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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (09): 1308-1318.doi: 10.3724/SP.J.1006.2017.01308

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Effects of Different Dehydrating Agents on Seed Quality and Gene Expression inHybrid Rice Seed Production

SHEN Hang-Qi1,HU Wei-Min1,LIN Cheng1,GUAN Ya-Jing1,LIU Hong-You2,AN Jian-Yu1,HU Jin1,*   

  1. 1 Seed Science Center, Institute of Crops Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China; 2 Seed Management Station of Pujiang County, Jinhua 322200, China
  • Received:2016-12-09 Revised:2017-05-10 Online:2017-09-12 Published:2017-05-25
  • Contact: Hu JIn, E-mail: jhu@zju.edu.cn E-mail:736935396@qq.com
  • Supported by:

    This study was supported by the China Special Fund for Agro-Scientific Research in the Public Interest (201203052), the Project of Science and Technology Department of Zhejiang Province (2013C02005, 2013C32023), the Natural Sciences Fund of Zhejiang Province (LZ14C130002, LY15C130002), and Jiangsu Collaborative Innovation Center for Modern Crop Production.

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Abstract:

In order to accelerate seed dehydration process in hybrid rice seed production, the effects of different dehydrating agents on seed dehydration and quality of cv. Y Liangyou 689 were studied. Different dehydrating agents were sprayed on rice spike at late dough stage inseed production in the field. All the eleven combination treatments could accelerate seed dehydration. Among the treatments, No.7 and No.9 dehydrating agents significantly decreased seed moisture content by 4.6% and 3.6%, respectively as compared with the control, and had no negative effects on 1000-grain weight, seed germination and seedling growth. Moreover, these two agents significantly increased the contents of soluble protein, soluble sugar, ABA and GA3 in seeds. After storage at room temperature for sixmonths, No.7, No.8, and No.9 agents had no negative effects on seed germination and seedling growth as compared with the control. And there were no significant differences in soluble protein content, α-amylase activity and ABA content among No.7, No.9 agents and the control. In addition, No.7 agent treatment had better effect in decreasing seed moisture content than No.9 agent, suggestingthat No.7 agent was a suitable seed dehydrating agent inhybrid rice (cv. Y Liangyou 689) seed production. Further studies showed that No.7, No.8, and No.9 agents could increase the expression of OsNECD1, OsNCED2 and reduce the expression of OsGA2ox1 in the seeds. As compared with the seeds without storage, after the storage for six month the expression of OsNECD1 and OsNCED2 decreased, however the expression of OsCYP707A5 and OsGA2ox1 increased in seeds treated by the three kinds of dehydrating agents. The results showed the effect of dehydrating agents on ABA biosynthesis genes and GA3 catabolism genes in seedswas weakenedby storage, and the high expression of OsCYP707A5 resulted in the decline of ABA content in seeds, which might be the main reason for increasing seed germination.

Key words: Hybrid rice, Dehydration agent, Seed quality, Mature dehydration, Gene expression

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