast development under low temperature. Genetic analysis indicated that the mutant phenotype was controlled by a single recessive nuclear gene. Genetic mapping of the mutant gene was conducted using 368 recessive individuals from F2 population of tsa1/Nanjing 11, the target gene was finally located in a 163 kb region on the chromosome 5. This study provides a basis for map-based cloning and functional analysis of the target gene.

Key words: Rice(OryzasativaL.), Thermo-sensitive, Albino, Mutant, Chloroplastdevelopment

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