甘蓝型油菜芸薹素唑耐受因子(BnaBZR1/BnaBES1)全长CDS克隆与生物信息学分析

doi:10.3724/SP.J.1006.2018.01793

Abstract

Abstract:

Brassinazole-resistant (BZR) is a key transcription factor in brassinosteroid signaling pathway of plants, containing brassinazole-resistant 1 (BZR1) and brassinazole-resistant 2 (BES1). In this study, three novel BZR1 coding sequences (CDSs) were isolated from cDNA of Brassica napus L. cv. Xiangyou 15 leaves, which were mapped on the chromosomes A07, A06, and A06, and designated as BnaBZR1_A07, BnaBES1_A06F, and BnaBES1_A06R, respectively. These three BnaBZR CDSs were 996, 993, and 996 bp in length and encoded predicted proteins with 331, 330, and 331 amino acid residues, respectively. BnaBZR proteins were predicted to be located on the cell nucleus and have a typical plant BZR/BES conserved domain. Multiple sequence alignments and phylogenetic analysis showed that the deduced amino acid sequences of BnaBZR were highly homologous to previously reported BZR/BES of Brassica oleracea, Arabidopsis thalian, and Eruca sativa. And the similarity of BZR1 or BES1 among different related species was higher than the similarity between BZR1 and BES1 in the same species or related species, indicating that BZR1 and BES1 differentiation is an early evolutionary event. The expression patterns of BnaBZR1_A07, BnaBES1_A06F, and BnaBES1_A06R in Xiangyou 15 were similar, whit high expression level at the seedling stage and flowering stage, while slightly lower level at the stage of bolting and podgrain ripening. The expression level of BnaBZR in the aerial parts such as leaves, stems, flowers and pods was higher than that in underground parts.

Key words: Brassica napus L., BZR, gene clone, gene expression, bionformatic analysis

Tao FENG,Chun-Yun GUAN. Cloning and Characterization of Brassinazole-resistant (BnaBZR1 and BnaBES1) CDS from Brassica napus L.[J].Acta Agronomica Sinica, 2018, 44(12): 1793-1801.

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蛋白编号 Serial number	氨基酸残基数 Base of amino acid residues	摩尔质量 Molar mass (Da)	等电点 Isoelectric point
BnaBZR1_A07	331	35 895.131	9.145
BnaBES1_A06F	330	36 071.176	9.745
BnaBES1_A06R	331	36 177.322	9.745

蛋白编号 Serial number	位点类型 Domains type		数量 Number	位置 Location	序列 Sequence	序列模式 Sequence motif
BnaBZR1_A07	PS00001		2	142-145, 143-146	NNSS, NSST	N-{P}-[ST]-{P}
	PS00004		1	21-24	RKPS	[RK](2)-x-[ST]
	PS00005		5	24-26, 84-86, 162-164, 179-181, 221-223	SWR, TYR, SLR, TSK, THR	[ST]-x-[RK]
	PS00006		4	24-27, 236-239, 230-233, 240-243	SWRE, SRGE, TIPE, STVD	[ST]-x(2)-[DE]
	PS00008		4	5-10, 154-159, 155-160, 325-330	GATSTS, GGIPSS, GIPSSSL, GNGKAR	G-{EDRKHPFYW}-x(2)-[STAGCN]-{P}
BnaBES1_A06F	PS00001		1	138-141	NIST	N-{P}-[ST]-{P}
	PS00004		1	20-23	RKPS	[RK](2)-x-[ST]
蛋白编号 Serial number	位点类型 Domains type		数量 Number	位置 Location	序列 Sequence	序列模式 Sequence motif
BnaBES1_A06R	PS00005	5		18-20, 23-25, 83-85, 99-101, 174-176	TRR, SWR, TYR, SSR, TNK	[ST]-x-[RK]
	PS00006	6		23-26, 112-115, 134 -137, 227 -230, 237-240, 305-308	SWRE, SPFE, SRGD, TIPE, STVD, TPWE	[ST]-x(2)-[DE]
	PS00008	5		5-10, 149-154, 150-155, 259-264, 325-330	GATSTS, GGIPSS, GIPSSL, GVSSAV, GNAKGR	G-{EDRKHPFYW}-x(2)-[STAGCN]-{P}
	PS00016	1		135-137	RGD	R-G-D
	PS00001	1		139-142	NIST	N-{P}-[ST]-{P}
	PS00004	1		21-24	RKPS	[RK](2)-x-[ST]
	PS00005	5		18-20, 24-26, 84-86, 100-102, 175-177	TRR, SWR, TYR, SSR, TSK	[ST]-x-[RK]
	PS00006	6		24-27, 113-116, 135-138, 228-231, 238-241, 306-309	SWRE, SPFE, SRGD, TIPE, STVD, TPWE	[ST]-x(2)-[DE]
	PS00008	5		5-10, 150-155, 151-156, 260-265, 326-331	GATSTS, GGIPSS, GIPSSL, GVSSAV, GNAKGR	G-{EDRKHPFYW}-x(2)-[STAGCN]-{P}
	PS00016	1		136-138	RGD	R-G-D

Cloning and Characterization of Brassinazole-resistant (BnaBZR1 and BnaBES1) CDS from Brassica napus L.

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