Welcome to Acta Agronomica Sinica，

Acta Agronomica Sinica ›› 2020, Vol. 46 ›› Issue (4): 513-519.doi: 10.3724/SP.J.1006.2020.91051

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic stability of wheat-rye 6RS/6AL translocation chromosome and its transmission through gametes

LI Qing-Cheng,HUANG Lei,LI Ya-Zhou,FAN Chao-Lan,XIE Die,ZHAO Lai-Bin,ZHANG Shu-Jie,CHEN Xue-Jiao,NING Shun-Zong,YUAN Zhong-Wei,ZHAN Lian-Quan,LIU Deng-Cai,HAO Ming()   

  1. Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
  • Received:2019-08-07 Accepted:2019-12-26 Online:2020-04-12 Published:2020-01-16
  • Contact: Ming HAO E-mail:haomingluo@foxmail.com
  • Supported by:
    This study was supported by the Key Projects of Sichuan Provincial Department of Education(16ZA0028);the Applied Basic Research Programs of Science & Technology Department of Sichuan Province(2019YJ0415)

RichHTML367

31

PDF

804

Abstract:

The wheat-rye 6RS/6AL translocation line HM812-41 harbors the powdery mildew resistance gene Pm56. In order to evaluate the potential utilization in breeding, the translocation line was crossed to common wheat varieties Shumai 580, Shumai 830, and Shumai 969. The F1 hybrids were then reciprocally crossed with Chinese spring to estimate the genetic stability of 6RS/6AL and its transmission rate through male and female gametes in different genetic backgrounds. Double-top crossing (DTC) strategy was used to improve the agronomic traits of the translocation line. Genome in situ hybridization analysis indicated that the 6RS/6AL translocation was very stable during transmission. The 6RS/6AL translocation was transmitted to offsprings with a high frequency, which was 45.05%-53.33% for female gametes and 43.94%-53.04% for male gametes. Based on the agronomical performances of DTC F2 populations, we found that the 6RS/6AL translocation was not linked to obvious defects for major agronomic traits, such as plant height, spike length, spikelet number and seed-setting ratio in selfing. The agronomic traits of the translocation line can be obviously improved through DTC strategy.

Key words: wheat, rye (Secale cereal), powdery mildew, 6RS/6AL translocation, gamete transmission, genetic stability

Fig. 1

Centromeres of 6RS/6AL translocation lines A: 42 chromosomes of the translocation line HM812-41; B: centromeres of the translocation line HM812-41 detected by the probe 6C6; C: centromeres of the translocation line HM812-41 detected by the probe pAWRC.1; D: merging of B and C. Arrows show the centromeres of the translocated chromosome 6RS/6AL."

Table 1

Transmission rate of 6RS/6AL through gametes in wheat background"

组合
Combination		 传递方式
Type of
transmission		 植株总数
No. of total plants		 含标记植株数
No. of plants with 6RS		 传递率
Ratio of transmission (%)		 χ2
(蜀麦580×HM812-41)×中国春
(Shumai 580×HM812-41)×CS		 雌配子Female 91 41 45.05 0.35
中国春×(蜀麦580×HM812-41)
CS×(Shumai 580×HM812-41)		 雄配子Male 93 43 46.24 0.47
(蜀麦830×HM812-41)×中国春
(Shumai 830×HM812-41)×CS		 雌配子Female 120 64 53.33 0.47
中国春×(蜀麦830×HM812-41)
CS×(Shumai 830×HM812-41)		 雄配子Male 115 61 53.04 0.51
(蜀麦969×HM812-41)×中国春
(Shumai 969×HM812-41)×CS		 雌配子Female 75 36 48.00 0.73
中国春×(蜀麦969×HM812-41)
CS×(Shumai 969×HM812-41)		 雄配子Male 66 29 43.94 0.32

Fig. 2

Crossing diagram for improving 6RS/6AL translocation line and the progenies' performance A: Crossing diagram for improving 6RS/6AL translocation line; B: the progenies’ performance. The plants from left to right are the translocation line HM812-41, Shumai 580, Shumai 830, Shumai 969, Shumai 969/HM812-41 F1, Shumai 969/HM812-41//Shumai 830 F1, Shumai 969/HM812-41//Shumai 830/3/WJL3931 F1, and Shumai 969/HM812-41//Shumai 830/3/WJL3931 F2, respectively."

