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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (12): 1791-1801.doi: 10.3724/SP.J.1006.2017.01791

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Correlation between Resistance to Fusarium Wilt and Expression of Flavonoid Metabolism Related Genes in Gossypium barbadense L.

HUANG Qi-Xiu,QU Yan-Ying,YAO Zheng-Pei,LI Meng-Yu,CHEN Quan-Jia*   

  1. College of Agronomy, Xinjiang Agriculture University / Key Laboratory of Agriculture Biological Technology, Xinjiang Agricultural University, Urumqi 830052
  • Received:2017-03-09 Revised:2017-07-23 Online:2017-12-12 Published:2017-08-10
  • Contact: 陈全家, E-mail: chqjia@126.com E-mail:13899525177@163.com
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31560409).

Abstract:

Fusarium wilt is one of the important factors that threaten the production of Gossypium barbadense. L. To expanding the molecular mechanism of resistance to Fusarium wilt will provide a solid foundation for cultivating resistant island cotton varieties and solve the problem of wilt disease. In this study DEG(Differentially Expressed Gene) was analyzed on the basis of sequencing of transgenic plants. The Disease resistance differentially expressed genes were analyzed at different inoculation time points of seven Gossypium barbadense. L varieties with different disease resistance levels, and the correlation between gene expression and disease index was analyzed. Flavonoid biosynthetic pathway genes were related to fusarium wilt resistance. qRT-PCR analysis showed that the expression of flavonoid metabolic pathway genes in resistant materials was significantly higher than that in susceptible materials. The expression levels of TT7, CHI and DFR, the key genes in flavonoid metabolic pathways, were significantly higher in resistant materials than in susceptible materials at multiple time points after inoculation, And the expression of CHI and DFR genes was negatively correlated with disease index. In summary, the flavonoid metabolic pathway-related genes have an effect on the resistance to fusarium wilt, among which CHI, TT7 and DFR genes are the key genes.

Key words: Gossypium barbadense, Fusarium wilt, Flavonoids, Expression analysis, RNA-seq

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