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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (12): 1791-1801.doi: 10.3724/SP.J.1006.2017.01791

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Correlation between Resistance to Fusarium Wilt and Expression of Flavonoid Metabolism Related Genes in Gossypium barbadense L.

HUANG Qi-Xiu,QU Yan-Ying,YAO Zheng-Pei,LI Meng-Yu,CHEN Quan-Jia*   

  1. College of Agronomy, Xinjiang Agriculture University / Key Laboratory of Agriculture Biological Technology, Xinjiang Agricultural University, Urumqi 830052
  • Received:2017-03-09 Revised:2017-07-23 Online:2017-12-12 Published:2017-08-10
  • Contact: 陈全家, E-mail: chqjia@126.com E-mail:13899525177@163.com
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31560409).

Abstract:

Fusarium wilt is one of the important factors that threaten the production of Gossypium barbadense. L. To expanding the molecular mechanism of resistance to Fusarium wilt will provide a solid foundation for cultivating resistant island cotton varieties and solve the problem of wilt disease. In this study DEG(Differentially Expressed Gene) was analyzed on the basis of sequencing of transgenic plants. The Disease resistance differentially expressed genes were analyzed at different inoculation time points of seven Gossypium barbadense. L varieties with different disease resistance levels, and the correlation between gene expression and disease index was analyzed. Flavonoid biosynthetic pathway genes were related to fusarium wilt resistance. qRT-PCR analysis showed that the expression of flavonoid metabolic pathway genes in resistant materials was significantly higher than that in susceptible materials. The expression levels of TT7, CHI and DFR, the key genes in flavonoid metabolic pathways, were significantly higher in resistant materials than in susceptible materials at multiple time points after inoculation, And the expression of CHI and DFR genes was negatively correlated with disease index. In summary, the flavonoid metabolic pathway-related genes have an effect on the resistance to fusarium wilt, among which CHI, TT7 and DFR genes are the key genes.

Key words: Gossypium barbadense, Fusarium wilt, Flavonoids, Expression analysis, RNA-seq