Fig. 3

Performances of major traits for two DTC F2 populations A: plant height; B: spike length; C: spikelet number; D: seed set ratio. Pop.1+ and Pop.1-, represent sub-population with and without 6RS/6AL translocation from Shumai 969/HM812-41//Shumai 830/3/WJL3931 F2 population, respectively. Pop.2+ and Pop.2-, represent subpopulation with and without 6RS/6AL translocation from Shumai 969/HM812-41//WJL5606/3/Shumai 126 F2 population. **: significantly difference between sub-populations with and without 6RS/6AL translocation (two-way t-test, P < 0.01)."

[1] Ehdaie B, Whitkus R W, Waines J G . Root biomass, water-use efficiency, and performance of wheat-rye translocations of chromosomes 1 and 2 in spring bread wheat ‘Pavon’. Crop Sci, 2003,43:710-717.
[2] Sharma S, Xu S, Ehdaie B, Hoops A, Close T J, Lukaszewski A J, Waines J G . Dissection of QTL effects for root traits using a chromosome arm-specific mapping population in bread wheat. Theor Appl Genet, 2011,122:759-769.
[3] 张爱民, 童依平, 王道文 . 小麦遗传育种学家李振声. 遗传, 2008,30:1239-1240.
Zhang A M, Tong Y P, Wang D W . Wheat geneticist Li Zhensheng. Hereditas, 2008,30:1239-1240 (in Chinese).
[4] Li Z S, Li B, Tong Y . The contribution of distant hybridization with decaploid Agropyron elongatum to wheat improvement in China. J Genet Genom, 2008,35:451-456.
[5] 陈佩度, 张守忠, 王秀娥, 王苏玲, 周波, 冯祎高, 刘大钧 . 抗白粉病高产小麦新品种南农9918. 南京农业大学学报, 2002,25:105-106.
Chen P D, Zhang S Z, Wang X E, Wang S L, Zhou B, Feng Y G, Liu D J . New wheat variety Nannong 9918 with high yield and powdery mildew resistance. J Nanjing Agric Univ, 2002,25:105-106 (in Chinese with English abstract).
[6] Cao A, Xing L, Wang X, Yang X, Wang W, Sun Y, Qian C, Ni J, Chen Y, Liu D, Wang X, Chen P . Serine threonine kinase gene Stpk-V, a key member of powdery mildew resistance gene Pm21, confers powdery mildew resistance in wheat. Proc Natl Acad Sci USA, 2011,108:7727-7732.
[7] 李立会, 杨欣明, 李秀全, 董玉琛, 陈学明 . 通过属间杂交向小麦转移冰草优异基因的研究. 中国农业科学, 1998,31(6):1-6.
Li L H, Yang X M, Li X, Dong Y C, Chen X M . Introduction of desirable genes from Agropyron cristatum into common wheat by intergeneric hybridization. Sci Agric Sin, 1998,31(6):1-6 (in Chinese with English abstract).
[8] 杜丽媛, 刘伟华, 杨欣明, 李秀全, 李立会 . 小麦-冰草新种质普冰2011姊妹系的育种效应分析. 植物遗传资源学报, 2016,17:395-403.
Du L Y, Liu W H, Yang X M, Li X Q, Li L H . Breeding achievement analysis of novel wheat- Agropyron cristatum germplasm sister lines pubbing 2011. J Plant Genet Resour, 2016,17:395-403 (in Chinese with English abstract).
[9] Sears E R. Chromosome engineering in wheat. In: Stadler Symp., Vol. 4, University of Missouri, Columbia, USA. 1972, pp 23-38.
[10] 李桂萍, 陈佩度, 张瑞奇, 王春梅, 曹爱忠, 张守忠 . 小麦-簇毛麦6VS/6AL易位染色体在不同小麦背景中的遗传稳定性及其在配子中的传递. 麦类作物学报, 2007,27:183-187.
Li G P, Chen P D, Zhang R Q, Wang C M, Cao A Z, Zhang S Z . Transmission of the 6VS/6AL chromosome through gametes and its genetic stability in different genetic background. J Triticeae Crops, 2007,27:183-187 (in Chinese with English abstract).
[11] 王海燕, 肖进, 袁春霞, 徐涛, 于春燕, 孙昊杰, 陈佩度, 王秀娥 . 携带抗白粉病基因Pm21的小麦-簇毛麦小片段易位染色体在不同小麦背景中的传递率及遗传稳定性. 作物学报, 2016,42:361-367.
Wang H Y, Xiao J, Yuan C X, Xu T, Yu C Y, Sun H J, Chen P D, Wang X E . Transmission and genetic stability of no-homoeologous small fragment wheat- Haynaldia villosa translocation chromosomes with Pm21 in various cultivar backgrounds of common wheat. Acta Agron Sin, 2016,42:361-367 (in Chinese with English abstract).
[12] 李桂萍, 陈佩度, 张守忠, 赵和 . 小麦-簇毛麦6VS/6AL易位染色体对小麦农艺性状的影响. 植物遗传资源学报, 2011,12 : 744-749.
Li G P, Chen P D, Zhang S Z, Zhao H . Effects of the 6VS/6AL translocation chromosome on agronomic characteristics of wheat. J Plant Genet Resour, 2011,12:744-749 (in Chinese with English abstract).
[13] Zhao R, Liu B, Jiang Z, Chen T, Wang L, Ji Y, Hu Z, He H, Bie T . Comparative analysis of genetic effects of wheat-Dasypyrum villosum translocations T6V#2S·6AL and T6V#4S·6DL. Plant Breed, 2019, .
[14] Martis M M, Zhou R, Haseneyer G, Schmutzer T, Vrána J, Kubaláková M, König S, Kugler K G, Scholz U, Hackauf B, Korzun V, Schön C C, Dolezel J, Bauer E, Mayer K F X, Stein N . Reticulate evolution of the rye genome. Plant Cell, 2013,25:3685-3698.
[15] Heun M . Introgression of powdery mildew resistance from rye into wheat. Phytopathology, 1990,80:242-245.
[16] Friebe B, Heun M, Tuleen N, Zeller F J, Gill B S . Cytogenetically monitored transfer of powdery mildew resistance from rye into wheat. Crop Sci, 1994,34:621-625.
[17] Hsam S L K, Zeller F J . Evidence of allelism between genes Pm8 and Pm17 and chromosomal location of powdery mildew and leaf rust resistance genes in the common wheat cultivar Amigo. Plant Breed, 1997,116:119-122.
[18] Ren T H, Yang Z J, Yan B J, Zhang H Q, Fu S L, Ren Z L . Development and characterization of a new 1BL.1RS translocation line with resistance to stripe rust and powdery mildew of wheat. Euphytica, 2009,169:207-213.
[19] An D, Zheng Q, Zhou Y, Ma P, Lv Z, Li L, Li B, Luo Q, Xu H, Xu Y . Molecular cytogenetic characterization of a new wheat-rye 4R chromosome translocation line resistant to powdery mildew. Chrom Res, 2013,21:419-432.
[20] Fu S L, Ren Z L, Chen X M, Yan B J, Tan F Q, Fu T H, Tang Z X . New wheat-rye 5DS-4RS·4RL and 4RS-5DS·5DL translocation lines with powdery mildew resistance. J Plant Res, 2014,127:743-753.
[21] Hao M, Liu M, Luo J, Fan C, Yi Y, Zhang L, Yuan Z, Ning S, Zheng Y, Liu D . Introgression of powdery mildew resistance gene Pm56 on rye chromosome arm 6RS into wheat. Front Plant Sci, 2018,9:1040.
[22] Hao M, Zhang L, Zhao L, Dai S, Li A, Yang W, Xie D, Li Q, Ning S, Yan Z, Wu B, Lan X, Yuan Z, Huang L, Wang J, Zheng K, Chen W, Yu M, Chen X, Chen M, Wei Y, Zhang H, Kishii M, Hawkesford M J, Mao L, Zheng Y, Liu D . A breeding strategy targeting the secondary gene pool of bread wheat: introgression from a synthetic hexaploid wheat. Theor Appl Genet, 2019,132:2285-2294.
[23] 刘英华, 赵桃, 龙海, 邓光兵, 潘志芬, 余懋群 . 簇毛麦(Dasypyrum villosum)矮秆突变体的鉴定. 麦类作物学报, 28:946-949.