[1]孔庆平. 我国海岛棉生产概况及比较优势分析. 中国棉花, 2002, 12: 19–23
Kong Q P. Production situation and comparative advantage analysis of Gossypium barbadense in China. China Cotton, 2002, 12: 19–23 (in Chinese with English abstract)
[2]徐秋华, 张献龙, 聂以春, 冯纯大. 我国棉花抗枯萎病品种的遗传多样性分析. 中国农业科学, 2002, 35: 272–276
Xu Q H, Zhang X L, Nie Y C, Feng C D. Genetic diversity evaluation of cultivars (G. hirsumtum L.) resistant to fusarium wilt by RAPD markers. Sci Agric Sin, 2002, 35: 272–276 (in Chinese with English abstract)
[3]彭姜龙, 曲延英, 王莉萍, 王聪, 郝维维, 汪铖亮. 不同温度条件下接种棉花枯萎病菌海岛棉和陆地棉抗病性研究. 新疆农业科学, 2013, 50: 89–93
Peng J L, Qu Y Y, Wang L P, Wang C, Hao W W, Wang C L. A study on properties of resistance to Fusarium wilt in leaves of sea island cotton (Gossipium barbadense) and upland cotton (Gossipium hirsutum L.) under different temperature conditions. Xinjiang Agric Sci, 2013, 50: 89–93 (in Chinese with English abstract)
[4]校百才. 陆地棉抗枯、黄萎病性状配合力、遗传力的初步研究. 作物学报, 1985, 11: 267–273
Xiao B C. Preliminary study on cotton Fusarium and Verticillium wilt resistance traits, combining ability and heritability. Acta Agron Sin, 1985, 11: 267–273 (in Chinese with English abstract)
[5]Smith A L, Dick J B. Inheritance of resistance to Fusarium wilt in upland and sea island cottons as complicated by nematodes under field conditions. Phytopathology, 1960, 50: 44–48
[6]Yang J, Ma Q, Zhang Y, Wang X F, Zhang G Y, Ma Z Y. Molecular cloning and functional analysis of GbRVd, a gene in Gossypium barbadense that plays an important role in conferring resistance to Verticillium wilt. Gene, 2016, 575: 687–694
[7]Gao X Q, Wheeler T, Li Z H, Charles M K, He P, Shan L B. Silencing GhNDR1 and GhMKK2 compromises cotton resistance to Verticillium wilt. Plant J, 2 011, 66: 293–375
[8]Snyder M. RNA-Seq: a revolutionary tool for transcriptomics. Nat Rev Genet, 2009, 10: 57–63
[9]Xu L, Zhu L F, Tu L L, Liu L L, Yuan D J, Jin L, Long L, Zhang X L. Lignin metabolism has a central role in the resistance of cotton to the wilt fungus Verticillium dahliae as revealed by RNA-Seq-dependent transcriptional analysis and histochemistry. J Exp Bot, 2011, 62: 5607–5621
[10]王春晖. 陆地棉抗病自交系在黄萎病菌诱导下的转录组测序研究. 中国农业科学院硕士学位论文, 北京, 2013
Wang C H. Study on Transcriptome Sequencing of Cotton (Gossypium hirsutum L.) Resistant Inbred Line Induced by Verticillium. MS Thesis of Chinese Academy of Agricultural Sciences, Beijing, China, 2013 (in Chinese with English abstract)
[11]Hichri I, Barrieu F, Bogs J, Kappel C, Delrot S, Lauvergeat V. Recent advances in the transcriptional regulation of the flavonoid biosynthetic pathway. J Exp Bot, 2011, 62: 2465–2483
[12]Malhotra B, Onyilagha J C, Bohm B A, Towers G H N, James D, Harborne J B, French C J. Inhibition of tomato ringspot virus by flavonoids. Phytochemistry, 1996, 43: 1271–1276
[13]贾振华. 植物黄酮类化合物槲皮素与转录因子AtMYB44诱导和调控植物防卫反应的研究. 南京农业大学博士学位论文, 江苏南京, 2010
Jia Z H. Study on the Effects of Quercetin and Transcription Factor Atmyb44 on Plant Defense Response. PhD Dissertation of Nanjing Agricultural University, Nanjing, China, 2010 (in Chinese with English abstract)
[14]Anna K P, Edward F. S, Jaroslav P. Effect of flavonoids on mycelial growth of Verticillium albo-atrum. Biochem Syst & Ecol, 1995, 23: 683–693
[15]左涛, 赵树堂, 卢孟柱, 孙爱东, 王延伟, 贺伟. 杨树二氢黄酮醇-4-还原酶基因(DFR)的克隆及反义表达对儿茶素合成的影响. 东北林业大学学报, 2016, 10: 49–55
Zuo T, Zhao S T, Lu M Z, Sun A D, Wang Y W, He W. Cloning dihydroflavonol-4-reductase gene (DFR) of poplar and its antisense expression effects on catechin synthesis. J Northeast For Univ, 2016, 10: 49–55 (in Chinese with English abstract)
[16]马银平, 王付欣, 杨淳淋, 沈法富, 夏桂先. 海岛棉几丁质酶基因GbCHI的克隆与功能分析. 遗传, 2012, 34: 240–247
Ma Y P, Wang F X, Yang C L, Shen F F, Xia G X. Cloning and functional analysis of chitinase gene GbCHI from sea-island cotton (Gossypium barbadense). Hereditas (Beijing), 2012, 34: 240–247 (in Chinese with English abstract)
[17]Leslie A W, Li G Q, Doreen W, Imre E S, Keith R D. The phenylalanine ammonialyase gene family in Arabidopsis thaliana. Plant Mol Biol, 1995, 27: 327–338
[18]Wajad N, Abdul L T, Saghir A, Khalid M, Abid M, Zhou B L. Evaluation of Cotton Leaf Curl Virus Resistance in BC1, BC2, and BC3 Progenies from an Interspecific Cross between Gossypium arboreum and Gossypium hirsutum. PLoS One, 2014, 9: e111861
[19]Emilie F F, Bart P H J T. Physiology and molecular aspects of Verticillium wilt diseases caused by V. dahliae and V. albo-atrum. Mol Plant Pathol, 2006, 7: 71–86