Liu Y H, Zhao T, Long H, Deng G B, Pan Z F, Yu M Q . Characterization of a dwarf mutant in Dasypyrum villosum. J Triticeae Crops, 2008,28:946-949 (in Chinese with English abstract).
[24] Yan Z, Wan Y, Liu K . Identification of a novel HMW glutenin subunit and comparison of its amino acid sequence with those of homologous subunits. Chin Sci Bull, 2002,47:220-225.
[25] Qiu L, Tang Z X, Li M, Fu S L . Development of new PCR-based markers specific for chromosome arms of rye ( Secale cereale L.). Genome, 2016,59:159-165.
[26] 邹晓欢, 郝明, 罗江陶, 张连全, 袁中伟, 刘登才 . 中国特有小麦的易位染色体鉴定. 麦类作物学报, 33:1065-1070.
Zou X H, Hao M, Luo J T, Zhang L Q, Yuan Z W, Liu D C . Identification of translocated chromosomes in Chinese endemic wheat accessions. J Triticeae Crops, 2013,33:1065-1070 (in Chinese with English abstract).
[27] 唐宗祥 . 重复序列引起小麦染色体结构、基因组及性状的改变. 四川农业大学博士学位论文, 2006, 四川雅安.
Tang Z X . Variation of Chromosomal Structure, Genome and Phenotype of Wheat Caused by Repetitive DNA Sequence. PhD Dissertation of Sichuan Agricultural University, Ya’an, Sichuan, China, 2006 (in Chinese with English abstract).
[28] Hao M, Luo J, Yang M, Zhang L, Yan Z, Yuan Z, Zheng Y, Zhang H, Liu D . Comparison of homoeologous chromosome pairing between hybrids of wheat genotypes Chinese Spring ph1b and KL with rye. Genome, 2011,54:959-964.
[29] 张文俊, 景健康, 胡含 . 黑麦6R染色体在小麦背景中的传递. 遗传学报, 1995,22:211-216.
Zhang W J, Jing J K, Hu H . The transmission of rye chromosome 6R in wheat background. Acta Genet Sin, 1995,22:211-216 (in Chinese with English abstract).
[1] Zhai Sheng-Nan, Cao Xin-You, Li Hao-Sheng, Li Ji-Hu, Li Fa-Ji, Liu Jin-Dong, Xia Xian-Chun, Lyu Ying-Ying, Ma Rui-Feng, Wang Ying, Geng Hong-Wei, Liu Jian-Jun. Analysis of the genetic effects of allelic variation at the Pod-A1, Pod-D1, and Pod-2D loci on peroxidase activity in wheat grains [J]. Acta Agronomica Sinica, 2026, 52(6): 1593-1603.
[2] Xi Qian-Hui, Xu Zi-Yuan, Liu Meng-Meng, Wang Hong-Yi, Lang Kai-Lin, Jing Zhen-Hai, Chen Feng, Zhao Lei. Genome-wide association study and candidate gene prediction of grain copper content in wheat [J]. Acta Agronomica Sinica, 2026, 52(6): 1604-1617.
[3] Mao Jia-Qi, Huang Peng-Yu, Zhao Jia-Jia, Zheng Xing-Wei, Wu Bang-Bang, Hao Yu-Qiong, Qu Fei, Liu Cheng, Ma Peng-Tao, Zheng Jun. Evaluation of powdery mildew resistance in wheat cultivars and molecular detection of resistance genes in Shanxi province, China [J]. Acta Agronomica Sinica, 2026, 52(6): 1669-1681.
[4] Hu Chuan, Zhao Kai-Nan, Huang Xiu-Li, Wu Jin-Zhi, Ren Kai-Ming, Wang He-Zheng, Fu Guo-Zhan, Huang Ming, Li You-Jun. Effects of tillage methods and nitrogen rates on yield and quality of dryland wheat under one-off irrigation [J]. Acta Agronomica Sinica, 2026, 52(6): 1830-1846.
[5] Chen Xue-Yan, He Hua-Chuan, Li Zheng-Jia, Dong Xin-Pan, Li Ou-Qi, Liu Xiao-Yun, Li Dan-Ping, Chen Zhi-Wei, Liu Guo-Xia, Lyu Sheng-Yuan, Wu Yin-Ying, Zhao Zhen-Dong, Cao Xin-You, Wan He-Ping. Dynamic changes in root organic acid secretion and its transcriptional regulatory mechanisms in ‘Jimai 60’ seedlings under combined salinity-alkalinity stress in hydroponics [J]. Acta Agronomica Sinica, 2026, 52(6): 1859-1875.