[20]Zhang S, Klessig D F. MAPK cascades in plant defense signaling. Trends Plant Sci, 2001, 6: 520–527
[21]Kunkel B N, Brooks D M. Cross talk between signaling pathways in pathogen defense. Curr Opin Plant Biol, 2002, 5: 325–331
[22]Patil M A, Pierce M L, Phillips A L, Venters B J, Essenberg M. Identification of genes up-regulated in bacterial-blight-resistant upland cotton in response to inoculation with Xanthomonas campestris pv. malvacearum. Physiol Mol Plant Pathol, 2005, 67: 319–335
[23]Atsushi N, Chikara M, Mineo S, Hideyuki M, Atsushi K, Hong J S, Keisuke K, Jun A, Akira K. Functional analysis of soybean genes involved in flavonoid biosynthesis by virus-induced gene silencing. Plant Biotechnol J, 2007, 5: 778–790
[24]Boots A W, Haenen G R M M, Bast A. Health effects of quercetin: from antioxidant to nutraceutical. Eur J Pharmacol, 2008, 585: 325–337
[25]包改红, 毕阳, 李永才, 王毅, 王婷, 唐瑛, 马朝玲, 白小东. 硫色镰刀菌接种对抗病/易感品种马铃薯块茎苯丙烷代谢的影响比较. 食品科学, 2015, 36: 251–256
Bao G H, Bi Y, Li Y C, Wang Y, Wang T, Tang Y, Ma Z L, Bai X D. Comparison of phenylpropanoid pathway metabolism in slices of susceptible and resistant potato cultivars inoculated with Fusarium sulphureum. Food Sci, 2015, 36: 251–256 (in Chinese with English abstract)
[26]Guo S, Zuo Y, Zhang Y, Wu C, Su W, Jin W, Yu H, An Y, Li Q. Large-scale transcriptome comparison of sunflower genes responsive to Verticillium dahliae. BMC Genomics, 2017, 18: 42
[27]Tan B A, Daim L D J, Ithnin N, Ooi T E K, Md-Noh N, Mohamed M, Mohd-Yusof H, Appleton D R, Kulaveerasingam H. Expression of phenylpropanoid and Flavonoid Pathway genes in oil palm roots during infection by Ganoderma boninense. Plant Gene, 2016, 7: 11–20
[28]Sun W G, Xu Y. Study on the composition of the pigments in the nature colored cotton. J Xi’an Polytechnic Univ, 2009, 23:119–124
[29]Sun Q, Jiang H Z, Zhu X Y, Wang W N, He X H, Shi Y Z,Yuan Y L, Du X M, Cai Y F. Analysis of sea-island cotton and upland cotton in response to Verticillium dahliae infection by RNA sequencing. BMC Genomics, 2013, 14: 852
[30]Federico P, Marta N, María A B, Fuencisla M, Barceló A R. Changes in stem lignins (monomer composition and crosslinking) and peroxidase are related with the maintenance of leaf photosynthetic integrity during Verticillium wilt in Capsicum annuum. New Phyt, 2004, 163: 111–123
[31]Gayoso C, Pomar F, Novo-Uzal E, Merino F, Martínez D I O. The Ve-mediated resistance response of the tomato to Verticillium dahliae involves H2O2, peroxidase and lignins and drives PAL gene expression. J Med Hum, 2010, 10: 1–19
[32]Irina O V, Robert T R, Jane E G. David J R, Kent A M, Nicholi V. Characterization of flavonols in Vaccinium macrocarpon powder. J Agric Food Chem, 2003, 52: 188–195
[33]Li F, Jin Z, Qu W, Zhao D, Ma F. Cloning of a cDNA encoding the Saussurea medusa chalcone isomerase and its expression in transgenic tobacco. Plant Physiol Biochem, 2006, 44: 455–461
[34]Fawe A, Abou Z M, Menzies J G, Belanger R R. Silicon-mediated accumulation of flavonoid phytoalexins in cucumber. Phytopathology, 1998, 88: 396–401
[35]马春雷, 乔小燕, 陈亮. 茶树无色花色素还原酶基因克隆及表达分析. 茶叶科学, 2010, 30: 27–36
Ma C L, Qiao X Y, Chen L. Cloning and expression analysis of Leucoanthocyantin reducase gene of tea plant (Camellia sinensis). J Tea Sci, 2010, 30: 27–36 (in Chinese with English abstract)
[36]宋凤鸣, 郑重, 童贤明, 葛秀春. 儿茶素对棉枯萎病菌胞壁降解酶的抑制及在棉花抗病性中的作用. 真菌学报, 1996, 4: 297–303
Song F M, Zheng Z, Tong X M, Ge X C. Inhibition of tea catechins on cell wall degrading enzymes of cotton Fusarium Wilt and its role in disease resistance of cotton. Mycosystema, 1996, 4: 297–303 (in Chinese with English abstract)
[37]Shih, C H C I K, Yip, W K L, Clive. Differential expression of two flavonoid 3’-hydroxylase cDNAs involved in biosynthesis of anthocyanin pigments and 3’-deoxyanthocyanidin phytoalexins in sorghum. Plant Cell Physiol, 2006, 47: 1412–1419
[38]张松焕, 李春奇, 郭惠明, 裴熙祥, 程红梅. 过量表达紫茎泽兰类黄酮3’-羟化酶基因对转基因烟草POD、PAL活性的影响. 中国农业科技导报, 2009, 11: 98–101
Zhang S H, Li C Q,Guo H M, Pei X X, Cheng H M. Effects of eupatorium adenophorum flavonoid 3'-hydroxylase overexpression on pod and pal activity in transgenic tobacco. J Agric Sci Technol, 2009, 11: 98–101 (in Chinese with English abstract)
[39]Deshika K, Gopal J, Amit A D, Ankur R. B, Manu A, Surekha K A, Ramamurthy S, Pradeep K J. Identification and characterization of wilt and salt stress-responsive MicroRNAs in Chickpea through high-throughput sequencing. J B Inst Technol, 2014, 9: e108851

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