[6] Gao Pei-Yang, Li Jin-Xuan, Dong Yu-Kui, Shi Yu, Zhang Zhen, Zhang Yong-Li. Response of wheat tillering and spike formation to nitrogen rate under supplementary irrigation based on soil moisture content [J]. Acta Agronomica Sinica, 2026, 52(6): 1847-1858.
[7] Zhang Xian-Feng, Guo Li-Jian, Li Kang-Chun, Kong Bin-Xue, Liu Yu-Fang, Che Zhuo, Yang De-Long. Identification of the ABHD6 gene family and development of functional markers for grain weight in wheat [J]. Acta Agronomica Sinica, 2026, 52(6): 1711-1727.
[8] Wang Zhuang-Zhuang, Wu Zi-Jun, Zhang Yong-Xin, Zhang Xin-Yuan, Yuan Li-Xue, Chen Ru-Xue, Liu Shi-Ju, Duan Jian-Zhao, Feng Wei, Wang Tong-Chao, Wang Yong-Hua. Optimized water-nitrogen synergy enhances winter wheat yield and nitrogen use efficiency in clay-loam fluvo-aquic soils of southeastern Henan, China [J]. Acta Agronomica Sinica, 2026, 52(5): 1501-1521.
[9] He Wan-Long, Geng Hong-Wei, Zhang Fei-Fei, Mikereayi·Ababaikere , Luo Zi-Yang, Li Peng-Cheng, Zhou Zhao-Yu, Cheng Yu-Kun. Development of a deep learning-based image recognition system for major wheat diseases [J]. Acta Agronomica Sinica, 2026, 52(5): 1401-1417.
[10] Zhang Zhen, Feng Lian-Jie, Shi Yu, Yu Zhen-Wen, Zhang Yong-Li. Yield formation of wheat with different ear types under water-saving supplementary irrigation conditions [J]. Acta Agronomica Sinica, 2026, 52(5): 1522-1535.
[11] Hou Si-Yu, Wang Guo-Cui, Wei Jin-Gui, Xie Wei-Xin, Yin Wen, Fan Zhi-Long, Chai Qiang, Hu Fa-Long. Effects of green manure combined with chemical nitrogen fertilizer on dry matter accumulation and yield formation of wheat in arid irrigation areas of northwestern China [J]. Acta Agronomica Sinica, 2026, 52(4): 1208-1219.
[12] Shang Yun-Qiu, Zhao Zhu, Chen Huan, Ding Yong-Gang, Qiao Yu-Qiang, Li Wei, Zhang Xiang-Qian, Cao Cheng-Fu, Du Shi-Zhou. Effects of long-term tillage practices on grain-filling and yield formation in rain-fed wheat [J]. Acta Agronomica Sinica, 2026, 52(4): 1236-1250.
[13] Qiao Yu-Xin, Li Cheng-Yue, Kang Xiao-Yu, Zhang Xin-Qi, Jia Shao-Hui, Liu Qian, Cao Ya-Li, Shi Xin-Rui, Hao Xing-Yu, Li Ping. Study on the effects of long-term no-tillage straw mulching on wheat yield improvement in dryland areas based on the APSIM model [J]. Acta Agronomica Sinica, 2026, 52(4): 1181-1192.
[14] Li Can, Zhang Xi-Wei, Zhu Bo-Tao, Zhang Pei-Pei. Functional characterization of wheat GSK kinase TaSK41 and screening for interacting proteins [J]. Acta Agronomica Sinica, 2026, 52(3): 677-687.
[15] Hou Jie, Fu Duo-Duo, Wu Hai-Feng, Hao Yu-Qiong, Zheng Xing-Wei, Wu Bang-Bang, Zhou Kai, Li Xiao-Hua, Zheng Jun, Zhao Jia-Jia. Chromosome diversity and its effects in wheat landraces from Shanxi province, China [J]. Acta Agronomica Sinica, 2026, 52(3): 746-763.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd